Table of Content

21 October 2012, Volume 16 Issue 43 Previous Issue    Next Issue

For Selected:

Download Citations EndNote Reference Manager ProCite BibTeX RefWorks

Toggle Thumbnails

Select

Promotive effect of injectable thermo-sensitive icariin chitosan hydrogel on radial fracture healing in rabbits Wang De-yin, Rui Gang, Li Pian, Feng Zu-de, Feng Jin-yi 2012, 16 (43):  7981-7985.  doi: 10.3969/j.issn.2095-4344.2012.43.001 Abstract ( 352 )   PDF (578KB) ( 462 )   Save BACKGROUND: Epimedium has in vitro promotive effects on osteoblast osteogenesis, differentiation and maturation. Besides, it can stimulate bone formation. OBJECTIVE: To observe the repair effect of injectable thermo-sensitive icariin chitosan hydrogel on radial fracture healing in rabbits. METHODS: The left and right radius was cut off from 36 rabbits under anesthesia and randomly divided into experimental group, control group and blank group. The rabbits in the former two groups were injected with injectable thermo-sensitive icariin chitosan hydrogel and chitosan hydrogel around the fracture ends, respectively. The rabbits in the blank group had no treatment. RESULTS AND CONCLUSION: Fractures in each group were cured at week 6 after surgery. X-ray films showed that average gray value per unit area and osteoblast count of the experimental group were significantly higher than those of the control and blank groups (P < 05). Moreover, the osteoblast proliferation was active in the experimental group. These results suggest that injectable thermo-sensitive icariin chitosan hydrogel can improve osteoblast activity and promote the fracture healing. Related Articles | Metrics

Select

Compound bone morphogenetic protein-alpha-cyanoacrylate biological glue repairs mandibular defects in rats Wang Xin, Xie Fu-qiang,Zhang Yun, Sun Jian 2012, 16 (43):  7986-7990.  doi: 10.3969/j.issn.2095-4344.2012.43.002 Abstract ( 300 )   PDF (590KB) ( 550 )   Save BACKGROUND: Bone morphogenetic protein 2 is the strongest in the family of bone morphogenetic proteins which can induce osteogenesis separately. OBJECTIVE: To evaluate the effectiveness and feasibility of implantation of the compound bone morphogenetic protein- cyanoacrylate biological glue in the reconstruction of rat mandibular defects. METHODS: Totally 54 male Wistar rats were divided into three groups randomly and equally: The experimental group, the control group (the chitosan group) and the blank group. Mandibular defects of rats were repairing by implanting the compound bone morphogenetic protein-α-cyanoacrylate biological glue in the experimental group. While mandibular defects of the chitosan group were repaired only with chitosan. The blank group received no implantation. RESULTS AND CONCLUSION:①X-ray examination: At weeks 3, 6, 9 postoperation, the area of residual mandibular defects in the experimental group was smaller than that of the other two groups. There was a statistically significant difference between the experimental and the other two groups (P < 0.05). ②Histopathology observation: The new bone mass in the experimental group was superior to those in the other two groups. It is demonstrated bone morphogenetic protein α-cyanoacrylate biological glue can be used to restoration of rat mandibular bone defects and significantly promote the mandibular repair. Related Articles | Metrics

Select

Expression of type Ⅰ collagen in the new-type implant-bone interface He Yun,Chen Jun-liang, Liu Fu-xiang, Gu Ya-lan 2012, 16 (43):  7991-7994.  doi: 10.3969/j.issn.2095-4344.2012.43.003 Abstract ( 450 )   PDF (410KB) ( 399 )   Save BACKGROUND: The new type implant designed by Professor Liu integrates the character of cylinder implant and blade implant. It is shorter but bigger in contact area. The design of new type implant is considered the torsional capacity, lateral force resisting ability, the match of implant and bone elastic modulus, as well as processing and implantation and repair convenience. OBJECTIVE: To investigate the changes of type Ⅰ collagen expression in the implant-bone interfaces after implanted to the jawbone, and to explore the combining capacity of the new type implant and alveolar bone. METHODS: All the premolars in the bilateral jaw of six adult dogs were extracted. Three months later,two new type implants and two cylinder implants were implanted to the sockets and divided to the new type implants group and cylinder implants group. RESULTS AND CONCLUSION: The density of the guided bone tissue in the two groups was increased with time. The tissues with gradually increased density in the disk structure of the new type implant group were found. The expression of type Ⅰ collagen was found in the implant-bone interfaces of the two groups, and both reached the peak at week 4 after surgery. The mean gray value of the new type implant group at weeks 2 and 4 after surgery was higher than that of the cylinder implants group (P < 0.05). These findings suggest that new type implant can achieve success after implanted into the jaw bone due to its special design. The osseointegration of the new type implant is earlier, more active and stronger combining capacity than that of the cylinder implant. Related Articles | Metrics

Select

Proliferation of lymphocytes co-cultured with xenograft cortical bone meal in vitro Tan Xin-yu, Zhang Hui-bi, Lin Mao-qun, Zhang Ying, Tang Xian-gao 2012, 16 (43):  7995-7999.  doi: 10.3969/j.issn.2095-4344.2012.43.004 Abstract ( 354 )   PDF (468KB) ( 577 )   Save BACKGROUND: Xenograft bone materials have been limited in clinical application, mainly due to their strong immunogenicity, but immunogenicity evaluation on the xenograft bone materials is based on the clinical observation or through indirect determination by histopathological observation. Up to now, there is no quantitative indicator or industry "gold standard". OBJECTIVE: To observe the in vitro activation and proliferation of peripheral blood lymphocytes during co-culturing with bone material and to prepare for the next specific immunogenicity experiments. METHODS: The hydroxyapatite (2 g/mL) was selected as negative control, the phytohemagglutinin was considered as positive control. The CCK-8 method was used to detect the effect of 0.5, 1 and 2 g/mL cortical bone meal (treated with degreasing, acellular and multi-faceted antigen processing) and 1 g/mL disinfected cortical bone meal on the proliferation of lymphocytes; flow cytometry was used to detect the effect of CD69 labeled bone materials on the in vitro activation of lymphocytes. RESULTS AND CONCLUSION: There was no significant difference of proliferation of lymphocytes between 0.5, 1 and 2 g/mL cortical bone meal groups and hydroxyapatite group (P > 0.05), while the proliferation of lymphocytes in disinfected cortical bone meal group and phytohemagglutinin positive group was higher than that in the hydroxyapatite group (P < 0.05, 0.01). The low expression rate of CD69 of lymphocytes in the hydroxyapatite group indicated that the T cells were in the static status; there was no significant difference in expression rate of CD69 of lymphocytes between 0.5, 1 and 2 g/mL cortical bone meal groups and hydroxyapatite group (P > 0.05); but the expression rate of CD69 of lymphocytes co-cultured with disinfected cortical bone meal was significantly increased; the expression of CD69 of lymphocytes in the phytohemagglutinin positive group was most obvious. The processed bone meal has no immunogenic effect on lymphocytes. Related Articles | Metrics

Select

Percutaneous kyphoplasty with bone cement treats osteoporotic thoracolumbar vertebral compressive fractures Li Zhi-jun, Ci Yuan, Zheng Yu-peng, Zhang Guang-yu 2012, 16 (43):  8000-8004.  doi: 10.3969/j.issn.2095-4344.2012.43.005 Abstract ( 341 )   PDF (457KB) ( 634 )   Save BACKGROUND: Percutaneous kyphoplasty can recover the vertebral height after compressive fractures through extension of the sacculus. Bone cement is injected in order to strengthen the vertebral bodies, and recover anatomic form and mechanical property. OBJECTIVE: To evaluate the efficacy and safety of percutaneous kyphoplasty in treating osteoporotic thoracolumbar vertebral compressive fractures. METHODS: Totally 41 cases of osteoporotic thoracolumbar vertebral compressive fractures were treated by percutaneous kyphoplasty with injection of polymethylmethacrylate under “C” armed fluoroscopy image guiding. The clinical effect was evaluated by observing the changes of visual analog scale (VAS) and imaging. RESULTS AND CONCLUSION: All cases were treated successfully without any complication. During the 3-month follow-up after operation, the mean height of the anterior and media vertebral body and the Cobb’s angle were improved obviously after operation (P < 0.05), and VAS was decreased at the same time (P < 0.01). It can conclude that the percutaneous kyphoplasty is simple and safe in treating osteoporotic thoracolumbar vertebral compressive fractures, which can fast relieve pain, and improve patient’s quality of life. Related Articles | Metrics

