Vacuum sealing drainage enhances wound healing by up-regulating collagen type I/III ratio in rats

doi:10.3969/j.issn.2095-4344.2859

Abstract

Abstract:

BACKGROUND: Vacuum sealing drainage can enhance acute and chronic wound healing. The ratio of collagen type I/III play a critical role in the structural stability of skin tissue and skin repair, but its change during vacuum sealing drainage accelerating wound healing remains unclear.

OBJECTIVE: To observe the effect of vacuum sealing drainage on the ratio of collagen type I/III during wound healing and to explore the potential mechanism underlying acute wound repair in rats.

METHODS: A full-thickness wound, with a diameter of 20 mm, was created on the back of healthy male rats. All model rats were then randomized into two groups: blank control and vacuum sealing drainage groups. The wound surface was photographed at three observational time points (1, 3, 7 days after operation), and wound closure rate was calculated and compared. The mRNA and protein expression levels of type I collagen and type III collagen and ratio of collagen type I/III were detected by RT-qPCR and immunohistochemistry. The structure of granulation tissue and length of re-epithelialization were histologically detected.

RESULTS AND CONCLUSION: Compared with the blank control group, treatment with vacuum sealing drainage significantly increased the expression of type I collagen and type III collagen at mRNA and protein levels (P < 0.05), enhanced wound healing rate (P < 0.05) as well as increasing the ratio of collagen type I/III starting from the 3^rd day after operation (P < 0.05). To conclude, the vacuum sealing drainage can accelerate wound healing by up-regulating the protein expression of type I collagen and type III collagen, the ratio of collagen type I/III and increasing wound tensile strength.

Key words: vacuum sealing drainage, acute wound, collagen type I, collagen type III, the ratio of collagen types I/III, re-epithelialization, wound healing, granulation tissue, wound healing rate

CLC Number:

Zhao Xin, Shi Xin, Chen Bei, Cao Yanpeng, Chen Yaowu, Liu Xiaoren, He Yusheng, He Liyun, Li Xiying, Liu Jun. Vacuum sealing drainage enhances wound healing by up-regulating collagen type I/III ratio in rats[J]. Chinese Journal of Tissue Engineering Research, 2020, 24(32): 5122-5127.

Figures/Tables 5

References

[1] BARBIERI A, SABATINI L, GRAZIOSI F, et al. Occupational safety and health risks in dock work: a narrative literature review. Med Lav. 2014; 105(6):413-434.

[2] LIU T, BAI X. Trauma care system in China. Chin J Traumatol. 2018; 21(2):80-83.

[3] RUTTER L. Identifying and managing wound infection in the community. Br J Community Nurs. 2018;23(Sup3):S6-S14.

[4] AZIZ ARA, ALSABEK MB. Diabetic foot and disaster; risk factors for amputation during the Syrian crisis. J Diabetes Complications. 2020; 34(2):107493.

[5] GAO M, NGUYEN TT, SUCKOW MA, et al. Acceleration of diabetic wound healing using a novel protease-anti-protease combination therapy. Proc Natl Acad Sci USA. 2015;112(49):15226.

[6] KONG F, FAN C, YANG Y, et al. 5-hydroxymethylfurfural-embedded poly (vinyl alcohol)/sodium alginate hybrid hydrogels accelerate wound healing. Int J Biol Macromol. 2019;138:933-949.

[7] RAJAN V, MURRAY RZ. The duplicitous nature of inflammation in wound repair. Wound Pract Res. 2008;16(3):122-129.

[8] FLEISCHMANN W, STRECKER W, BOMBELLI M, et al. Vacuum sealing as treatment of soft tissue damage in open fractures. Unfallchirurg. 1993;96(9):488-492.

[9] FREAR C C, GRIFFIN B, CUTTLE L, et al. Study of negative pressure wound therapy as an adjunct treatment for acute burns in children (SONATA in C): protocol for a randomised controlled trial. Trials. 2019; 20(1):130.

[10] HYLDIG N, VINTER CA, KRUSE M, et al. Prophylactic incisional negative pressure wound therapy reduces the risk of surgical site infection after caesarean section in obese women: a pragmatic randomised clinical trial. BJOG. 2019,126(5):628-635.

[11] MILAN PB, LOTFIBAKHSHAIESH N, JOGHATAIE MT, et al. Accelerated wound healing in a diabetic rat model using decellularized dermal matrix and human umbilical cord perivascular cells. Acta Biomater. 2016;45:234-246.

[12] 张媛,解怡洁.不同负压值在负压伤口治疗中的作用[J].医学研究生学报, 2012,25(6):661-663.

[13] MA Z, LI Z, SHOU K, et al. Negative pressure wound therapy: regulating blood flow perfusion and microvessel maturation through microvascular pericytes. Int J Mol Med. 2017;40(5):1415-1425.

[14] RIEKKI R, PARIKKA M, JUKKOLA A, et al. Increased expression of collagen types I and III in human skin as a consequence of radiotherapy. Arch Dermatol Res. 2002;294(4):178-184.

