Chinese Journal of Tissue Engineering Research ›› 2012, Vol. 16 ›› Issue (43): 8055-8060.doi: 10.3969/j.issn.2095-4344.2012.43.017

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In vitro cellular biocompatibility of konjac glucomannan-hyaluronic acid blend membrane

Gu Shao, Pan Xian-feng, Xu Yong-qing, Chen Jia, Chen Qing-hua   

  1. Department of Orthopedics, Kunming General Hospital, Chengdu Military Area Command of Chinese PLA, Clinical Teaching Hospital of Kunming Medical University, Kunming 650032, Yunnan Province, China
  • Received:2012-01-11 Revised:2012-03-11 Online:2012-10-21 Published:2012-10-21
  • Contact: Pan Xian-feng, Doctor, Associate chief physician, Department of Orthopedics, Kunming General Hospital, Chengdu Military Area Command of Chinese PLA, Clinical Teaching Hospital of Kunming Medical University, Kunming 650032, Yunnan Province, China panxianfeng@ Yahoo.com.cn
  • About author:Gu shao★, Studying for master’s degree, Department of Orthopedics, Kunming General Hospital, Chengdu Military Area Command of Chinese PLA, Clinical Teaching Hospital of Kunming Medical University, Kunming 650032, Yunnan Province, China 18788144001@ 126.com

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Abstract:

BACKGROUND: Hyaluronic acid and konjac glucomannan can be used for the prevention of adhesion after surgery.
OBJECTIVE: To investigate the biocompatibility of konjac glucomannan-hyaluronic acid blend membrane co-cultured with bone marrow mesenchymal stem cells (BMSCs) of rabbits.
METHODS: The passage 3 BMSCs were cultured with konjac glucomannan-hyaluronic acid blend membrane in vitro. Their compound degree was observed by inverted microscope and scanning electron microscopy. Cell proliferation was detected by MTT assay. The BMSCs on the konjac glucomannan-hyaluronic acid blend membrane were orientiatedly induced into adipocytes in vitro. Differentiation effect was detected by oil red O staining
RESULTS AND CONCLUSION: BMSCs were well cultured with konjac glucomannan-hyaluronic acid blend membrane. Good adhesion and proliferation was found under inverted microscope and scanning electron microscope. The BMSCs on the konjac glucomannan-hyaluronic acid blend membrane could be orientiatedly induced into adipocytes, and had odipogenic potential. These findings suggest that there is a good biocompatibility between BMSCs and the konjac glucomannan-hyaluronic acid blend membrane.

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