Chinese Journal of Tissue Engineering Research ›› 2015, Vol. 19 ›› Issue (27): 4365-4370.doi: 10.3969/j.issn.2095-4344.2015.27.019

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DNA chip-based gene expression profiling of oral squamous cell carcinoma tissue and normal paracarcinoma tissue

Zheng Jian-wei1, 2, Yang Shu-juan3, Li Xiao-ping2, Wei Cong-yun2, Li Ting2, Mo Wen-juan2, Cai Qiu-yun2, Yang De-qun2, Zhou Lei1, Luo Gang1   

  1. 1Affiliated Stomatology Hospital of Guangdong Province, South Medical University, Guangzhou 510280, Guangdong Province, China; 
    2Shenzhen Dental Medical Center of Guangdong Province, Shenzhen 518001, Guangdong Province, China; 
    3Department of Public Health and Social and Behavioral Sciences, West China School of Public Health, Sichuan University, Chengdu 610041, Sichuan Province, China
  • Online:2015-06-30 Published:2015-06-30
  • Contact: Corresponding author: Luo Gang, M.D., Chief physician, Affiliated Stomatology Hospital of Guangdong Province, South Medical University, Guangzhou 510280, Guangdong Province, China Corresponding author: Zhou Lei, M.D., Chief physician, Affiliated Stomatology Hospital of Guangdong Province, South Medical University, Guangzhou 510280, Guangdong Province, China
  • About author:Zheng Jian-wei, Studying for doctorate, Attending physician, Affiliated Stomatology Hospital of Guangdong Province, South Medical University, Guangzhou 510280, Guangdong Province, China; Shenzhen Dental Medical Center of Guangdong Province, Shenzhen 518001, Guangdong Province, China
  • Supported by:

    Medical Science Research Foundation of Guangdong Province of China, No. A2011092; the National Natural Science Foundation of China, No. 81271159\H1405

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Abstract:

BACKGROUND: In recent years, based on high-throughput molecular imaging, integration of genomics, proteomics and computer aided design and the application of correlative “technical chains” have achieved great achievements in the research of breast cancer, lung cancer, gastric cancer, colon cancer, ovarian cancer and melanin tumor. However, there are few researches on oral squamous cell carcinoma.
OBJECTIVE: To detect the gene expression profile of the oral squamous cell carcinoma tissue and normal paracarcinoma tissue using DNA chip-based gene expression profile.
METHODS: Two samples of oral squamous cell carcinoma tissue and normal paracarcinoma tissue of patients who received treatment at Stomatological Hospital of Guangdong Province of China in 2013 were included in this study. The gene expression profiles of oral squamous cell carcinoma and normal paracarcinoma tissue were determined by the Roche NimbleGen gene expression microarrays.
 
RESULTS AND CONCLUSION: According to screening criteria of differential genes, 7 872 out of 32 448 detected genes were differentially expressed genes of oral squamous cell carcinoma, which accounts for 24% of the total number of the screening genes. 3 800 genes were up-regulated, and 4 072 were down-regulated. The results confirm that through detection with the help of gene expression profile clip, 7 872 differentially expressed genes were obtained through DNA chip-based gene expression profiles according to the screening criteria. Thus it can be concluded that the occurrence and development of the tumors are not a result of single or several genes. Previous experiments based on a single or several genes have great limitations. These findings also suggest that the occurrence of tumor is a result of mutual regulatory effects of many genes forming a network, moreover, the interactions of the network is quite complicated.

中国组织工程研究杂志出版内容重点：肾移植；肝移植；移植；心脏移植；组织移植；皮肤移植；皮瓣移植；血管移植；器官移植；组织工程

Key words: Tissue Engineering, Microchip Analytical Procedures, Oral Spray, Tumor

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