[15] LIU X, WU H, BYRNE M, et al. Type III collagen is crucial for collagen I fibrillogenesis and for normal cardiovascular development. Proc Natl Acad Sci USA. 1997;94(5):1852.

[16] HIRE JM, EVANSON JL, JOHNSON PC, et al. Variance of matrix metalloproteinase (MMP) and tissue inhibitor of metalloproteinase (TIMP) concentrations in activated, concentrated platelets from healthy male donors. J Orthop Surg Res. 2014;9:29.

[17] RAHAJENG R. The increased of MMP-9 and MMP-2 with the decreased of TIMP-1 on the uterosacral ligament after childbirth. Pan Afr Med J. 2018;30:283.

[18] BROUGHTON G, JANIS JE, ATTINGER CE. Wound healing: an overview. Plast Reconstr Surg. 2006;117(7 Suppl):1e-S-32e-S.

[19] BUSNARDO VL, BIONDO-SIMÕES MLP. Effects of low-level helium-neon laser on induced wound healing in rats. Rev Bras fisioter. 2010;14(1):45-51.

[20] BIRK D E, MAYNE R. Localization of collagen types I, III and V during tendon development. Changes in collagen types I and III are correlated with changes in fibril diameter. Eur J cell Biol. 1997;72(4): 352-361.

[21] JACOBS S, SIMHAEE DA, MARSANO A, et al. Efficacy and mechanisms of vacuum-assisted closure (VAC) therapy in promoting wound healing: a rodent model. J Plast Reconstr Aesthet Surg. 2009; 62(10):1331-1338.

[22] 王翔,杨帆,解杰,等.负压封闭引流技术干预局部氧分压的实验研究[J].中华急诊医学杂志,2018,27(11):1218-1223.

[23] ELTZSCHIG HK, ECKLE T. Ischemia and reperfusion-from mechanism to translation. Nat Med. 2011;17(11):1391-1401.

[24] BOREA PA, GESSI S, MERIGHI S, et al. Adenosine as a multi-signalling guardian angel in human diseases: when, where and how does it exert its protective effects? Trends Pharmacol Sci. 2016; 37(6):419-434.

[25] SHAIKH G, ZHANG J, PEREZ-ASO M, et al. Adenosine A(2A) receptor promotes collagen type III synthesis via β-catenin activation in human dermal fibroblasts. Br J Pharmacol. 2016;173(23): 3279-3291.

[26] 杨少玲,朱旅云,孙蕾蕾,等.创面负压治疗上调糖尿病足创面肉芽组织TGFβ1表达[J].基础医学与临床,2017,37(9):1323-1325.

[27] LU F, OGAWA R, NGUYEN DT, et al. Microdeformation of three-dimensional cultured fibroblasts induces gene expression and morphological changes. Ann Plast Surg. 2011;66(3):296-300.

[28] 胡承浩,李东宇,庞宗超,等.封闭式负压引流技术治疗糖尿病足对创面组织中TGF-β1及其受体表达的影响研究[J].中国修复重建外科杂志,2018, 32(8):1061-1065.

[29] SUTO M, MASUTOMI H, ISHIHARA K, et al. A Potato peel extract stimulates type I collagen synthesis via Akt and ERK signaling in normal human dermal fibroblasts. Biol Pharm Bull. 2019;42(9): 1510-1516.

[30] VERRECCHIA F, MAUVIEL A. TGF-β and TNF-α: antagonistic cytokines controlling type I collagen gene expression. Cell Signal. 2004;16(8):873-880.

[31] LIU X, LONG X, LIU W, et al. Type I collagen induces mesenchymal cell differentiation into myofibroblasts through YAP-induced TGF-β1 activation. Biochimie. 2018;150:110-130.

[32] MÜLLER S A, DÜRSELEN L, HEISTERBACH P, et al. Effect of a simple collagen type i sponge for achilles tendon repair in a rat model. Am J Sports Med. 2016;44(8):1998-2004.

[33] ZHAO F, CHEN F, YUAN X, et al. Decreased collagen type III synthesis in skin fibroblasts is associated with parastomal hernia following colostomy. Int J Mol Med. 2019,44(5):1609-1618.

[34] XIE S, ZHOU Y, TANG Y, et al. -Book-shaped decellularized tendon matrix scaffold combined with bone marrow mesenchymal stem cells-sheets for repair of achilles tendon defect in rabbit. J Orthop Res. 2019;37(4):887-897.

[35] SEIDEL D, LEFERING R, NEUGEBAUER EAM. Treatment of subcutaneous abdominal wound healing impairment after surgery without fascial dehiscence by vacuum assisted closure™ (SAWHI-V.A.C.®-study) versus standard conventional wound therapy: study protocol for a randomized controlled trial. Trials. 2013;14:394.

[36] GOVINDARAJU P, TODD L, SHETYE S, et al. CD44-dependent inflammation, fibrogenesis, and collagenolysis regulates extracellular matrix remodeling and tensile strength during cutaneous wound healing. Matrix Biol. 2019;75-76:314-330.

[37] SQUIER CA. The stretching of mouse skin in vivo: effect on epidermal proliferation and thickness. J Invest Dermatol. 1980;74(2):68-71.