中国组织工程研究 ›› 2022, Vol. 26 ›› Issue (28): 4519-4524.doi: 10.12307/2022.308

• 复合支架材料 composite scaffold materials • 上一篇    下一篇

Ⅱ型胶原-丝素蛋白-透明质酸复合支架的制备及力学性能检测

刘  钢1,2,高丽兰1,2,3,史菲菲4,王世鑫3,罗来龙3,李瑞欣5,张春秋1,2   

  1. 1天津市先进机电系统设计与智能控制重点实验室,天津理工大学机械工程学院,天津市  300384;2机电工程国家级实验教学示范中心(天津理工大学),天津市  300384;3天津市透明质酸应用研究企业重点实验室,天津市  300457;4北斗(天津)夹具装备有限公司,天津市  300300;5天津市口腔医院,天津市  300041
  • 收稿日期:2021-03-20 接受日期:2021-04-23 出版日期:2022-10-08 发布日期:2022-03-21
  • 通讯作者: 高丽兰,教授,博士,天津市先进机电系统设计与智能控制重点实验室,天津理工大学机械工程学院,天津市 300384;机电工程国家级实验教学示范中心(天津理工大学),天津市 300384;天津市透明质酸应用研究企业重点实验室,天津市 300457
  • 作者简介:刘钢,男,1995年生,内蒙古自治区呼伦贝尔市人,汉族,天津理工大学在读硕士,主要从事生物材料力学方向研究。
  • 基金资助:
    天津市透明质酸应用研究企业重点实验室开放基金(KTRDHA-Y201905),项目负责人:高丽兰,国家自然科学基金资助项目(11972198),项目负责人:李瑞欣,国家自然科学基金资助项目(12072235),项目负责人:张春秋

Preparation and mechanical properties of collagen type II-silk fibroin-hyaluronic acid composite scaffold

Liu Gang1, 2, Gao Lilan1, 2, 3, Shi Feifei4, Wang Shixin3, Luo Lailong3, Li Ruixin5, Zhang Chunqiu1, 2   

  1. 1Tianjin Key Laboratory of Advanced Electromechanical System Design and Intelligent Control, Tianjin 300384, China; 2National Experimental Teaching Demonstration Center of Mechanical and Electrical Engineering (Tianjin University of Technology), Tianjin 300384, China; 3Tianjin Enterprise Key Laboratory on Hyaluronic Acid Application Research, Tianjin 300457, China; 4Beidou (Tianjin) Fixture Equipment Co., Ltd., Tianjin 300300, China; 5Tianjin Stomatological Hospital, Tianjin 300041, China
  • Received:2021-03-20 Accepted:2021-04-23 Online:2022-10-08 Published:2022-03-21
  • Contact: Gao Lilan, MD, Professor, Tianjin Key Laboratory of Advanced Electromechanical System Design and Intelligent Control, School of Mechanical Engineering, Tianjin University of Technology, Tianjin 300384, China; National Experimental Teaching Demonstration Center of Mechanical and Electrical Engineering (Tianjin University of Technology), Tianjin 300384, China; Tianjin Enterprise Key Laboratory on Hyaluronic Acid Application Research, Tianjin 300457, China
  • About author:Liu Gang, Master candidate, Tianjin Key Laboratory of Advanced Electromechanical System Design and Intelligent Control, School of Mechanical Engineering, Tianjin University of Technology, Tianjin 300384, China; National Experimental Teaching Demonstration Center of Mechanical and Electrical Engineering (Tianjin University of Technology), Tianjin 300384, China
  • Supported by:
    Key Laboratory Open Fund of Hyaluronic Acid Application Research Enterprise of Tianjin, No. KTRDHA-Y201905 (to GLL); the National Natural Science Foundation of China, No. 11972198 (to LRX), No. 12072235 (to ZCQ)

摘要:

文题释义:
透明质酸:作为天然细胞外基质中糖胺多糖的重要组成物,具有高度水化的性能,在对调节组织内部的水分平衡、关节的润滑及稳定软骨基质等方面起到了重要作用,在关节软骨在负重时,透明质酸网状结构的存在可预防大量的液体自软骨中被挤出,保证软骨内的水分,从而保证软骨的弹性及缓冲应力的作用。
3D打印技术:是一种快速成型技术,利用计算机三维设计软件重建图像模型。根据各种参数,将Ⅱ型胶原、丝素蛋白和透明质酸3种材料以一定的厚度逐层打印并堆积形成三维支架,3D打印技术可以精确地设计并控制支架结构,打印出来的复合支架形状规则、孔径均一、连通性好。