Select

Preparation and properties of calcium polyphosphate fiber/hydroxyapatite/gelatin scaffolds for cartilage tissue engineering Wang Yan-ping, Zhu Ling-yun, Zhang Hong-mei 2012, 16 (43):  8005-8008.  doi: 10.3969/j.issn.2095-4344.2012.43.006 Abstract ( 277 )   PDF (488KB) ( 736 )   Save BACKGROUND: Nowadays, the gelatin scaffold for tissue engineering has many disadvantages, such as low stiffness and strength, bad biocompatibility and uncontrollable degradation rate. OBJECTIVE: To improve the properties of gelatin scaffold by adding calcium polyphosphate fibers and hydroxyapatite. METHODS: Calcium polyphosphate fiber and hydroxyapatite were taken as reinforced materials and gelatin was used as matrix materials, besides, glutaric dialdehyde was used as the cross linker in a ratio of 50/10/40. The composite scaffolds for cartilage tissue engineering were prepared by solvent-casting and particulate-leaching technique, and then their physical and mechanical properties as well as microstructure were tested. RESULTS AND CONCLUSION: The hydroxyapatite powder prepared by sol-gel method had a low crystallization degree, but it had a high crystallization degree after sintered at 900 ℃ for 0.5 hour. The scaffolds with porosity of 65%-90% possessed three-dimensional, interconnecting, micro-hole network structure, and can meet the porosity require of cartilage tissue engineering for scaffolds. The effect of glutaraldehyde cross-linking and improvement of calcium polyphosphate fiber can overcome the disadvantage of easily shrinking of gelatin when the porous scaffold scaffolds are prepared, and provide promise to prepare a high-porosity scaffold material which has three-dimensional and interconnection structure. Related Articles | Metrics

Select

Antimicrobial activity of nano-hydroxyapatite composite root canal filling materials with different pH values in vitro Wang Jian-ping, Wang Yun-feng, Feng Yao, Yue Chang-jun, Dong Bo 2012, 16 (43):  8009-8012.  doi: 10.3969/j.issn.2095-4344.2012.43.007 Abstract ( 392 )   PDF (370KB) ( 455 )   Save BACKGROUND: The antimicrobial activity of root canal filling materials in clinical application is limited at present. Root canal filling materials have detects, such as strong stimulation to tissues, difficulty in formation, bad root canal sealing and easy to be degradable. OBJECTIVE: To study the effect of antimicrobial activity of nano-hydroxyapatite composite root canal filling materials with different pH values on common bacteria infected root canal in vitro. METHODS: In vitro infected root canal model of enterococcus faecalis and candida albicans was established. The antibacterial property of nano-hydroxyapatite paste with different pH values in root canal in vitro was evaluated, including pH 8, pH 9 and pH 10. Zinc oxide eugenol were as a control. RESULTS AND CONCLUSION: All the root canal filling materials have bacteriostatic effect. Compared with the control group, the inhibiting effect of nano-hydroxyapatite paste in each pH value group on enterococcus faecalis was better than that in the control group (P < 0.05). There was no significant difference among nano-hydroxyapatite paste in each pH value group (P > 0.05). The inhibitory effect of nano-hydroxyapatite paste in each pH value group on candida albicans was not better than that in the control group (P < 0.05). Nano-hydroxya paste in each pH value group was compared, and pH 10 group > pH 9 group > pH 8 group (P < 0.05). These findings suggest that in the model of infected root canal, the inhibiting effect of nano hydroxyapatite paste on enterococcus faecalis was better than that of zinc oxide eugenol (P < 0.05), but the inhibiting effect of nano-hydroxyapatite paste on candida albicans was not better than that of zinc oxide eugenol (P < 0.05). Besides, nano-hydroxyapatite composite root canal filling materials with pH=10 have the best bacteriostatic property. Related Articles | Metrics

Select

Effects of “iron nutrient in nanometer” supplementation on hepatic hepcidin mRNA expression after strenuous exercise in rats Pan Xiao-ling, Liao Chun-hai, Pang Yang-kang, Yang Xiao-ping 2012, 16 (43):  8013-8017.  doi: 10.3969/j.issn.2095-4344.2012.43.008 Abstract ( 290 )   PDF (466KB) ( 443 )   Save BACKGROUND: It is a focus that how to improve the benefit of iron supplement in the human body at high intensity exercise, remain body iron homeostasis and increase hemoglobin level. OBJECTIVE: To investigate the effects of “iron nutrient in nanometer” supplementation on the expression of hepatic hepcidin mRNA after high intensity exercise in rats. METHODS: Totally 60 healthy male SD rats were randomly divided into four groups: no-exercise group A (were fed with placebo, n=15), exercise group B (were fed with placebo, n=15), no-exercise group C (“iron nutrient in nanometer” supplementation with lavage method, n=15) and exercise group D (“iron nutrient in nanometer” supplementation with lavage method, n=15). RESULTS AND CONCLUSION: Compared with group A, the expression of hepatic hepcidin mRNA in rats was increased, but the iron concentration was decreased, and total iron binding capacity in serum was increased; transferring saturation was reduced and the concentration of hemoglobin was decreased in group B. But The expression of hepatic hepcidin mRNA was reduced in group C and group D, and the concentration of serum iron increased, total iron binding capacity in serum, and transferring saturation were increased in group C and group D; the concentration of hemoglobin was decreased in group C and group D, but the decreasing degree in group C was lower than that in group D. These findings suggest that “iron nutrient in nanometer” supplementation can significantly inhibit the expression of hepatic hepcidin mRNA after high intensity load exercise in rats, and increase the absorption for “iron nutrient in nanometer” supplementation; besides, it can improve the level of serum iron and hemoglobin and relieve the decreasing degree of exercise-induced hemoglobin. Related Articles | Metrics

Select

Preparation of cyclosporine A nano-particle eye drops and its irritation to the eyes Zhang Hai-juan, Xu Qing, Ma Ke, Wu Yan, You Yu-xia 2012, 16 (43):  8018-8022.  doi: 10.3969/j.issn.2095-4344.2012.43.009 Abstract ( 397 )   PDF (508KB) ( 434 )   Save BACKGROUND: Cyclosporine A can inhibit the immunological rejection after corneal transplantation. But it cannot be formulated into ophthalmic solution for its insolubility. OBJECTIVE: To prepare 1% cyclosporine A nano-particle eye drops and to investigate its irritation to the eyes. METHODS: Cyclosporine A was successfully incorporated into poly (ethylene glycol)-poly (dl-lactide) (PEG-PLA), and 1% cyclosporine A nano-particle eye drops were prepared. The nano-particle eye drop was characterized by using transmission electron microscopy and dynamic light scattering. The irritation of cyclosporine A nano-particle eye drops was observed through single and multiple irritative tests to the eyes in rabbits. RESULTS AND CONCLUSION: Drug-loading and encapsulation efficiency of cyclosporine A nano-particle eye drops were 21.3% and 85.10%, respectively. The cyclosporine A nano-particles showed sphericity with even dispersion. The average size of cyclosporine A-loaded nano-particle was (296.9±32.06) nm with narrow distribution. Drug release curve in vitro exhibited a sustained drug release of cyclosporine A from the nano-particles. The release amount was 91.3% of cyclosporine A around 20 days. The irritation scores on the conjunctiva, cornea and iris were zero at the period of single and multiple administrations, indicating no irritation was found. These findings suggest that the cyclosporine A nano-particle has no irritation to the eyes. Related Articles | Metrics

