Abstract:

BACKGROUND: The antimicrobial activity of root canal filling materials in clinical application is limited at present. Root canal filling materials have detects, such as strong stimulation to tissues, difficulty in formation, bad root canal sealing and easy to be degradable.
OBJECTIVE: To study the effect of antimicrobial activity of nano-hydroxyapatite composite root canal filling materials with different pH values on common bacteria infected root canal in vitro.
METHODS: In vitro infected root canal model of enterococcus faecalis and candida albicans was established. The antibacterial property of nano-hydroxyapatite paste with different pH values in root canal in vitro was evaluated, including pH 8, pH 9 and pH 10. Zinc oxide eugenol were as a control.
RESULTS AND CONCLUSION: All the root canal filling materials have bacteriostatic effect. Compared with the control group, the inhibiting effect of nano-hydroxyapatite paste in each pH value group on enterococcus faecalis was better than that in the control group (P < 0.05). There was no significant difference among nano-hydroxyapatite paste in each pH value group (P > 0.05). The inhibitory effect of nano-hydroxyapatite paste in each pH value group on candida albicans was not better than that in the control group (P < 0.05). Nano-hydroxya paste in each pH value group was compared, and pH 10 group > pH 9 group > pH 8 group (P < 0.05). These findings suggest that in the model of infected root canal, the inhibiting effect of nano hydroxyapatite paste on enterococcus faecalis was better than that of zinc oxide eugenol (P < 0.05), but the inhibiting effect of nano-hydroxyapatite paste on candida albicans was not better than that of zinc oxide eugenol (P < 0.05). Besides, nano-hydroxyapatite composite root canal filling materials with pH=10 have the best bacteriostatic property.