Effect of miR-455-3p targeting HIPK2 on proliferation and
apoptosis of osteoblasts induced by high glucose

doi:10.3969/j.issn.2095-4344.2530

Abstract

Abstract:

BACKGROUND: Previous studies have shown that various miRNAs play a role in bone formation. miR-335-5p can protect osteoblasts from oxidative stress and protect osteoblasts under induction with ferric ammonium citrate, but the effect of miR-335-5p on osteoblast proliferation and apoptosis in high glucose environments is unknown.

OBJECTIVE: To investigate the effect of miR-455-3p targeting HIPK2 on proliferation and apoptosis of osteoblasts induced by high glucose.

METHODS: Dual luciferase reporter assay was used to verify the targeting of miR-455-3p to HIPK2. MC3T3-E1 cells were induced by high glucose in vitro, and MC3T3-E1 cells were treated as follows: blank group, high glucose group, high glucose+miR-control group, high glucose+miR-455-3p group, high sugar+si-control group, high sugar+si-HIPK2 group, high glucose+miR-455-3p+pcDNA group and high glucose+miR-455-3p+pcDNA-HIPK2 group. The expression of miR-455-3p and HIPK2 mRNA was detected by qRT-PCR, cell viability was detected by MTT assay, apoptosis was detected by flow cytometry, and the expression of HIPK2, p-STAT3 and STAT3 protein was detected by western blot.

RESULTS AND CONCLUSION: HIPK2 was a target gene of miR-455-3p, and miR-455-3p negatively regulated the expression of HIPK2. High glucose treatment inhibited the expression of miR-455-3p and promoted the expression of HIPK2. The over-expression of miR-455-3p or the inhibition of HIPK2 promoted MC3T3-E1 survival and inhibit cell apoptosis after high glucose treatment. The over-expression of HIPK2 partially reversed the survival promotion and apoptosis inhibition of miR-455-3p on osteoblasts induced by high glucose. miR-455-3p inhibited the expression of p-STAT3 in osteoblasts by regulating HIPK2. To conclude, miR-455-3p inhibits the apoptosis and promotes the proliferation of osteoblasts induced by high glucose via down-regulating HIPK2, which may be related to the inhibition of STAT3 signaling pathway.

Key words: miR-455-3p, HIPK2, high glucose, osteoblasts, apoptosis, STAT3 signaling pathway

CLC Number:

Kuang Jiabing, Shen Wenjuan, Ma Yonggang, Xu Hao, Zhang Keliang, Shen Bo, Xu Yanyan. Effect of miR-455-3p targeting HIPK2 on proliferation and apoptosis of osteoblasts induced by high glucose[J]. Chinese Journal of Tissue Engineering Research, 2020, 24(11): 1641-1646.

Figures/Tables 9

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