Chinese Journal of Tissue Engineering Research ›› 2012, Vol. 16 ›› Issue (43): 8090-8093.doi: 10.3969/j.issn.2095-4344.2012.43.023

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Pathological changes of the corneal epithelial tissue in ophthalmoxerosis rabbits after treatment with chitosan/polyacrylic acid in situ gel

Zhang Jie, Wei Kun, Wei Xin-miao, Ling You, Xu Wei-kang   

  1. National Engineering Research Center for Tissue Restoration and Reconstruction, School of Material Science and Engineering, South China University of Technology, Guangzhou 510006, Guangdong Province, China
  • Received:2012-01-19 Revised:2012-03-10 Online:2012-10-21 Published:2012-10-21
  • Contact: Wei Kun, Professor, National Engineering Research Center for Tissue Restoration and Reconstruction, College of Material Science and Engineering, South China University of Technology, Guangzhou 510006, Guangdong Province, China weikun@scut.edu.cn
  • About author:Zhang Jie★, Studying for master’s degree, National Engineering Research Center for Tissue Restoration and Reconstruction, College of Material Science and Engineering, South China University of Technology, Guangzhou 510006, Guangdong Province, China 568005890@qq.com

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Abstract:

BACKGROUND: Chitosan (CS) has various good properties, such as reproducible, broad sources, easy degradation and excellent biocompatibility, which is a potential raw material for the preparation of in situ forming gel.
OBJECTIVE: Investigate the repairing effectiveness of CS/polyacrylic acid (PAA) in situ forming gel on the corneal tissue in rabbits with ocular injury.
METHODS: Twenty rabbits were collected to establish rabbit models of ophthalmoxerosis. Fourteen days after surgery, all rabbits were randomly divided into experiential group and control group. The rabbits in the experiential group were treated with CS/PAA in situ forming gel at ocular surface, three times per day. The rabbits in the control group had drug treatment.
RESULTS AND CONCLUSION: After treated with the CS/PAA in situ forming gel, the ultrastructure of the corneal epithelium of rabbits was close to normal, and epithelial cells were intact and closely arranged. The defect area was covered with the newborn epithelial cells with regular arrangement, and edema was rare. Meanwhile, the microvilli and micro wrinkles of epithelial cells were increased obviously, indicating the as-prepared preparation was effective for the treatment of ophthalmoxerosis. These findings suggest that the CS/PAA in situ forming gel can repair the corneal epithelial tissue in ophthalmoxerosis.

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