Select

Preparation and characterization of nano-silver slow-release chitosan/polyvinyl alcohol sponge wound dressings Xu Wei-kang, Wei Kun, Zeng Xiao-feng, Ling You, Zhang Jie, Wei Xin-miao 2012, 16 (43):  8023-8026.  doi: 10.3969/j.issn.2095-4344.2012.43.010 Abstract ( 562 )   PDF (461KB) ( 661 )   Save BACKGROUND: The clinical treatment of traditional wound infection is mainly use antibiotic drugs dressings, but the long-term use can cause bacteria resistance. Meanwhile, nano wound dressings used in clinical application has poor biocompatibility and cannot be degraded, which should not be used for long-term coverage of wound. OBJECTIVE: To prepare a wound dressing with antibacterial action and good biocompatibility, and to characterize and analysis its biological properties. METHODS: Chitosan/polyvinyl alcohol sponge containing nano-silver/polyethyleneglycol was prepared by acetalization reaction. The material’s mechanical properties, surface topography, in vitro release, and antibacterial properties were detected. RESULTS AND CONCLUSION: The particle diameter of nano-silver prepared was small and it had good disparity. The in vitro release studies showed that Ag nano-silver particles were able to release continuously and sterilize the bacterium. The Chitosan/polyvinyl alcohol sponge containing nano-silver/polyethyleneglycol had dense and uniform interspaces; the pores inside was interconnected, and had favorable moisture-adsorption rate, moisture-retention rate, and moderate air permeability rate. The moisture-adsorption rate and air permeability rate were increased with the content of polyvinyl alcohol 1799 increased, but the moisture-retention rate almost had no effects with the changes of the content of polyvinyl alcohol 1799. The material had a good bactericidal effect on the staphylococcus aureus, escherichia coli, candida albicans, pseudomonas aeruginosa and salmonella typhi. These findings suggest that the dressing not only possesses good physical properties and anti-microbial, but also its synthesis procedure is simple and low cost. Therefore, the developed material can be applied to new wound dressing. Related Articles | Metrics

Select

Porous tantalum scaffold compounded with synovium versus tricalcium phosphate compounded with synovium for repairing cartilage defects Liu Ning, Qian Qi-rong, Xu Wei, Peng Jin-hui, Zhou Rong 2012, 16 (43):  8027-8031.  doi: 10.3969/j.issn.2095-4344.2012.43.011 Abstract ( 297 )   PDF (522KB) ( 440 )   Save BACKGROUND: Early experiments have proved that synovium contains mesenchymal stem cells which can potentially form the cartilage or bone and differentiate into chondrocytes under property conditions. OBJECTIVE: To compare the repair effect of porous tantalum combined with synovium and tricalcium phosphate combined with synovium on cartilage defects. METHODS: Totally 24 female New Zealand rabbits were randomly divided into experimental group (a porous tantalum scaffold combined with synovium) and control group (a tricalcium phosphate scaffold combined with synovium). A model of cartilage defect in the knee joint was establishe, and then the two kinds of scaffolds were implanted. A piece of pretreated inverse synovium was covered on the surface of defects, and plaster fixation was performed for 2 weeks. The rabbits were executed under anesthesia at week 12. The gross observation of the synovium, joint fluid and femoral condylar cartilage, as well as the pathological features of the femoral condylar cartilage was observed. Improved Mankin score was obtained to evaluate the repair effect. RESULTS AND CONCLUSION: In the experimental group, there was various synovial hyperplasia, and the surface of new cartilage was blue and white, and the margin was less smooth. Toluidine blue staining showed that chondrocytes arranged a little disorder. Resides, the number of chondrocytes was normal, moreover the porous tantalum scaffolds were fully filled with bone tissues, and the Mankin score was 7.35. In the control group, the surface of new cartilage was blue and white, and the margin was less smooth. Toluidine blue staining showed that chondrocytes arranged a little disorderly. The number of chondrocytes was normal. In addition, the tricalcium phosphate scaffolds were partly filled with bone, and the Mankin score was 7.43 (P > 0.05). These findings suggest that there is no significant difference between porous tantalum combined with synovium and tricalcium phosphate combined with synovium in repairing cartilage defects, but the fusion of porous tantalum scaffolds with surrounding bone tissues is superior to that of tricalcium phosphate. Related Articles | Metrics

Select

Coralline hydroxyapatite combined with guided bone regeneration for repairing alveolar bone defects Dai Xiao-wei, Ruan Xiao-hui, Nijiati·Tuerxun 2012, 16 (43):  8032-8036.  doi: 10.3969/j.issn.2095-4344.2012.43.012 Abstract ( 428 )   PDF (468KB) ( 691 )   Save BACKGROUND: The coralline hydroxyapatite is take high quality natural narine biological as raw materials. It forms hydroxyapatite thin layer in coral skeleton, so it keeps natural coral distributed framework, thereby providing a good space for tissue growth. OBJECTIVE: To investigate the clinical effect of coralline hydroxyapatite on guiding bone regeneration for the repair of alveolar bone defects. METHODS: Forty-two patients with bone defects caused by mandibular first molar periodontitis were collected and randomly divided into experimental group and control group. Patients in the experimental group were treated with coralline hydroxyapatite combined with oral biofilm to fill and repair bone defects, and those in the control group were only treated with coralline hydroxyapatite. RESULTS AND CONCLUSION: The patients were followed up for 12 months and the results showed that periodontal attachment loss was found in the two groups, besides, periodontal probing depth was improved obviously after treatment (P < 0.05). In addition, compared with the control group, periodontal attachment loss and periodontal probing depth in the experimental group were better (P < 0.05). Moreover, new bone formation density and bone mass of the experimental group were superior to those of the control group (P < 0.05). These results suggest that coralline hydroxyapatite combined with guided bone regeneration technique can have good effect on guiding bone regeneration and repair bone defects. Related Articles | Metrics

Select

Effect of polyurethane binder with catalyst on the enamel-porcelain adhesive property Chen Xiang-tao, Wang Zhi-xia, Chen Lin, Yue Chang-jun, Wang Jia-qi 2012, 16 (43):  8037-8040.  doi: 10.3969/j.issn.2095-4344.2012.43.013 Abstract ( 303 )   PDF (514KB) ( 447 )   Save BACKGROUND: Although polyurethane binder has a strong cohesive force, the cohre conditions do not comply with the basic conditions used in oral adhesive. OBJECTIVE: To observe the effect of catalyst on the performance of polyurethane binder. METHODS: Freshly extracted 20 non-carious human premolars or anterior teeth were collected to prepare flat mesial and distal enamel bonding interface with diamond bur. Totally 40 ceramic discs (3 mm in diameter, 3 mm in height) were fabricated, and then cemented on enamel bonding interface. These teeth and ceramic discs were evenly divided into four groups: stannous octoate binder group (polyurethane binder+stannous octoate), pentamethyldie thylenetriamine group (polyurethane binder+pentamethyldie thylenetriamine), the pure polyurethane binder group and Panaviya F adhesive group. RESULTS AND CONCLUSION: Adhesive strength of the stannous octoate binder group was the biggest, and that of the pentamethyldie thylenetriamine and pure polyurethane binder groups were smaller, while that of the Panaviya F adhesive group was the minimum. Adhesive strength of stannous octoate binder group, pentamethyldie thylenetriamine group and pure polyurethane binder group were all higher than that of the Panaviya F adhesive group (P < 0.05). Besides, the addition of stannous octoate and pentamethyldie thylenetriamine group shortened the solidification time. These findings suggest that catalyst can effectively shorten the solidification time of the polyurethane binder, and improve the adhesive strength of the polyurethane binder. Related Articles | Metrics

