中国组织工程研究

中国组织工程研究 ›› 2022, Vol. 26 ›› Issue (1): 27-32.doi: 10.12307/2022.005

• 骨髓干细胞 bone marrow stem cells • 上一篇    下一篇

脱细胞神经联合骨髓间充质干细胞及富血小板凝胶治疗股神经损伤

张学磊1，罗  干1，于圣会1，顾祖超1，彭  旭2，何学令2，刘  艳2，张晓梅2   

  1. 1成都市中西医结合医院/成都市第一人民医院，四川省成都市  610041；2四川大学实验动物中心，四川省成都市  610041
  • 收稿日期:2020-07-22 修回日期:2020-07-24 接受日期:2020-08-27 出版日期:2022-01-08 发布日期:2021-10-23
  • 通讯作者: 张晓梅，硕士，实验师，四川大学实验动物中心，四川省成都市 610041
  • 作者简介:张学磊，男，1986年生，山东省青岛市人，汉族，2016年四川大学华西临床医学院毕业，博士，主治医师，主要从事神经脊柱临床及基础研究工作。
  • 基金资助:
    成都中医药大学“杏林学者”学科人才科研提升计划项目(YYZX20180057)，项目负责人：张学磊

Acellular nerve scaffold combined with bone marrow mesenchymal stem cells and platelet-rich gel for femoral nerve injury

Zhang Xuelei1, Luo Gan1, Yu Shenghui1, Gu Zuchao1, Peng Xu2, He Xueling2, Liu Yan2, Zhang Xiaomei2   

  1. 1Chengdu Integrated TCM & Western Medicine Hospital /Chengdu First People’s Hospital, Chengdu 610041, Sichuan Province, China; 2Experimental Animal Center of Sichuan University, Chengdu 610041, Sichuan Province, China
  • Received:2020-07-22 Revised:2020-07-24 Accepted:2020-08-27 Online:2022-01-08 Published:2021-10-23
  • Contact: Zhang Xiaomei, Master, Experimentalist, Experimental Animal Center of Sichuan University, Chengdu 610041, Sichuan Province, China
  • About author:Zhang Xuelei, MD, Attending physician, Chengdu Integrated TCM & Western Medicine Hospital /Chengdu First People’s Hospital, Chengdu 610041, Sichuan Province, China
  • Supported by:
    the “Xinglin Scholar” Subject Talent Research Promotion Project of Chengdu University of TCM, No. YYZX20180057 (to ZXL) 

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摘要：

文题释义：

脱细胞神经支架：用化学和生物试剂对神经进行脱细胞萃取处理，清除神经的细胞和髓鞘成分，能够去除神经的免疫原性，同时保留神经的三维空间结构，获得的脱细胞神经纤维就是脱细胞神经支架。脱细胞神经支架具有良好的完整性、组织相容性及生物力学稳定性，是神经修复的理想支架材料。
骨髓间充质干细胞：是一种来源于骨髓，具有自我更新和多向分化能力的成体干细胞，在体外特定的条件下可以诱导分化为骨、软骨和脂肪细胞，能够通过转分化和旁分泌等多种方式参与机体的免疫调节和组织修复，对多种疾病显示出较好的治疗作用。

背景：用异体脱细胞神经支架联合骨髓间充质干细胞和富血小板凝胶治疗兔股神经损伤，目前报道较少，机制并不明确。
目的：评估脱细胞神经支架联合骨髓间充质干细胞和富血小板凝胶移植治疗兔股神经损伤的效果。
方法：将25只新西兰兔随机分为5组，分别为单纯断端吻合组、脱细胞神经桥接组、脱细胞神经联合富血小板凝胶组、脱细胞神经联合骨髓间充质干细胞组、脱细胞神经联合富血小板凝胶和骨髓间充质干细胞组，术后12周称量腓肠肌湿质量，然后进行神经组织病理学染色。

结果与结论：脱细胞神经联合富血小板凝胶和骨髓间充质干细胞组腓肠肌湿质量明显高于其他组(P < 0.05)，有较多的再生神经纤维生成，无炎性细胞浸润，神经丝蛋白NF-200阳性表达也较多，上述结果提示，脱细胞神经支架联合骨髓间充质干细胞和富血小板凝胶对于股神经损伤具有较好的修复效果。

https://orcid.org/0000-0001-6396-2125(张晓梅) 

中国组织工程研究杂志出版内容重点：干细胞；骨髓干细胞；造血干细胞；脂肪干细胞；肿瘤干细胞；胚胎干细胞；脐带脐血干细胞；干细胞诱导；干细胞分化；组织工程

关键词: 干细胞, 脱细胞神经, 骨髓间充质干细胞, 富血小板凝胶, 股神经损伤, 周围神经损伤, 兔

Abstract: BACKGROUND: The use of allogeneic acellular nerve scaffold combined with bone marrow mesenchymal stem cells and platelet-rich gel to treat rabbit sciatic nerve injury is currently less reported and the mechanism is less clear.
OBJECTIVE: To evaluate the effect of transplantation of acellular nerve scaffold combined with bone marrow mesenchymal stem cells and platelet-rich gel in the rabbit sciatic nerve injury model.
METHODS:  Twenty-five New Zealand rabbits were randomly divided into five groups: stump anastomosis group, acellular nerve scaffold group, acellular nerve scaffold combined with platelet-rich gel group, acellular nerve scaffold combined with bone marrow mesenchymal stem cell group, acellular nerve scaffold combined with platelet-rich plasma gel and bone marrow mesenchymal stem cell group. At 12 weeks after operation, the wet weight of gastrocnemius and histopathological staining experiments were performed.  
RESULTS AND CONCLUSION: The wet weight of gastrocnemius in the acellular nerve scaffold combined with platelet-rich gel and bone marrow mesenchymal stem cell group was significantly higher than that in the other groups (P < 0.05); moreover, there were many regenerated nerve fibers, no inflammatory cell infiltration, and positive expression of neurofilament protein NF-200. It is indicated that the combination of acellular nerve scaffold and bone marrow mesenchymal stem cells and platelet-rich gel has a good effect on the regeneration of peripheral nerve injury. 

Key words: stem cells, acellular nerve, bone marrow mesenchymal stem cells, platelet-rich gel, femoral nerve injury, peripheral nerve injury, rabbit

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