Effects of Ginkgo biloba Pingchan Recipe on loss and apoptosis of dopamine neurons in mouse models of Parkinson’s disease

doi:10.3969/j.issn.2095-4344.2016.49.005

Abstract

Abstract:

BACKGROUND: Current pharmacological research has administrated that Ginkgo biloba and its extracts can eliminate loss and apoptosis of dopamine neurons by antioxidation and nerve growth factor activation.
OBJECTIVE: To confirm the effects of Ginkgo biloba Pingchan Recipe on loss and apoptosis of dopamine neurons as well as proliferation of neuroblastoma derived cell lines SH-SY5Y in mice with Parkinson’s disease.
METHODS: (1) C57BL mice were randomly divided into three groups: 20 mice were modeled into Parkinson’s disease by intraperitoneal injection of MPTP for 6 weeks, and at 30 minutes before each intraperitoneal injection, mice in model group were given gavage of normal saline, mice in treatment group given Ginkgo biloba Pingchan Recipe. The other 10 mice received no any interventions as normal group. The loss and apoptosis of dopamine neurons were observed by immunohistochemistry at 15 and 30 days after modeling. (2) In vitro cultured SH-SY5Y cell lines were treated with Ginkgo biloba Pingchan Recipe. Subsequently, the cell proliferation and apoptosis were detected using MTT assay and flow cytometry, respectively; PARP and PTEN mRNA and protein levels were measured through real-time PCR and western blot assay.
RESULTS AND CONCLUSION: (1) Compared with the model group, the number of tyrosine hydroxylase-positive neurons at the substantia nigra compacta of midbrain was significantly increased in a time-dependant manner in the normal and treatment groups (P < 0.05). (2) The apoptosis rate and mRNA and protein levels of PARP and PTEN in the treatment group were lower than those in the model group. These results suggest that Ginkgo biloba Pingchan Recipe can partly inhibit midbrain neuron apoptosis that may be by decreasing PTEN level. As a result, it becomes possible to prevent and treat Parkinson’s disease via protecting dopamine neurons from loss.

中国组织工程研究杂志出版内容重点：肾移植；肝移植；移植；心脏移植；组织移植；皮肤移植；皮瓣移植；血管移植；器官移植；组织工程

Key words: Drugs, Chinese Herbal, Parkinson Disease, Dopamine, Apoptosis, Tissue Engineering

CLC Number:

R318

Wu Ya-dan, Liang Pei-ri, Long Deng-yi, Gao Bing-miao. Effects of Ginkgo biloba Pingchan Recipe on loss and apoptosis of dopamine neurons in mouse models of Parkinson’s disease[J]. Chinese Journal of Tissue Engineering Research, 2016, 20(49): 7327-7333.

Figures/Tables 3

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