Select

Effects of titanium nitride coating and bond porcelain on the bonding strength of titanium porcelain Liu Jie, Shi Lei, Fan Jin-jie, Ge Ya-li, Gu Xiao-ming 2012, 16 (43):  8041-8044.  doi: 10.3969/j.issn.2095-4344.2012.43.014 Abstract ( 454 )   PDF (560KB) ( 416 )   Save BACKGROUND: Titanium nitride (TiN) coating and bond porcelain can improve the bonding strength of titanium porcelain. OBJECTIVE: To investigate the influence of TiN and bond porcelain on the bonding strength of titanium porcelain． METHODS: Totally 32 cast pure Ti plates were prepared. After sandblasting, the cast plates were divided into four groups: A, B, C and D groups. A: pure Ti group, B: TiN coating group, C: bond porcelain coating group, D: TiN+bond porcelain coating group. And then porcelain was baked at the center of the plates. All plates were submitted to a three-point bending test. RESULTS AND CONCLUSION: The titanium-porcelain binding stength of the group A was lower than that of the groups B, C and D (P < 0.05).There were no significant differences in the bonding strength among the groups B, C and D (P > 0.05). Titanium porcelain interfaces of groups B, C and D were linked closely together, and there were no obvious pores and bubbles. These findings suggest that the application of the TiN and bond porcelain is helpful to improve the bonding strength of titanium porcelain. If they are handled in two ways simultaneously, the bonding strength of titanium porcelain cannot be improved better. Related Articles | Metrics

Select

Effects of noble ceramic alloy and high glucose on inflammatory factors in the extracellular fluid Xie Hui, Ding Jiang-feng 2012, 16 (43):  8045-8048.  doi: 10.3969/j.issn.2095-4344.2012.43.015 Abstract ( 481 )   PDF (396KB) ( 416 )   Save BACKGROUND: The preliminary studies have found that there is an interaction effect of high sugar and ceramic alloy extracts on proliferation of L-929 mouse fibroblasts. OBJECTIVE: To observe the effects of three kinds of noble ceramic alloys and high glucose on secrets of interleukin-6 from L-929 cells. METHODS: Using two-factor factorial design experiments, we detected the changes of interleukin-6 levels in the extracellular fluid after co-culture of 89% Au-alloy, 74% Au-alloy and Ag-Pd alloy with mouse L-929 cells under different glucose concentrations (11.1, 22.2, 33.3 mmol/L). Cells without alloy served as negative controls. RESULTS AND CONCLUSION: Two-factor factorial design results showed that the main effect of noble ceramic alloy extracts was statistically significant (P=0.001). The interleukin-6 levels of the supernatant in the 74% Au-alloy and the Ag-Pd alloy were significantly lower than that in the negative group (P=0.004, 0). The main effect of glucose concentration was also of statistical significance. The interleukin-6 level in supernatant in 33.3 mmol/L high glucose was significantly lower than that in 11.1 mmol/L glucose. However, no interaction existed between different extracts of noble ceramic alloys and glucose concentrations about the secrets of interleukin-6 from L-929 cells (P=0.33). Related Articles | Metrics

Select

Cytocompatibility of the scaffold materials in vascular tissue engineering Xiao Rong-dong, Weng Guo-xing 2012, 16 (43):  8049-8054.  doi: 10.3969/j.issn.2095-4344.2012.43.016 Abstract ( 414 )   PDF (585KB) ( 429 )   Save BACKGROUND: Collagen and hyaluronic sacid are both helpful to cell adhesion, proliferation and differentiation in the tissue culture. OBJECTIVE: To investigate the cytocompatibility between the three scaffolds (collagen, hyaluronic acid membrane, and latin sponge) and vascular endothelial cells, smooth muscle cells, as well as to screen the optimal seeding method. METHODS: The passage 3-5 vascular smooth muscle cells of rabbits were seeded onto the collagen/hyaluronic acid membrane (or latin sponge); after cultured for 2 weeks, rabbit vascular endothelial cells were seeded onto the compound of smooth muscle cells-collagen/hyaluronic acid membrane (or latin sponge). Pure endothelial cells and smooth muscle cells were performed inoculation together. RESULTS AND CONCLUSION: ①Light microscope and scanning electron microscope results showed that the inoculation times and growth were increased with culture time, and the cells on the collagen/hyaluronic acid membrane grew better, what’s more, cells attached more tightly. ②WST-1 method results showed that the adhesion rate and proliferation rate of smooth muscle cells in the collagen/hyaluronic acid membrane group were both higher than the latin sponge group (P < 0.05). Besides, cells growth on the scaffolds was increased in various degrees. ③3H-TDR method results indicated that DNA synthesis of cells in the collagen/hyaluronic acid membrane group was highest, and that in the latin sponge group was lower. These findings suggest that collagen/hyaluronic acid membrane has ideal cytocompatibility. Repeating seeding with proper interval can increase the adhesion and proliferation of cells on scaffolds. Related Articles | Metrics

Select

In vitro cellular biocompatibility of konjac glucomannan-hyaluronic acid blend membrane Gu Shao, Pan Xian-feng, Xu Yong-qing, Chen Jia, Chen Qing-hua 2012, 16 (43):  8055-8060.  doi: 10.3969/j.issn.2095-4344.2012.43.017 Abstract ( 327 )   PDF (605KB) ( 485 )   Save BACKGROUND: Hyaluronic acid and konjac glucomannan can be used for the prevention of adhesion after surgery. OBJECTIVE: To investigate the biocompatibility of konjac glucomannan-hyaluronic acid blend membrane co-cultured with bone marrow mesenchymal stem cells (BMSCs) of rabbits. METHODS: The passage 3 BMSCs were cultured with konjac glucomannan-hyaluronic acid blend membrane in vitro. Their compound degree was observed by inverted microscope and scanning electron microscopy. Cell proliferation was detected by MTT assay. The BMSCs on the konjac glucomannan-hyaluronic acid blend membrane were orientiatedly induced into adipocytes in vitro. Differentiation effect was detected by oil red O staining RESULTS AND CONCLUSION: BMSCs were well cultured with konjac glucomannan-hyaluronic acid blend membrane. Good adhesion and proliferation was found under inverted microscope and scanning electron microscope. The BMSCs on the konjac glucomannan-hyaluronic acid blend membrane could be orientiatedly induced into adipocytes, and had odipogenic potential. These findings suggest that there is a good biocompatibility between BMSCs and the konjac glucomannan-hyaluronic acid blend membrane. Related Articles | Metrics

Select

In vivo test of a magnetic suspension centrifugal ventricular assist device and its hemocompatibility Li Hai-yang, Wu Guang-hui, Wang Cheng-de, Lin Chang-yan, Hou Xiao-tong, Chen Chen, Yang Peng, Qu Wen-bo, Liu Xiu-jian, Xu Chuang-ye 2012, 16 (43):  8061-8066.  doi: 10.3969/j.issn.2095-4344.2012.43.018 Abstract ( 370 )   PDF (829KB) ( 675 )   Save BACKGROUND: The unique apex intubation design of a magnetic suspension centrifugal ventricular assist device can finish left ventricular intubation with heart beating, which significantly shortens the operative time and avoids the harmful effects of cardiopulmonary bypass. OBJECTIVE: To verify the in vivo reliability of the magnetic suspension centrifugal left ventricular assist device. METHODS: The magnetic suspension centrifugal left ventricular assist device was implanted into the left ventricular apex of a healthy sheep with heart beating. The outflow graft of each device was anastomosed to the descending aorta. Hematologic, biochemical and blood clotting tests before and after surgery were performed on schedule. Data associated with pump function were collected continuously, and the end-organs were examined macroscopically and histopathologically. RESULTS AND CONCLUSION: During the in vivo assisted 38 days, no mechanical wearing and the thrombus formation at inflow and outflow were observed inside the pump. Hematologic and biochemical test results were within the normal range. There were no significant complications in the sheep. Macroscopical and histopathological examination of the end organs revealed no evidence of ischemia or infarction. These findings suggest that the magnetic suspension centrifugal left ventricular assist device has good in vivo hemocompatibility and reliability. Related Articles | Metrics

