Preparation and properties of hydrophilic modified artificial bone scaffold using low-temperature rapid prototyping

doi:10.3969/j.issn.2095-4344.2014.12.004

Abstract

Abstract:

BACKGROUND: Low-temperature rapid prototyping technology is a new kind of rapid prototyping technology, and it is rapidly used in the preparation of bone tissue engineering scaffolds because it can make scaffold forming controllable and can keep the biological activity of the materials, also can easily realize the scaffold with porous of three-dimensional structure and other advantages.
OBJECTIVE: To investigate the preparation process of polyethylene glycol-modified polylactic acid-glycolic acid/nano-hydroxyapatite (PLGA-PEG/n-HA) using the low-temperature rapid prototyping, and to test its performance.
METHODS: PLGA-PEG/n-HA and PLGA/n-HA were prepared by low-temperature rapid prototyping equipment. Under an electron microscopy, we observed ultra-structure of the scaffolds. Immersion (ethanol) method was used to test the porosity, and electronic testing machine was used to determine the material mechanical properties. Then these two kinds of scaffolds with rat osteoblasts were cultured in vitro, the cell adhesion rate was detected by precipitation method after 12 hours, and cell counting kit-8 method was used to determine the cell proliferation at culture days 1, 3, 5, 7, 9, 12.
RESULTS AND CONCLUSION: Both of the two scaffolds had ideal aperture range and high porosity. But the aperture range of PLGA-PEG/n-HA scaffolds had large fluctuations, and the average aperture was smaller than that of PLGA/n-HA. Some pores were closed up. The cell adhesion rate and the cell growth curve of PLGA-PEG/n-HA was better than that of PLGA/n-HA (P < 0.05), but the mechanical properties were less than PLGA/n-HA (P < 0.05). The results showed the PLGA-PEG/n-HA scaffolds had good cell compatibility.

中国组织工程研究杂志出版内容重点：生物材料；骨生物材料; 口腔生物材料; 纳米材料; 缓释材料; 材料相容性；组织工程

全文链接：

Key words: tissue engineering, hypothermia, polyethylene glycols, durapatite

CLC Number:

R318

He Mei-jian, Wang Da-ping, Huang Jiang-hong, Zhang Ming . Preparation and properties of hydrophilic modified artificial bone scaffold using low-temperature rapid prototyping[J]. Chinese Journal of Tissue Engineering Research, 2014, 18(12): 1824-1830.

Figures/Tables 2

References

[1] 王呈,曾炳芳.骨移植替代材料在创伤骨科的应用[J].国际骨科学杂志, 2012,33(1):37-38.

[2] Shive MS,Anderson JM.Biodegradation and biocompatibility of PLA and PLGA microspheres.Adv Drug Deliv Rev.1997; 28(1):5-24.

[3] Park JS,Kang SK.A study on surface, Thermal and Mechanical Properties of Absorbable PLGA Plate.Int J Control Autom.2013;6(6):73-82.

[4] Makadia HK,Siegel SJ.Poly Lactic-co-Glycolic Acid(PLGA) as Biodegradable Controlled Drug Delivery Carrier.Polymers. 2011;3:1377-1397.

[5] Takayama T,Todo M,Takano A.The effect of bimodal distribution on the mechanical properties of hydroxyapatite particle filled poly(L-lactide) composites. J Mech Behav Biomed Mater.2009;2(1):105-112.

[6] Kuboki Y,Jin Q,Takita H.Geometry of carriers controlling phenotypic expression in BMP‐induced osteogenesis and chondrogenesis.J Bone Joint Surg Am.2001;83-A Suppl 1 (Pt 2):S105-115.

[7] Gajendiran M,Divakar S,Raaman N,et al.In vitro drug release behavior, mechanism and antimicrobial activity of rifampicin loaded low molecular weight PLGA-PEG-PLGA triblock copolymeric nanospheres.Curr Drug Deliv.2013;10(6):722-731.

[8] 李宁,刘斌,戎利民,等.静电纺丝聚乳酸聚乙醇酸/聚乙二醇纳米纤维作为组织工程支架的体外细胞相容性研究[J].中华创伤骨科杂志,2012,14(3):241-245.

[9] 张建明,张西正,李瑞欣,等.快速成型方法制备组织工程支架的研究和应用[J].中国组织工程研究,2013,17(8):1435-1440.

