Protective effects of ginsenoside Rb3 on a rat model of ischemia/reperfusion injury

doi:10.3969/j.issn.2095-4344.2016.49.004

Abstract

Abstract:

BACKGROUND: Ginsenoside Rb3 has a variety of physiological activities, which mainly reflected in the cardiovascular treatment.
OBJECTIVE: To study the protective effects of ginsenoside Rb3 on the ischemia/reperfusion injury of rats.
METHODS: 120 Sprague-Dawley rats were randomly assigned to six groups, with 20 in each group. Rats in the model and sham operation groups were intragastrically given physiological saline 2 mL/kg•d for 2 consecutive days. Rats in the positive drug group were intragastrically given diltiazem 2 mL/kg•d for 2 consecutive days. Rats in the low-dose drug group, moderate-dose drug group and high-dose drug group were intragastrically given ginsenoside Rb3 10, 20, 30 mg/kg•d, for 2 consecutive days. At 2 days after administration, the chest of rats in the sham operation was opened, but these rats did not receive any other treatment. In other five groups, anterior descending branch of left coronary artery was ligated to establish models of ischemia/reperfusion injury. 48 hours later, we observed pathological sections of rat cardiac muscle, calculated percentage of organ coefficient and myocardial infarction area, measured the levels of serum isozyme, malondialdehyde, lactate dehydrogenase, endothelial relaxing factor, superoxide dismutase and glutathione peroxidase.
RESULTS AND CONCLUSION: (1) Pathological sections: ginsenoside Rb3 significantly improved the ischemia/reperfusion injury. (2) Percentages of organ coefficient and myocardial infarction area: compared with the model group, the percentages were significantly lower in the moderate-dose and high-dose drug groups (P < 0.05, P < 0.01). (3) Serum indexes: compared with the model group, ginsenoside Rb3 decreased isozyme, malondialdehyde, lactate dehydrogenase and endothelial relaxing factor levels in a dose-dependent manner, but increased superoxide dismutase and glutathione peroxidase levels in a dose-dependent manner. (4) Results suggested that ginsenoside Rb3 has protective effects on ischemia/reperfusion injury. Its mechanism of action may be associated with anti-lipid peroxide activities in cells, the anti-free radical effects, anti-inflammatory functions and the influence of cardiac enzymes on energy metabolism.

中国组织工程研究杂志出版内容重点：肾移植；肝移植；移植；心脏移植；组织移植；皮肤移植；皮瓣移植；血管移植；器官移植；组织工程

Key words: Drugs, Chinese Herbal, Myocardial Ischemia, Myocardial Reperfusion Injury, Tissue Engineering

CLC Number:

R318

Wu Hui-zhen, Jia Qing-zhong. Protective effects of ginsenoside Rb3 on a rat model of ischemia/reperfusion injury[J]. Chinese Journal of Tissue Engineering Research, 2016, 20(49): 7320-7326.

Figures/Tables 2

2.1 实验动物数量分析 120只大鼠均进入结果分析。 2.2 人参皂苷Rb3对大鼠心肌缺血再灌注损伤的影响假手术组心肌组织结构完整，排列整齐，肌原纤维规则，间质可见微量纤维组织，心肌细胞界限清晰，核内物质分布均匀，未见梗死病灶，无大量红细胞和炎细胞浸润；模型组心肌组织出现局部坏死，心肌细胞呈现肿胀，间质增大，边界不清晰，心肌细胞排列紊乱，细胞核不规则，胞浆呈不规则颗粒状，心肌纤维间质有大量红细胞或中性粒细胞浸润；低剂量给药组局部心肌组织出血坏死，心肌细胞结构模糊，界限不清晰；中剂量给药组心肌细胞边界模糊，细胞核聚集，存在炎性细胞浸润；高剂量给药组组心肌细胞完整，排列规整，细胞间质有轻度水肿；阳性药物组心肌细胞完整，排列整齐，细胞间质轻度水肿，见图1。 2.3 人参皂苷Rb3对大鼠心肌梗死面积百分比及脏器系数的影响假手术组中无心肌梗死面积，因此实验结果皆与模型组进行比较。由表1可知，模型组大鼠脏器系数及心肌梗死面积百分比最大，阳性药物组脏器系数及心肌梗死面积百分比最小，两组比较差异有非常显著性意义(P < 0.01)；低剂量给药组脏器系数、梗死面积百分比与模型组比较差异无显著性意义，可认为10 mg/(kg•d)的给药剂量对大鼠心肌梗死面积无显著性影响；中剂量给药组脏器系数与模型组比较差异有显著性意义(P < 0.05)，梗死面积百分比与模型组比较差异有非常显著性意义(P < 0.01)；高剂量给药组脏器系数、梗死面积百分比与模型组比较差异有非常显著性意义(P < 0.01)，提示人参皂苷Rb3在给药剂量为20 mg/(kg•d)与30 mg/(kg•d)能显著降低大鼠心肌梗死面积，降低大鼠心肌缺血再灌注损伤。"

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