Select

Effect of different pore sizes of hollow titanium alloy prosthesis’s wall on the growth of bone marrow stromal cells Fan Yu-ping, Huang Jing-xiang, Cai Xu 2012, 16 (43):  8067-8070.  doi: 10.3969/j.issn.2095-4344.2012.43.019 Abstract ( 241 )   PDF (432KB) ( 440 )   Save BACKGROUND: The growth behavior of cells on a stent is influenced by the pore size and porosity of stents as well as many other factors. OBJECTIVE: To observe the effect of different pore sizes of hollow titanium alloy prosthesis’s wall on the growth of bone marrow stromal cells (BMSCs). METHODS: Hollow porous metal prostheses were divided into two groups by the pore size of the wall, which were φ1: 1 mm and φ2: 1.5 mm. The prosthesis was located in the BMSCs suspension for osteogenic induction. BMSCs without osteogenic induction served as controls. The cell morphology, growth and calcium nodules were observed under inverted phase contrast microscope. RESULTS AND CONCLUSION: At 4 week, BMSCs were linked directly through the hole of the groupφ1, but not in the groupφ2. Calcium nodules were formed in the groupsφ1 andφ2. These indicate that BMSCs cultured in vitro can be linked directly in the proper pore size to prevent a potential gap between implants and the host bone. In addition, this spatial structure can provide sufficient space for the growth of regenerated bones. Related Articles | Metrics

Select

Absorbable magnesium alloy stents in the coronary artery Wang Ping, Lei Li-cheng, Wang Li-li, Wang Ru-peng, Chen Li-li, Cui Yu-xia, Liu Yan, Wang Shuang1, Yang Nuo, Xu Gui-yu, Zhao Nan-nan, Chang Hong, Gao Li, Wang Er-de, Wu Kun, Cui Fu-zhai 2012, 16 (43):  8071-8077.  doi: 10.3969/j.issn.2095-4344.2012.43.020 Abstract ( 399 )   PDF (634KB) ( 605 )   Save BACKGROUND: At present, the subacute thrombosis and restenosis cannot be fundamentally resolved by the local drug-eluting stent which is based on cobalt-chromium alloy in the coronary artery. Therefore, the bio-absorbable stents have become the focus. OBJECTIVE: To evaluate the biocompatibility, effectiveness and safety of absorbable magnesium alloy stents in the coronary artery. METHODS: Totally 35 immunized hybrid dogs were all implanted an absorbable magnesium alloy stent in the coronary artery and/or the femoral artery. Each dog received the coronary and femoral angiography after implantation for 24 hours, 3 days, 5 days, 1 week, 2 weeks, 3 weeks and 4 weeks respectively. And then the stent arteries were isolated for histopathological analysis and computer image analysis, besides, the elastic lamina area, lumen area, and the percentage of intimal hyperplasia area were measured. RESULTS AND CONCLUSION: Fifty-one stents were implanted in 35 dog’s coronary and femoral arteries successfully. The angiography of the coronary and femoral arteries at different time points after implantation was found the clear lumen, as well as no constriction and thrombosis; besides, the stent was completely degraded at 1 week after implantation. The results of histopathological analysis showed that there was a slight intimal hyperplasia at week 2 after implantation, and the percentage of hyperplasia area was increased with time. These findings suggest that the absorbable magnesium alloy stent in the coronary artery can be degraded within 1 week, and no significant inflammatory response and thrombosis formation were found, what’s more, restenosis degree was slight. Therefore, this kind of stent has good biocompatibility, effectiveness and safet Related Articles | Metrics

Select

Electrophysiological changes following transplantation of tissue engineered myocardial tissue constructed by the compound of myocardial cells and collagen materials in rats with myocardial infarction Sun Juan, Chen Xiao-yang, Zhang Ling, Huang Yan 2012, 16 (43):  8078-8082.  doi: 10.3969/j.issn.2095-4344.2012.43.021 Abstract ( 352 )   PDF (497KB) ( 692 )   Save BACKGROUND: Transplantation of tissue engineered myocardial tissue can repair myocardial infarction in rats. OBJECTIVE: To study the electrophysiological characteristics after the transplantation of tissue engineered myocardial tissue. METHODS: Thirty SD rats were evenly divided into three groups: control group, model group and transplantation group. A rat model of myocardial infarction was established in the latter two groups. The control group underwent thoracotomy with no ligation of the coronary artery. The transplantation group was transplanted with the compound of myocardial cells and collagen materials after modeled. Amplitude, activation-recovery interval and activation-conduction duration at local myocardium were recorded with microeletrode arrays technique. RESULTS AND CONCLUSION: ①Compared with the model group, the stationary field amplitude of ventricular myocardium in infarction zone, opposite zone and border zone in the transplantation group were significantly increased (P < 0.05). ②Activation-recovery interval in the model group, as well as infarction zone and infarcted border zone in the transplantation group were prolonged variously (P < 0.05). While the activation-conduction duration of infarction zone, opposite zone and border zone in the transplantation group were shorten compared with the model group. ③In terms of the activation-conduction duration of left ventricular anterior wall, the transplantation group was higher than the control group, but lower than the model group (P < 0.05). These findings suggest that the compound of myocardial cells and collagen materials transplanted can improve the contractile function of the ventricular muscle and electrical conduction time, as well as repair the heart partly. Related Articles | Metrics

Select

In vitro coculture of bone marrow mesenchymal stem cells with the small intestinal submucosa Wei Ren-qian, Cao Xing-hai, Deng Rui, Yang Zhi-ming, Tan Bo, Jin An-min 2012, 16 (43):  8083-8089.  doi: 10.3969/j.issn.2095-4344.2012.43.022 Abstract ( 337 )   PDF (678KB) ( 557 )   Save BACKGROUND: Small intestinal submucosa extracellular matrix is a kind of material used to build the single structural engineering scaffolds with low immune response and good biocompatibility. OBJECTIVE: To observe the biocompatibility of bone marrow mesenchymal stem cells growing on the small intestinal submucosa in vitro. METHODS: The primary bone marrow mesenchymal stem cells were separated and purified by density gradient centrifugation combined with adherent methods. The cells were labeled using the red fluorescent cell linker kit before seeding. The passage 2 labeled bone marrow mesenchymal stem cells were seeded on the small intestinal submucosa. RESULTS AND CONCLUSION: ①Histological observation showed that the bone marrow mesenchymal stem cells formed a single layer on the surface of small intestinal submucosa after coculture for 1 week. The labeled bone marrow mesenchymal stem cells on the scaffold showed uniformly red fluorescence by fluorescence microscope. Two weeks later, the cells were in multi-layer, and displayed intensively red fluorescence. ②Scanning electron microscope showed that, after 2 days of coculture, bone marrow mesenchymal stem cells were adherent to the small intestinal submucosa; 1 week later, the small intestinal submucosa was covered with the collagen secreted by bone marrow mesenchymal stem cells; 2 weeks later, the cells proliferated and fused on co-cultured small intestinal submucosa and closely connected, which showed stratifications and secreted a large number of matrix. The data obtained from the present study shows that bone marrow msenchymal stem cells and small intestinal submucosa have a good compatibility. Related Articles | Metrics

Select

Pathological changes of the corneal epithelial tissue in ophthalmoxerosis rabbits after treatment with chitosan/polyacrylic acid in situ gel Zhang Jie, Wei Kun, Wei Xin-miao, Ling You, Xu Wei-kang 2012, 16 (43):  8090-8093.  doi: 10.3969/j.issn.2095-4344.2012.43.023 Abstract ( 292 )   PDF (516KB) ( 488 )   Save BACKGROUND: Chitosan (CS) has various good properties, such as reproducible, broad sources, easy degradation and excellent biocompatibility, which is a potential raw material for the preparation of in situ forming gel. OBJECTIVE: Investigate the repairing effectiveness of CS/polyacrylic acid (PAA) in situ forming gel on the corneal tissue in rabbits with ocular injury. METHODS: Twenty rabbits were collected to establish rabbit models of ophthalmoxerosis. Fourteen days after surgery, all rabbits were randomly divided into experiential group and control group. The rabbits in the experiential group were treated with CS/PAA in situ forming gel at ocular surface, three times per day. The rabbits in the control group had drug treatment. RESULTS AND CONCLUSION: After treated with the CS/PAA in situ forming gel, the ultrastructure of the corneal epithelium of rabbits was close to normal, and epithelial cells were intact and closely arranged. The defect area was covered with the newborn epithelial cells with regular arrangement, and edema was rare. Meanwhile, the microvilli and micro wrinkles of epithelial cells were increased obviously, indicating the as-prepared preparation was effective for the treatment of ophthalmoxerosis. These findings suggest that the CS/PAA in situ forming gel can repair the corneal epithelial tissue in ophthalmoxerosis. Related Articles | Metrics

