Chinese Journal of Tissue Engineering Research ›› 2015, Vol. 19 ›› Issue (46): 7391-7375.doi: 10.3969/j.issn.2095-4344.2015.46.003

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Apoptosis-related genes in human osteoblasts induced by sodium fluoride

Deng Qiang1, Zhang Ya-lou2, Sheng Wei-bin1   

  1. 1Department of Spinal Surgery, the First Affiliated Hospital of Xinjiang Medical University, Urumqi 830011, Xinjiang Uygur Autonomous Region, China; 2Department of Histology and Embryology, Preclinical Institute of Xinjiang Medical University, Urumqi 830011, Xinjiang Uygur Autonomous Region, China
  • Received:2015-09-22 Online:2015-11-12 Published:2015-11-12
  • Contact: Zhang Ya-lou, Ph.D., Associate professor, Department of Histology and Embryology, Preclinical Institute of Xinjiang Medical University, Urumqi 830011, Xinjiang Uygur Autonomous Region, China
  • About author:Deng Qiang, Master, Associate chief physician, Department of Spinal Surgery, the First Affiliated Hospital of Xinjiang Medical University, Urumqi 830011, Xinjiang Uygur Autonomous Region, China
  • Supported by:

    the National Natural Science Foundation of China, No. 81102084, 81460481; the Natural Science Foundation of Xinjiang Uygur Autonomous Region, No. 2015211C040

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Abstract:

BACKGROUND: There are no systematic and coherent studies on the mitochondrial apoptotic pathway of fluoride-induced osteoblast apoptosis, and the specific pathways to induce apoptosis in osteoblasts by fluorine are still unclear.
OBJECTIVE: To explore the possible pathways of apoptosis in osteoblasts induced by fluoride and its molecular characteristics.
METHODS: A fluorosis model of human osteosarcoma cell line Saos-2 was established in vitro. After in vitro culture, the cells were treated with sodium fluoride at different concentrations (0, 5, 10, 20, 40, 80 mg/L). Flow cytometry was used to inspect the mitochondrial membrane potential at 24 hours after intervention; 84 apoptosis-related genes were detected by PCR Array; parts of the differentially expressed genes were verified by western blot method.
RESULTS AND CONCLUSION: When the concentrations of sodium fluoride were 20, 40 and 80 mg/L, the mitochondrial membrane potentials in osteoblasts were 27.0%, 28.8%, 38.6%, respectively (all P < 0.05). PCR  
array found 13 genes upregulated and 15 genes down-regulated. Immunoblotting results showed Bim, Caspase 9, Caspase 14, BCL2, BAX expressions enhanced with increasing doses of sodium fluoride; Caspase 3 expression was decreased at the concentration of 5 mg/L, but increased gradually at over 10 mg/L. Caspase 7 expression had no significant difference. The expression of Caspase 10 decreased with the increasing doses of sodium fluoride. These findings indicate that fluoride may induce apoptosis in osteoblasts through the mitochondrial pathway (including the endoplasmic reticulum stress pathway) and death receptor pathway.
中国组织工程研究杂志出版内容重点：组织构建；骨细胞；软骨细胞；细胞培养；成纤维细胞；血管内皮细胞；骨质疏松；组织工程

Key words: Osteoblasts, Apoptosis, Fluorine, Tissue Engineering