Vascularization of vascular endothelial growth factor and collagen I modified beta-tricalcium phosphate porous scaffolds

doi:10.3969/j.issn.2095-4344.2014.12.006

Abstract

Abstract:

BACKGROUND: The auditory ossicle chain reconstruction is still an important method to treat conductive deafness. Although a great variety of materials have been applied, the blood supply of otosteon after the implantation is ignored. Moreover, there is no real bone formed.
OBJECTIVE: To observe the angiogenesis of vascular endothelial growth factor and collagen I modified β-tricalcium phosphate porous scaffold which is implanted into the otocyst of guinea pig.
METHODS: Totally 60 guinea pigs were randomly divided into experimental group (vascular endothelial growth factor and collagen I modified β-tricalcium phosphate porous scaffold), collagen I control group (collagen I modified β-tricalcium phosphate porous scaffold) and blank control group (β-tricalcium phosphate porous scaffold). The guinea pigs were executed under anesthesia at weeks 1, 2, 3, 4 respectively. The surface of scaffolds was observed by scanning electron microscopy. The angiogenesis of scaffolds were observed by hematoxylin-eosin staining and CD34 immunohistochemistry staining, and then the microvascular density was counted. The osteogenesis of the scaffolds was observed by toluidine blue staining.
RESULTS AND CONCLUSION: Endothelial cell proliferation and lumen formation could be observed after 1 week in the experimental group, and the angiogenesis reach the peak after 3 weeks with traffic branches formedbetween micropores. In the other two groups, the lumen formed at 2 weeks but no traffic branches were visible. The sprouting of new blood vessels in the pores were observed more in the experimental group than the other two groups (P < 0.05). The adherence and proliferation of cells could be examined in the surface and pores of the scaffold by scanning electron microscope. After 4 weeks, the osteogenesis could be observed by toluidine blue staining, especially in the experimental group. These findings suggest that the vascular endothelial growth factor and collagen I modified β-tricalcium phosphate porous scaffold can realize an effective vascularization in the environment of guinea pigs’ middle ear. What’s more, the scaffold also can promote bone formation.

中国组织工程研究杂志出版内容重点：生物材料；骨生物材料; 口腔生物材料; 纳米材料; 缓释材料; 材料相容性；组织工程

全文链接：

Key words: tissue scaffolds, ceramics, calcium phosphates, vascular endothelial growth factors, collagen type I, neovascularization, physiologic

CLC Number:

R318

Lu Meng-yi, Ren Yi, Hu Wan-qing, Gui Yuan, Zhang Long-cheng . Vascularization of vascular endothelial growth factor and collagen I modified beta-tricalcium phosphate porous scaffolds[J]. Chinese Journal of Tissue Engineering Research, 2014, 18(12): 1839-1845.

Figures/Tables 5

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