Abstract:

BACKGROUND: Collagen and hyaluronic sacid are both helpful to cell adhesion, proliferation and differentiation in the tissue culture.
OBJECTIVE: To investigate the cytocompatibility between the three scaffolds (collagen, hyaluronic acid membrane, and latin sponge) and vascular endothelial cells, smooth muscle cells, as well as to screen the optimal seeding method.
METHODS: The passage 3-5 vascular smooth muscle cells of rabbits were seeded onto the collagen/hyaluronic acid membrane (or latin sponge); after cultured for 2 weeks, rabbit vascular endothelial cells were seeded onto the compound of smooth muscle cells-collagen/hyaluronic acid membrane (or latin sponge). Pure endothelial cells and smooth muscle cells were performed inoculation together.
RESULTS AND CONCLUSION: ①Light microscope and scanning electron microscope results showed that the inoculation times and growth were increased with culture time, and the cells on the collagen/hyaluronic acid membrane grew better, what’s more, cells attached more tightly. ②WST-1 method results showed that the adhesion rate and proliferation rate of smooth muscle cells in the collagen/hyaluronic acid membrane group were both higher than the latin sponge group (P < 0.05). Besides, cells growth on the scaffolds was increased in various degrees. ③³H-TDR method results indicated that DNA synthesis of cells in the collagen/hyaluronic acid membrane group was highest, and that in the latin sponge group was lower. These findings suggest that collagen/hyaluronic acid membrane has ideal cytocompatibility. Repeating seeding with proper interval can increase the adhesion and proliferation of cells on scaffolds.