Select

Application of Micro-CT combined with epidurography in medical self-crosslinked sodium hyaluronate gel for the prevention of epidural adhesions after laminectomy Liang Wen-kai, Guo Quan-yi, Han Shu-feng, Zhang Li, Peng Jiang, Wang Ai-yuan, Sui Xiang, Liang Zeng-yi, Xu Wen-jing, Lu Shi-bi 2012, 16 (43):  8094-8099.  doi: 10.3969/j.issn.2095-4344.2012.43.024 Abstract ( 319 )   PDF (580KB) ( 588 )   Save BACKGROUND: Medical self-crosslinked sodium hyaluronate gel can effectively prevent epidural adhesions after laminectomy in rabbits within 8 weeks after operation, which has been confirmed by planar angle. OBJECTIVE: To observe and evaluate the practicability of self-crosslinked sodium hyaluronate gel in preventing epidural adhesions by using Micro-CT combined with epidurography. METHODS: Eighteen new Zealand white rabbits with total lumbar laminectomy at L5 level were randomly divided into three groups: control, HyaRegen/SPⅠ and HyaRegen/SPⅡ groups. In the control group, only normal saline was used to flush the laminectomy area before wound closure, while in HyaRegen/SPI and HyaRegen/SPⅡ groups, the exposed dura mater was covered with 0.5 mL HyaRegen/SPI or 0.5 mL HyaRegen/SPII, respectively before wound closure. RESULTS AND CONCLUSION: The latency of cortical somatosensory evoked potentials was not delayed obviously in each group, and there was no significant difference in the three groups (P > 0.05). The scanning of Micro-CT combined with epidurography and the images of three-dimensional reconstruction showed that contrast agent could fill the epidural space smoothly in the HyaRegen/SPⅠ and HyaRegen/SPⅡ groups, but not in the control group, and many filling defects in local operation area could been observed. In addition, the filling volume of contrast agent of the HyaRegen/SPⅠ and HyaRegen/SPⅡ groups in the unit volume of operation area was higher than that in the control group (P < 0.05). There was no significant difference between HyaRegen/SPI and HyaRegen/SPⅡ groups (P > 0.05). Moreover, the degree of epidural adhesions of the control group was significantly higher than that in the HyaRegen/SPI and HyaRegen/SPⅡ groups (P < 0.05). These findings suggest that medical self-crosslinked sodium hyaluronate gel can effectively prevent epidural adhesions after laminectomy in rabbits. Besides, epidural adhesions can be observed and evaluated effectively by using Micro-CT combined with epidurography. Related Articles | Metrics

Select

Silk fibroin as a scaffold material for corneal tissue engineering Ma Rui-jue, Guo Hui-ling, Du Gai-ping, Huang Yi-fei 2012, 16 (43):  8100-8104.  doi: 10.3969/j.issn.2095-4344.2012.43.025 Abstract ( 364 )   PDF (536KB) ( 655 )   Save BACKGROUND: Silk fibroin has the properties of pellucidness, structural plasticity, single component, mechanical strength, toughness and biocompatibility. OBJECTIVE: To review the research progress of silk fibroin as a scaffold material for corneal tissue engineering at home and abroad. METHODS: Databases of PubMed (1980/2011) and CNKI (1990/2011) were selected to retrieve the related articles about silk fibroin and cornea with the keywords of “silk fibroin, corneal, ocular” in English and Chinese, respectively. RESULTS AND CONCLUSION: Owing to the good properties of silk fibroin extracted from nature silk, such as good biocompatibility, special mechanical property, optic transparency and controlled degradation rate, the silk fibroin cannot only be applied in the reconstruction of corneal tissue structure, but also be combined with other tissue materials. The silk fibroin has become an ideal material for corneal tissue engineering. It has been proved that many kinds of corneal cells can grow well on the silk fibroin, and however, there are rare studies about application of cells cultured in vitro in animal models. In addition, there are few data addressing the influence of implantation of silk fibroin into the cornea, which are problems to be solved. Related Articles | Metrics

Select

A new development of silk fibroin scaffolds in tissue engineering Xie Min-kai, Xu Yue-min 2012, 16 (43):  8105-8110.  doi: 10.3969/j.issn.2095-4344.2012.43.026 Abstract ( 310 )   PDF (552KB) ( 608 )   Save BACKGROUND: Silk fibroin scaffolds have been recommended to use in tissue-engineered bone and cartilage, tendon, vessel, nerve, and bladder. OBJECTIVE: To summarize the application and current development of silk fibroin scaffolds in the fields of biomaterials and tissue engineering. METHODS: An online search was conducted by the first author in PubMed database and CNKI database to identify the articles related to silk fibroin scaffold preparation technique, modification of silk fibroin and silk fibroin tissue engineering published from January 2000 to November 2011. RESULTS AND CONCLUSION: Silk fibroin scaffolds has excellent mechanical strength, slow biodegradability, superior biocompatibility and processability. And they can support various cells to attach, differentiate and grow. The scaffolds have been applied in tissue engineering studies, such as artificial ligament, blood vessels, bone, nerve tissue and so on. To date, diverse biologicals, such as growth factors or cytokines, were incorporated therein silk fibroin scaffolds, to further expand the application range in tissue engineering. Related Articles | Metrics

Select

Development of arginine-glycine-aspartic acid peptide analogues in endothelial targeted therapy of neovascularization Zhou Yan-fang, Deng Yu-bin 2012, 16 (43):  8111-8116.  doi: 10.3969/j.issn.2095-4344.2012.43.027 Abstract ( 442 )   PDF (559KB) ( 690 )   Save BACKGROUND: Vascular endothelium plays an important role in ischemia, thrombosis, inflammation, edema, oxidative stress and other pathological damages. It is critical to select the specific endothelial target for drug intervention. OBJECTIVE: To analyze and summarize the researches about arginine-glycine-aspartic acid (RGD) peptide and its analogues used as specific endothelial targets in recent years. METHODS: A computer-based online search of related articles published from January 1998 to December 2011 was performed in PubMed database using the key words of “RGD, integrin, targeted therapy”. The documents related to neovascularization were included. The literatures of irrelevant purpose and repetitive content were excluded. Finally, 42 literatures were chosen to summarize. RESULTS AND CONCLUSION: Integrin αvβ3 is the specific target in pathological damage of endothelial cells and neovascularization which is involved in the migration, proliferation and differentiation of endothelial cells. As the interacting recognition sites of integrin and its ligand, RGD peptide can be combined with integrin αvβ3 with high expression in tumor or pathological angiogenesis, and can mediate the interactions between cell-extracellular matrix and cell and cells. RGD peptide plays an important role in angiogenesis imaging, targeted drug delivery and carrier material modification. Related Articles | Metrics