[10] Liu YY,Fang SH,Han ZZ,et al.Study on low-temperature deposition manufacturing process parameters of three-dimensional Chitosan scaffold. Key Eng Mater. 2012; 522:97-102.

[11] Liang YC,Zheng XF,Zhai WJ,et al.3D PLLA/nano-hydroxyapatite scaffolds with hierarchical porous structure fabricated by low-temperature deposition manufacturing.J Wuhan Univ Technol(Mater Sci Ed).2012; 27(2):265-269.

[12] 李晓峰,赵劲民,苏伟,等.大鼠成骨细胞的原代培养和鉴定[J].中国组织工程研究与临床康复,2011,15(6):990-994.

[13] 王勇平,廖燚,蒋垚.成骨细胞的体外培养与鉴定[J].中国组织工程研究与临床康复,2011,15(33):6231-6234.

[14] Zhao T,Ling ZQ,Yu WQ,et al.Adhesive properties of hepatoma cell to collagen IV coated surfaces.Hepatobiliary Pancreat Dis Int.2002;1(4):565-569.

[15] Jiang LY,Xiong CD,Jiang LX,et al.Effect of HA with different grain size range on the crystallization behaviors and mechanical property of HA/PLGA composite. 2013;565:52-57.

[16] Jang JH,Castano O,Kim HW.Electrospun materials as potential platforms for bone tissue engineering.Adv Drug Deliv Rev.2009;61(12):1065-1083.

[17] 余东,雷华,唐晓军,等.低温快速成形人工骨的敷贴植骨实验研究[J].中国美容医学,2008,17(1):75-79.

[18] Xu M,Li Y,Suo H,et al.Fabricating a pearl/PLGA composite scaffold by the low-temperature deposition manufacturing technique for bone tissue engineering. Biofabrication. 2010; 2(2):025002.

[19] Liu L,Xiong Z,Yan Y,et al.Porous morphology, porosity, mechanical properties of poly(alpha-hydroxy acid)-tricalcium phosphatecomposite scaffolds fabricated by low-temperature deposition.J Biomed Mater Res A.2007;82(3):618-629.

[20] Mehdizadeh H,Sumo S,Bayrak ES,et al.Three-dimensional modeling of angiogenesis in porous biomaterial scaffolds. Biomaterials.2013;34(12):2875-2887.

[21] Kuboki Y,Jin Q,Kikuchi M,et al.Geometry of artificial ECM: sizes of pores controlling phenotype expression in BMP-induced osteogenesis and chondrogenesis. Connect Tissue Res.2002;43(2-3):529-534.

[22] Karageorgiou V,Kaplan D.Porosity of 3D biomaterial scaffolds and osteogenesis. Biomaterials.2005;26(27):5474-5491.

[23] Carletti E,Motta A,Migliaresi C.Scaffolds for tissue engineering and 3D cell culture. Methods Mol Biol.2011;695: 17-39.

[24] Kai H,Wang X,Madhukar KS,et al.Fabrication of a two-level tumor bone repair biomaterial based on a rapid prototyping technique. Biofabrication.2009;1(2):025003.

[25] Panetta NJ,Gupta DM,Longaker MT.Bone regeneration and repair. Curr Stem cell Ther.2010;5(2):122-128.

[26] Liu YY,Fang SH,Han ZZ,et al.Pneumatic feeding system for low-temperature deposition manufacturing based on systemidentification.Virt Phys Protot.2013;8(1):3-9.

[27] Boyan BD,Hummert TW,Dean DD,et al.Role of material surfaces in regulating bone and cartilage cell response. Biomaterials.1996;17(2):137-146.

[28] Mallarde D,Boutignon F,Moine F,et al.PLGA-PEG microspheres of teverelix: influence of Polymer type on microspheres and on teverelix in vitro release.Int J Pharm. 2003;261(1-2):69-80.

[29] Stanford CM,Solursh M,Keller JC.Significant role of adhesion properties of primary osteoblast-like cells in early adhesion events for chondroitin sulfate and dermatan sulfate surface molecules.J Biomed Mater Res.1999;47(3):345-352.

Cheung HY,Lau KT,Lu TP,et al.A critical review on polymer-based bio-engineered materials for scaffold development.Comp Pt B: Engg.2007;38:291-300