背景:Ⅱ型胶原、丝素蛋白和透明质酸3种天然生物可降解材料能够为细胞提供理想的微环境,已成为理想的软骨修复支架材料。
目的:将Ⅱ型胶原、丝素蛋白和透明质酸3种材料制备成软骨组织工程支架,评价其理化性质及生物力学性能。
方法:采用低温3D打印技术制备Ⅱ型胶原-丝素蛋白-透明质酸复合支架,检测其微观结构、孔隙率与吸水膨胀率。采用不同的应变率对该复合支架进行压缩实验,考察支架的率相关性能;在该复合支架表面施加恒定的应力水平或恒定的应变,保持3 600 s,观察其蠕变应变变化及应力松弛行为。
结果与结论:①Ⅱ型胶原-丝素蛋白-透明质酸复合支架呈三维多孔结构,孔径大小一致,相通性好,孔隙率为(85.1±1.6)%,吸水膨胀率为(1 071.7±131.6)%;②在不同的应变率作用下,软骨支架的压缩应力-应变曲线不重合,说明软骨支架的压缩力学性能具有率相关性;随着应变率的增加,复合支架的杨氏模量增加;③当应力水平恒定时,复合支架的蠕变应变呈现先快速增加后缓慢增加的趋势,当应力水平增加时蠕变应变也增加;④当压缩应变恒定时,支架的应力随着松弛时间先快速降低后缓慢降低,随着压缩应变的增大,不同时刻的应力都增大;⑤力学性能实验表明,制备的Ⅱ型胶原-丝素蛋白-透明质酸软骨支架力学性能特征与宿主软骨组织相似,都是非线性黏弹性材料。

https://orcid.org/0000-0001-5102-2224 (刘钢);https://orcid.org/0000-0002-7288-6686 (高丽兰) 

中国组织工程研究杂志出版内容重点:生物材料;骨生物材料口腔生物材料纳米材料缓释材料材料相容性组织工程

关键词: 软骨支架, Ⅱ型胶原, 丝素蛋白, 透明质酸, 率相关, 蠕变, 应力松弛

Abstract: BACKGROUND: Collagen type II, silk fibroin and hyaluronic acid, which can provide an ideal microenvironment for cells, have become ideal scaffold materials for cartilage repair.
OBJECTIVE: To evaluate physicochemical properties and biomechanical properties of cartilage tissue engineering scaffolds prepared from collagen type II, silk fibroin and hyaluronic acid.
METHODS: Collagen type II-silk fibroin-hyaluronic acid composite scaffolds were prepared by low-temperature 3D printing technology. The microstructure, porosity and water absorption of scaffold were detected. Different strain rates were used to compress the scaffold, and the rate dependent properties of the scaffold were investigated. Constant stress level or constant strain was applied on the surface of scaffold for 3 600 s, and the creep behavior and stress relaxation behavior were investigated.
RESULTS AND CONCLUSION: (1) The prepared scaffolds had three-dimensional porous structure, uniform pore size and good connectivity. The porosity was (85.1±1.6)%. The water absorption expansion rate was (1 071.7±131.6)%. (2) Under different strain rates, the compressive stress-strain curves of cartilage scaffolds did not coincide, which indicated that the compressive mechanical properties of cartilage scaffolds were rate dependent. With the increase of strain rate, the Young’s modulus increased. (3) When the stress level was constant, the creep strain first increased rapidly and then increased slowly. When the stress level increased, the creep strain also increased. (4) When the compression strain was constant, the stress of scaffold decreased rapidly at first and then slowly with the relaxation time. With the increase of the compression strain, the stress at different times increased. (5) The mechanical properties of the collagen type II-silk fibroin-hyaluronic acid composite scaffolds are similar to those of host cartilage, which means that the prepared scaffold is a kind of nonlinear viscoelastic material.

Key words: cartilage scaffold, type II collagen, silk fibroin, sodium hyaluronate, rate dependent, creep, stress relaxation

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