Effects of calcined bone/chitosan composite materials on proliferation and adhesion in osteoblasts

doi:10.3969/j.issn.2095-4344.2357

Abstract

Abstract:

BACKGROUND: The different ratios of calcined bone/chitosan composites were prepared in previous studies, and in vitro cell experiments showed that the composites were safe and non-toxic.

OBJECTIVE: To observe the effect of calcined bone/chitosan composite on the proliferation and adhesion of osteoblasts.

METHODS: The composite materials with calcined bone and chitosan mass ratios of 1/2, 1/1, and 2/1 were prepared by solution blending method. Neonatal rat osteoblasts were inoculated on three kinds of composite materials, and the cells cultured alone were used as control. CCK-8 kit was used to detect cell proliferation. DAPI fluorescence staining was used to observe the growth of cells on the material. Scanning electron microscopy was used to observe the adhesion of cells on the surface of the composite material.

RESULTS AND CONCLUSION: (1) The CCK-8 test showed that the osteoblasts of the four components proliferated well, proliferated slowly from the first to third days of culture, accelerated after the third day, entered the logarithmic growth phase from the third to seventh days, and entered the plateau phase after the seventh day. There was no difference in cell proliferation between the composite materials group and the control group. (2) After 3 days of culture, DAPI fluorescent staining showed that osteoblasts grew well on the surface of the three kinds of composites, and there was no significant difference between the groups. (3) Scanning electron microscopy showed that after 3 days of culture, the osteoblasts adhered tightly to the surface of the three kinds of composites, stretched completely; the cell body was full and protruded the pseudopod that was embedded in the material. After 7 days of culture, the cells covered the surface of the material and grew densely, among which the cells on the surface of calcined bone/chitosan composite with a mass ratio of 2/1 were most dense. (4) The results showed that osteoblasts could proliferate and adhere on the surface of the calcined bone/chitosan composite material.

Key words: bone">, , material, chitosan">, , calcined bone">, , osteoblast">, , cell proliferation">, , cell adhesion">, , bone regeneration

CLC Number:

Liao Jian, Huang Xiaolin, Huo Hua, Zhou Qian, Cheng Yuting, Qi Yuhan, Wu Chao, Yang Tongjing, Liao Yunmao, Liang Xing. Effects of calcined bone/chitosan composite materials on proliferation and adhesion in osteoblasts[J]. Chinese Journal of Tissue Engineering Research, 2020, 24(34): 5447-5453.

Figures/Tables 8

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