中国组织工程研究

中国组织工程研究 ›› 2015, Vol. 19 ›› Issue (20): 3183-3187.doi: 10.3969/j.issn.2095-4344.2015.20.013

• 口腔组织构建 oral tissue construction • 上一篇    下一篇

骨形态发生蛋白2与4在生长发育高峰期骨性Ⅱ类错牙合畸形中的表达

汪  杰，马  策，王豫蓉，陈德余   

  1. 重庆医科大学附属口腔医院正畸科，重庆市高校市级口腔生物医学工程重点实验室，重庆市 401147
  • 出版日期:2015-05-14 发布日期:2015-05-14
  • 通讯作者: 王豫蓉，主任医师，副教授，重庆医科大学附属口腔医院正畸科，重庆市 401147
  • 作者简介:汪杰，女，1989年生，重庆市垫江县人，汉族，重庆医科大学口腔正畸学在读硕士，医师，主要从事下颌骨发育异常导致的错牙合畸形相关分子生物学研究。
  • 基金资助:

    重庆市卫生局重点项目(2009-1-32)

Expression of bone morphogenetic protein-2 and bone morphogenetic protein-4 in Skeletal Class II Malocclusion during growth peak

Wang Jie, Ma Ce, Wang Yu-rong, Chen De-yu   

  1. Department of Orthodontics, the Stomatological Hospital of Chongqing Medical University, Chongqing 401147, China
  • Online:2015-05-14 Published:2015-05-14
  • Contact: Wang Yu-rong, Chief physician, Associate professor, Department of Orthodontics, the Stomatological Hospital of Chongqing Medical University, Chongqing 401147, China
  • About author:Wang Jie, Studying for master’s degree, Physician, Department of Orthodontics, the Stomatological Hospital of Chongqing Medical University, Chongqing 401147, China
  • Supported by:

    the Key Project of Health Bureau in Chongqing, No. 2009-1-32

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被引次数

1

摘要：

背景：课题组以往研究证实，骨形态发生蛋白4对生长发育期下颌骨的生长有促进作用，而骨形态发生蛋白2是否能与骨形态发生蛋白4相互促进下颌骨生长目前未见有相关报道。
目的：检测生长发育高峰期骨性Ⅱ类错牙合畸形患者血液中骨形态发生蛋白2和骨形态发生蛋白4的表达情况，以探究骨形态发生蛋白2和骨形态发生蛋白4的表达量与下颌骨生长的关系。
方法：生长发育高峰期骨性Ⅰ类错牙合畸形患者为Ⅰ组，以下颌后缩为主的骨性Ⅱ类错牙合畸形患者为Ⅱ组，每组18人。采用实时荧光定量PCR(RT-PCR)分别检测两组血液骨形态发生蛋白2和骨形态发生蛋白4的表达。
结果与结论：骨性Ⅱ类错牙合畸形组中骨形态发生蛋白2 mRNA的表达量明显低于对照组骨性Ⅰ类错牙合畸形组(P < 0.05)，骨性Ⅱ类错牙合畸形组中骨形态发生蛋白4 mRNA的表达量明显低于对照组骨性Ⅰ类错牙合畸形组 (P < 0.05)，骨性Ⅱ组中骨形态发生蛋白2与骨形态发生蛋白4的表达有显著相关性。结果证实，骨形态发生蛋白2和骨形态发生蛋白4在生长发育高峰期的表达量均降低与下颌骨发育不足有密切关系，且骨形态发生蛋白2和骨形态发生蛋白4相互协同，共同参与调节下颌骨的生长。

关键词: 组织构建, 骨组织工程, 骨性Ⅱ类错牙合畸形, 下颌后缩, 骨形态发生蛋白2, 骨形态发生蛋白4, 下颌骨, 麦氏软骨, 髁突, 生长发育高峰, 荧光定量PCR, 遗传

Abstract:

BACKGROUND: Preliminary studies of our research group have confirmed that bone morphogenetic protein-4 can stimulate the development of mandible in the growth period, but whether bone morphogenetic protein-4 can interact with bone morphogenetic protein-2 to promote the growth of mandible has not been reported.
OBJECTIVE: To detect the expression of bone morphogenetic protein-2 and bone morphogenetic protein-4 in skeletal class II malocclusion during growth peak, and to explore the relationship of the expression of bone morphogenetic protein-2 and bone morphogenetic protein-4 with mandibular growth.
METHODS: Patients with skeletal class I malocclusion in growth peak served as group I, and those with skeletal class II malocclusion in growth peak characterized as mandibular retrognathia acted as group II. There were 18 cases in each group. Expression of bone morphogenetic protein-2 and bone morphogenetic protein-4 in serum was detected by real-time fluorescent quantitative PCR.
RESULTS AND CONCLUSION: The mRNA expression of bone morphogenetic protein-2 and bone morphogenetic protein-4 in the group II was significantly lower than that in the group I (P < 0.05). In the group II, there was a significant correlation between the expression of bone morphogenetic protein-2 and bone morphogenetic protein-4. These experimental findings confirm that the reduced expression of bone 
morphogenetic protein-2 and bone morphogenetic protein-4 in skeletal class II malocclusion during growth peak has a certain relationship with mandibular deficiency, and moreover, bone morphogenetic protein-2 interacts with bone morphogenetic protein-4 to promote the growth of mandible.

Key words: Malocclusion, Angle Class II, Bone Morphogenetic Proteins, Bone Morphogenetic Protein 4

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