Abstract:

BACKGROUND: The preliminary studies have found that there is an interaction effect of high sugar and ceramic alloy extracts on proliferation of L-929 mouse fibroblasts.
OBJECTIVE: To observe the effects of three kinds of noble ceramic alloys and high glucose on secrets of interleukin-6 from L-929 cells.
METHODS: Using two-factor factorial design experiments, we detected the changes of interleukin-6 levels in the extracellular fluid after co-culture of 89% Au-alloy, 74% Au-alloy and Ag-Pd alloy with mouse L-929 cells under different glucose concentrations (11.1, 22.2, 33.3 mmol/L). Cells without alloy served as negative controls.
RESULTS AND CONCLUSION: Two-factor factorial design results showed that the main effect of noble ceramic alloy extracts was statistically significant (P=0.001). The interleukin-6 levels of the supernatant in the 74% Au-alloy and the Ag-Pd alloy were significantly lower than that in the negative group (P=0.004, 0). The main effect of glucose concentration was also of statistical significance. The interleukin-6 level in supernatant in 33.3 mmol/L high glucose was significantly lower than that in 11.1 mmol/L glucose. However, no interaction existed between different extracts of noble ceramic alloys and glucose concentrations about the secrets of interleukin-6 from L-929 cells (P=0.33).