Select

Application progresses in drug-loaded artificial bone Chen Xiao-le, Yang Qing-song, Su Jia-can 2012, 16 (43):  8117-8121.  doi: 10.3969/j.issn.2095-4344.2012.43.028 Abstract ( 426 )   PDF (516KB) ( 511 )   Save BACKGROUND: Drug delivery system in orthopedics department has become an important method for orthopedic diseases. As a part of it, drug-loaded artificial bone is a newly emerging treatment at present. OBJECTIVE: To review the classification, property, research methods and research direction of drug-loaded artificial bone. METHODS: PubMed and VIP databases were searched by first author (2001/2011) for papers concerning the classification, research methods and application of drug-loaded artificial bone. RESULTS AND CONCLUSION: There are three main types of drug-loaded artificial bones: hydroxylapatite, calcium phosphate cement and bioglass. The former two have gradually applied to clinical research and treatment. Bioglass is a new research material recently, which has special functions as filling bone materials and drug released carriers, but its properties and application are need to further study. The research methods of drug-loaded artificial bone can start with the mechanisms, such as drug carrier hole choice, artificial bone preparation, drug ingredients of drug-loaded artificial bones and drug release of artificial bone. Drug-loaded artificial bones are the drug carrier and can repair bone defects, besides, they can induce the growth and snchronous degradation. They are ideal and effective methods in the application of osteomyelitis, bone defects as well as the prevention of artificial joint infection owing to their easy operation and good effectiveness. Related Articles | Metrics

Select

Characteristics of new-type bone graft materials in scoliosis correction and fusion Hu Wen, Huang Qi-kai, Wei Xian-zhao, Su Jia-can, Li Ming 2012, 16 (43):  8122-8126.  doi: 10.3969/j.issn.2095-4344.2012.43.029 Abstract ( 375 )   PDF (562KB) ( 440 )   Save BACKGROUND: With the advances in surgical techniques, especially the promotion of scoliosis operation, the bone graft material has put forward higher requirements. Bone grafting material should not only have osteogenic activity, but also display similar characteristic on the structure and mechanical properties of natural bone, and meet the biomechanical requirements. OBJECTIVE: To review the application progresses of new-type bone graft materials in scoliosis correction and fusion. METHODS: Elsevier full-text database was retrieved by computer for relevant articles (2009-2012) with the key words of “non-hydroxyapatite, hydroxyapatite, scoliosis” in English. At the same time the HowNet and China Journal Full-text databases were searched for relevant articles with the key words of “scoliosis, bone graft material, nano artificial bone, bone morphogenetic protein 2, artificial bone” in Chinese. Totally 27 articles were included. RESULTS AND CONCLUSION: Nano artificial bone has excellent biocompatibility and bone conduction. It imitates the component and structure characteristic of natural bone. Besides, its structure is basically the same with the natural bone crystals part to provide a microenvironment which is similar to natural bone. In spine surgery, bony fusion is a marker of successful treatment of scoliosis surgery. However, spine surgeons have been puzzled by the source of bone graft materials. So the hot spots at home and abroad are looking for bone repair materials that cannot only form bone fusion but also have osteogenic activity. Nano artificial bone shows a broad prospect in the application of spinal surgery. Related Articles | Metrics

Select

Biomedical application of chitosan-based polyelectrolyte hydrogels Zhang Luan, Huang Xia, Yang Qing-liang, Yang Yang, Shen Chang-yu 2012, 16 (43):  8127-8132.  doi: 10.3969/j.issn.2095-4344.2012.43.030 Abstract ( 499 )   PDF (558KB) ( 847 )   Save BACKGROUND: Chitosan-based polyelectrolyte complexes are prepared on the basis of the property of cationic polymer without using chemical cross-linking agent and under mild conditions. They have good biocompatibility. OBJECTIVE: To review the application of chitosan-based polyelectrolyte in drug carrier, tissue engineering, wound dressing, biosenser and gene carrier. METHODS: ACS database and Elsevier Science electronic journal were searched by the first author for papers concerning the application of chitosan-based polyelectrolyte in bio-medical fields (2004/2011), especially the studies in drug carrier, tissue engineering, wound dressing, biosenser due to its good biocompatibility RESULTS AND CONCLUSION: Chitosan can be applied in drug carrier, tissue engineering, wound dressing, biosenser and gene carrier. After it is compounded with other anions, the complexes can keep the good properties of chitosan, and avoid the biotoxicity produced by using chemical cross-linking agent. Chitosan-based polyelectrolyte can have strong electrostatic effect with grume, anionic surface and other giant molecules such as DNA, which can be effectively used in drug deliver, gene therapy and immobilized enzyme. Owing to the special properties of chitosan-based polyelectrolyte glue, such as pH value, ionic strength and electric field sensitivity, it can respond to the changes of external environment and realize the imitation of biological tissues as well as the stimulation of drug controlled release. Besides, chitosan has excellent bacterinertness, hemostatic, hydrophilicity and ventilation properties, which can promote wound healing effectively. Related Articles | Metrics

Select

Progress and prospect of scaffold materials for tissue-engineered heart valves Li Wen-tong, Yu Ben-tong 2012, 16 (43):  8133-8138.  doi: 10.3969/j.issn.2095-4344.2012.43.031 Abstract ( 353 )   PDF (686KB) ( 559 )   Save BACKGROUND: The mechanical and biological valves currently available for clinical use have crucial limitations. But tissue-engineered heart valves can avoid these problems and become an ideal substitute for biological valves. OBJECTIVE: To review the progress in the research of scaffold materials for tissue-engineered heart valves. METHODS: A computer-based online search of Wanfang and PubMed databases from 1990 to 2011 was conducted. The key words were “tissue engineering, heart valve, scaffold materials” in Chinese and English, respectively. A total of 147 articles were retrieved, and finally 61 articles were included in result analysis. RESULTS AND CONCLUSION: At present, heart valve replacement is the primary surgical treatment for heart valve diseases. Neither mechanical nor biologic heart valve is an ideal substitute, which has significant limitations in terms of durability, growth potential, compatibility, resistance to infection. However, the tissue-engineered heart valve that is a living organ with the capability for growth, repair and remodeling in the same way that the native heart valve does has the potential to be an ideal valve substitute. Related Articles | Metrics

Select

Application prospect of biological materials to prevent tendon adhesion induced by sports injuries Li Long-mei 2012, 16 (43):  8139-8143.  doi: 10.3969/j.issn.2095-4344.2012.43.032 Abstract ( 319 )   PDF (656KB) ( 492 )   Save BACKGROUND: High-tech biological materials can well prevent the adhesion between tendon and tendon sheath. OBJECTIVE: To evaluate the effects of different biological materials on preventing the adhesion between tendon and tendon sheath after the repair of sports injuries, and to search for suitable biological materials. METHODS: The keywords of “biological material, sports, tendon injury, antistick” were used in Chinese and English, respectively. A computer search of China Academic Journal Full-text database and PubMed database from January 1990 to March 2011 was performed. RESULTS AND CONCLUSION: The new-type biological materials such as chitosan, mechanisms of chitosan, chitosan/ poly lactic-co-glycolic acid film and sodium hyaluronate can prevent tendon adhesion induced by sports injuries effectively. However, all kinds of materials have their advantages and disadvantages, and therefore, the development direction in the future is to prevent tendon adhesion after sports injuries by reasonable use of various drugs or high-tech biological materials. Related Articles | Metrics

Select

Dental metallic materials and imaging artifacts: Detection and comparison Yu Hua, Zhang Xiao-dong, Wang Yi-jing, Wei Jing, Liu Jiao-jiao 2012, 16 (43):  8144-8151.  doi: 10.3969/j.issn.2095-4344.2012.43.033 Abstract ( 401 )   PDF (798KB) ( 633 )   Save BACKGROUND: Magnetic resonance imaging is commonly used in clinical imaging detection. The dental metallic materials may produce the imaging artifacts during magnetic resonance imaging detection which may influence the quality of the image and the accuracy of the diagnosis. OBJECTIVE: To analyze the intensity of the artifacts produced by different dental metallic materials in magnetic resonance imaging as well as the influential factors to the artifacts. METHODS: A total of nine articles related to the effect of different dental metallic materials on magnetic resonance imaging in the past 10 years were analyzed. The size of the artifacts produced by the commonly used dental metallic materials was analyzed from different aspects, such as the cobalt-chromium alloy, nickel-chromium alloy, titanium alloy, gold-platinum alloy, silver platinum alloys and pure titanium. The factors that affect the intensity of the artifacts were compared. RESULTS AND CONCLUSION: Cobalt-chromium alloy can produce the biggest artifact in the magnetic resonance imaging, and the artifact is increased with the enhancement of strength of the magnetic field. The artifact strength produced by the hard cobalt-chromium alloy is bigger than that produced by the soft cobalt-chromium alloy; the artifact strength produced by nickel-chromium alloy is bigger, and the smallest artifact strength is produced by gold-platinum alloy, silver platinum alloys and pure titanium. The interference factor of magnetic field intensity has no significant effect on the artifact size. Related Articles | Metrics

