Chinese Journal of Tissue Engineering Research ›› 2012, Vol. 16 ›› Issue (43): 8094-8099.doi: 10.3969/j.issn.2095-4344.2012.43.024

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Application of Micro-CT combined with epidurography in medical self-crosslinked sodium hyaluronate gel for the prevention of epidural adhesions after laminectomy

Liang Wen-kai1, Guo Quan-yi2, Han Shu-feng1, Zhang Li2, Peng Jiang2, Wang Ai-yuan2, Sui Xiang2, Liang Zeng-yi3, Xu Wen-jing2, Lu Shi-bi2   

  1. 1Department of Orthopedics, First Clinical Medical College, Shanxi Medical University, Taiyuan 030001, Shanxi Province, China
    2Institute of Orthopedics, General Hospital of Chinese PLA, Beijing 100853, China
    3Beijing ShenYou Biological Technology Co., Ltd., Beijing 100853, China
  • Received:2012-02-08 Revised:2012-03-18 Online:2012-10-21 Published:2012-10-21
  • Contact: Guo Quan-yi, Chief physician, Institute of Orthopedics, General Hospital of Chinese PLA, Beijing 100853, China doctorguo@163.com
  • About author:Liang Wen-kai★, Studying for master’s degree, Department of Orthopedics, First Clinical Medical College, Shanxi Medical University, Taiyuan 030001, Shanxi Province, China ivwvi@163.com

Abstract:

BACKGROUND: Medical self-crosslinked sodium hyaluronate gel can effectively prevent epidural adhesions after laminectomy in rabbits within 8 weeks after operation, which has been confirmed by planar angle.
OBJECTIVE: To observe and evaluate the practicability of self-crosslinked sodium hyaluronate gel in preventing epidural adhesions by using Micro-CT combined with epidurography.
METHODS: Eighteen new Zealand white rabbits with total lumbar laminectomy at L5 level were randomly divided into three groups: control, HyaRegen/SPⅠ and HyaRegen/SPⅡ groups. In the control group, only normal saline was used to flush the laminectomy area before wound closure, while in HyaRegen/SPI and HyaRegen/SPⅡ groups, the exposed dura mater was covered with 0.5 mL HyaRegen/SPI or 0.5 mL HyaRegen/SPII, respectively before wound closure.
RESULTS AND CONCLUSION: The latency of cortical somatosensory evoked potentials was not delayed obviously in each group, and there was no significant difference in the three groups (P > 0.05). The scanning of Micro-CT combined with epidurography and the images of three-dimensional reconstruction showed that contrast agent could fill the epidural space smoothly in the HyaRegen/SPⅠ and HyaRegen/SPⅡ groups, but not in the control group, and many filling defects in local operation area could been observed. In addition, the filling volume of contrast agent of the HyaRegen/SPⅠ and HyaRegen/SPⅡ groups in the unit volume of operation area was higher than that in the control group (P < 0.05). There was no significant difference between HyaRegen/SPI and HyaRegen/SPⅡ groups (P > 0.05). Moreover, the degree of epidural adhesions of the control group was significantly higher than that in the HyaRegen/SPI and HyaRegen/SPⅡ groups (P < 0.05). These findings suggest that medical self-crosslinked sodium hyaluronate gel can effectively prevent epidural adhesions after laminectomy in rabbits. Besides, epidural adhesions can be observed and evaluated effectively by using Micro-CT combined with epidurography.

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