Biocompatibility of rat osteoblasts with chitosan/hydroxyapatite composite scaffold degradation products

doi:10.3969/j.issn.2095-4344.2014.12.007

Abstract

Abstract:

BACKGROUND: The in vivo degradation process of chitosan/hydroxyapatite composite porous scaffolds is not very clear. Research on the effects of rat osteoblasts and degradation products is less.
OBJECTIVE: To analyze the biocompatiblity of rat osteoblasts with degradation products of chitosan/hydroxyapatite composite porous scaffolds.
METHODS: The second generation of cultured rat osteoblasts were respectively cultured in the extract of degradation products of chitosan/hydroxyapatite composite scaffolds (experimental group) and Dulbecco’s modified Eagle’s medium containing 10% fetal bovine serum (control group). At 2, 4, 6, 8, 10 days of culture, cell counting was measured by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay. Alkaline phosphatase activity was measured by the recommended method of determination of the Federation, and total protein was determined by BCA method.
RESULTS AND CONCLUSION: The proliferation speed, alkaline phosphatase activity, total cellular protein synthesis and ratio of alkaline phosphatase to total protein in rat osteoblasts cultured in the experimental group were significantly higher than those in the control group (P < 0.05). This experiment showed that the degradation products of chitosan/hydroxyapatite composite porous scaffolds cannot only promote rat osteoblast adhesion, growth and proliferation, but also enhance its ossification function, with good biocompatibility.

中国组织工程研究杂志出版内容重点：生物材料；骨生物材料; 口腔生物材料; 纳米材料; 缓释材料; 材料相容性；组织工程

全文链接：

Key words: biocompatible materials, durapatite, chitosan, osteoblasts

CLC Number:

R318

Ning Si-min, Wang Dong, Sun Hai-yu, Li Shu-wei, Xu Kun, Yang Fan. Biocompatibility of rat osteoblasts with chitosan/hydroxyapatite composite scaffold degradation products[J]. Chinese Journal of Tissue Engineering Research, 2014, 18(12): 1846-1851.

Figures/Tables 3

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