Select

Negative pressure wound therapy and biological semipermeable membrane covering for the treatment of diabetic skin chronic ulcers Zhong Mei, Yan Xiao-dong, Xu Guo-ling, Huang Xiu-lu, Tan Xiao-yan, Nong Yue-chou 2012, 16 (43):  8152-8158.  doi: 10.3969/j.issn.2095-4344.2012.43.034 Abstract ( 312 )   PDF (408KB) ( 613 )   Save BACKGROUND: Because of the malnourished condition and low immunity, the chronic diabetic skin ulcer is difficult to heal and the conventional wound treatment is ineffective. OBJECTIVE: To evaluate the efficacy and safety of negative pressure wound therapy on the treatment of diabetic skin chronic ulcers. METHODS: 144 patients with diabetic skin chronic ulcers (132 with diabetic foot ulcers and 12 with pressure sore) were collected from the Department of Endocrinology, the People’s Hospital of Guangxi Zhuang Autonomous Region during July 2007 to December 2010. The patients were treated with negative pressure wound therapy and routine treatment method respectively, and supplemented with blood sugar controlling and blood pressure controlling, anticoagulation, anti-infective and other symptomatic and supportive treatment. RESULTS AND CONCLUSION: Among the 109 patients that treated with negative pressure wound therapy, 60 patients were healed, 30 patients significant effective, 14 patients were effective, and 5 patients were ineffective with total effective rate of 95.4%. Among the 35 patients that treated with routine treatment method, 17 patients were healed, 10 patients were significant effective, three patients were effective, and five patients were ineffective with total effective rate of 85.7%. There was a significant difference in the total effective rate between two groups (P < 0.05). Negative pressure wound therapy could significantly improve the tissue oxygen partial pressure around the ulcers of the patients with diabetic foot ulcers, and the maximum continuous operation time of the negative pressure pump was 120 days without complications. It indicates that negative pressure wound therapy is safe and effective for the treatment of chronic diabetic skin ulcer. Related Articles | Metrics

Select

Clinical comparison of reimplanted metallic and plastic biliary stents for occluded primary metallic stents in malignant biliary obstruction Zhang Nuo-bei, Huang Nian-gen 2012, 16 (43):  8159-8162.  doi: 10.3969/j.issn.2095-4344.2012.43.035 Abstract ( 365 )   PDF (363KB) ( 520 )   Save BACKGROUND: Reimplantation of metallic and plastic biliary stents in the obstructed scents can solve obstruction. However, it is debatable what a kind of combinations in stents can have more benefits for patients. OBJECTIVE: To compare clinical effects of the reimplanted different kinds of stents for occluded primary metallic stents in malignant biliary obstruction. METHODS: Totally 83 patients with malignant biliary obstruction were collected. They underwent endoscopic metal biliary endoprothesis and secondary biliary stent placement in the medium and low levels of the other stent as “stent-in-stent” in the Second Affiliated Hospital of Nanchang University. The patients were divided into three groups according various combinations in primary and secondary implanted stents: covered metallic stent group, uncovered metallic stent group and plastic stent group (5 covered-plastic, 10 uncovered-plastic). RESULTS AND CONCLUSION: After the primary metallic stent was obstructed, the patent time of reimplanted covered metallic stents was significantly longer than that of plastic stents (P < 0.05). Compared with the uncovered metallic stent group, the cumulative patent time in the covered metallic stent group was obviously longer (P < 0.05-0.01). These results suggest that as the primary metallic stents were obstructed, covered metallic stents (at least one in the primary and/or secondary procedure) can provide longer cumulative patent time than uncovered metallic stents in both procedures for malignant biliary obstruction at medium and low levels treated by mendoscopic metal biliary endoprothesis. Related Articles | Metrics

Select

Diagnostic value of dual-source CT coronary angiography in detecting coronary-stent restenosis Hu Gang-feng, Bao Jian, Qian Ping-yan, Qian Bin, Hu Xiao-hua, Wu Jing-tao 2012, 16 (43):  8163-8167.  doi: 10.3969/j.issn.2095-4344.2012.43.036 Abstract ( 367 )   PDF (431KB) ( 401 )   Save BACKGROUND: The traditional CT is hard to be popularized in follow-up after the treatment of coronary heart disease with interventional stents, looking for noninvasive and effective method to evaluate the stent restenosis has became the hot spot. OBJECTIVE: To investigate the diagnosis value of dual-source CT coronary angiography in detecting coronary-stent restenosis through the comparing the evaluative effect of dual-source CT coronary angiography and selective coronary angiography without heart rate control. METHODS: The 53 cases with a total of 75 stents were performed with dual-source CT coronary angiography and coronary angiography to evaluate image quality and analyze sensitivity of coronary-stent restenosis. RESULTS AND CONCLUSION: ①All stents were considered assessable for diagnosis with dual-source CT angiography. Images quality of 96% (72/75) of the stents was good. ②There was no significant difference in sensitivity, specificity, positive and negative predictive value and the accuracy between dual-source CT angiography diagnose in-stent restenosis and coronary angiography diagnose in-stent restenosis (P > 0.05). ③There was no significant difference in sensitivity, specificity, positive and negative predictive value and the accuracy between the patients with the heart rate < 70 beats/min and ≥ 70 beats/min receiving dual-source CT angiography diagnosis for in-stent restenosis (P > 0.05). ④The dual-source CT angiography had lower sensitivity, specificity, positive and negative predictive value and accuracy to detect < 3.0 mm in-stent restenosis than to detect ≥ 3.0 mm in-stent restenosis (P < 0.05). The dual-source CT is helpful for the evaluation of coronary in-stent restenosis without heart rate control, and it can be used as the large-diameter stent patency assessment method and small-diameter stent preliminary screening method. Related Articles | Metrics

Select

Leukocyte reduction associated with Ni-Cr alloy porcelain crown: A case analysis Fan Can-can, An Xiao-li, Che Xiao-qiang, Liu Bin 2012, 16 (43):  8168-8170.  doi: 10.3969/j.issn.2095-4344.2012.43.037 Abstract ( 292 )   PDF (334KB) ( 454 )   Save BACKGROUND: It is known that Ni-Cr alloy porcelain crown can cause allergic reactions. Recently, their effects to whole body organs and functions also have attracted scholars' attention, especially for the influence on the kidney and immune system. OBJECTIVE: To report and analyze a clinical suspected case of lower white blood cell count due to the Ni-Cr alloy porcelain crown, and to discuss the impact of Ni-Cr alloy porcelain crown on the immune system. METHODS: According to the clinical special case, we inspected the general and oral conditions and removed the prosthesis which might cause diseases. Then we treated the related disease and restored the teeth with full porcelain crown. After a month, the patient came back for review and we compared the results of hemogram before and after treatment. Referring to literatures, we also analyzed the possibility of white blood cell count reduction caused by Ni-Cr alloy porcelain crown. RESULTS AND CONCLUSION: The change of white blood cell count after the removal of Ni-Cr alloy porcelain crown and all-ceramic crowns restoration could verify the conjecture that the Ni-Cr alloy porcelain crown caused the reduction of white blood cell count. The data also showed that the Ni-Cr alloy porcelain crown could influence the immune function through certain ways, which should arouse the attention of dental doctors. Therefore we should take certain measures to avoid these negative effects. Related Articles | Metrics