Physicochemical properties of chitosan microspheres/silk fibroin/calcium sulfate bone cement

doi:10.3969/j.issn.2095-4344.2014.12.005

Abstract

Abstract:

BACKGROUND: Calcium sulfate used in kyphoplasty and vertebrolplasty has good physical and chemical properties, exerts no toxic effects on human body and has the degradation performance. But its main drawback is rapid degradation.
OBJECTIVE: To develop a chitosan microsphere with silk fibroin/calcium sulfate cement to prepare drug carrier system.
METHODS: Chitosan microspheres were prepared by the emulsion method. Scanning electron microscopy, particle size analysis and swelling rate were used to study the properties of the microspheres. Different silk concentrations (3%, 6% and 9%) and weight rates (0.5%,1% and 5%) of chitosan microspheres were used to determine the best formula which has the strongest mechanical properties. The composition of this composite bone cement was detected by using X-ray diffraction and Fourier transform infrared spectroscopy.
RESULTS AND CONCLUSION: When the concentration of silk fibroin was 6% and weight rate of chitosan microspheres was 0.5%, we could obtain the maximum compressive strength, which was (39.17±1.96) MPa. With this composition, the initial setting time was (12.99±1.63) minutes and the final setting time was (21.55±0.54) minutes. The results from X-ray diffraction and Fourier transform infrared spectroscopy demonstrated that the main phase composition was calcium sulfate, and silk and chitosan were also included. The composite chitosan microspheres exhibited a slightly wrinkled surface, but were still intact in spherical shape, indicating the preparation of chitosan microspheres/silk fibroin/calcium sulfate cement was reliable and the product had good structures and properties.

中国组织工程研究杂志出版内容重点：生物材料；骨生物材料; 口腔生物材料; 纳米材料; 缓释材料; 材料相容性；组织工程

全文链接：

Key words: biocompatible materials, calcium sulfate, chitosan, silk

CLC Number:

R318

Wang Peng, Pi Bin, Wang Jin-ning, Zhu Xue-song, Yang Hui-lin . Physicochemical properties of chitosan microspheres/silk fibroin/calcium sulfate bone cement[J]. Chinese Journal of Tissue Engineering Research, 2014, 18(12): 1831-1838.

Figures/Tables 4

2.3.3 扫描电镜观察单纯硫酸钙骨水泥(图4A)显示骨水泥中形成细针样颗粒，颗粒间孔隙较大。丝素蛋白/硫酸钙骨水泥(图4B)显示硫酸钙成短棒状或颗粒状，晶体间有均匀物质将颗粒相互连接，颗粒间孔隙减小，表面更加致密。丝素浓度为6%、微球含量为0.5%的复合骨水泥(图4C)显示骨水泥中晶体成细针状，相互搭接，排列紧密，晶体间可见团块状物质存在。图4D为丝素浓度为6%、壳聚糖微球含量为0.5%的复合骨水泥中微球的微观形态，可以看出微球表面稍有皱缩，但球形仍然完整，未见明显破坏，可见在制备复合骨水泥的过程中微球能保持稳定而不被破坏。 2.4 不同骨水泥的力学性能比较 1-7组骨水泥的抗压强度分别为(22.49±2.88)，(22.53±1.83)，(31.09±0.74)，(27.49±2.99)，(39.17±1.96)，(27.07±2.47)，(4.72± 0.52) MPa。含有不同丝素浓度骨水泥的抗压强度差异有显著性意义(P < 0.05)，不同组两两比较显示，1、2组间比较差异无显著性意义(P > 0.05)，其余各组间抗压强度差异有显著性意义(P < 0.05)。图5A显示了不同丝素浓度骨水泥抗压强度的变化趋势，并可知加入丝素后材料的抗压强度明显提高，且丝素浓度为6%时复合骨水泥的抗压强度达到最大。含有不同比例壳聚糖微球的骨水泥抗压强度差异有显著性意义(P < 0.05),不同组两两比较显示，各组间抗压强度差异有显著性意义(P < 0.05)。图5B显示含有不同质量比壳聚糖微球骨水泥抗压强度的变化趋势，当壳聚糖含量为0.5%时复合骨水泥的抗压强度达到最大，随着壳聚糖微球的加入，复合骨水泥的强度显著下降。 2.5 不同骨水泥的抗稀散性 3种骨水泥投入PBS后 10 min，均未见明显溃散(图6A)，24 h后发现单纯硫酸钙骨水泥溃散明显，而另外两种骨水泥仍成球形(图6B)，48 h后可见单纯硫酸钙骨水泥继续溃散，壳聚糖微球/丝素蛋白/硫酸钙骨水泥周围也出现了少许碎片，而丝素蛋白/硫酸钙骨水泥仍然完好无损(图6C)，实验说明硫酸钙中加入丝素蛋白可以增强骨水泥的抗稀散性，而微球的加入则会减弱骨水泥的抗稀散性。"

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