Improved methods of establishing rabbit VX2 hepatocarcinoma models

doi:10.3969/j.issn.2095-4344.2015.27.018

Abstract

Abstract:

BACKGROUND: Rabbit VX2 hepatocarcinoma models are characterized by expanded and infiltrative growth, abundant blood supply, similar pathological and imaging features to human hepatocellular carcinoma, so this model is ideal for imaging experiments.
OBJECTIVE: To establish rabbit VX2 hepatocarcinoma models and compare two different laparotomy methods in establishing rabbit VX2 hepatocarcinoma models.
METHODS: Thirty New Zealand rabbits were randomly divided into two groups: traditional laparotomy (tumor particles were directly embedded into the liver with tweezers) and improved laparotomy (tumor particles were injected into the liver through a 15G syringe needle). The growth features of the tumor established in two different ways were compared by ultrasound.
RESULTS AND CONCLUSION: The tumor formation rate was 100% in the two groups; the single rate was respectively 50% and 90%; the ectopic implantation rate was 50% and 10%, there were significant differences between two groups (P < 0.05). The mortality rate within 2 weeks was 33% and 0 in the two groups, with significant differences (P < 0.05). The improved laparotomy method is feasible for modeling rabbit VX2 hepatocarcinoma, due to minimal injury, short operation time, high success rate and stable features.

中国组织工程研究杂志出版内容重点：肾移植；肝移植；移植；心脏移植；组织移植；皮肤移植；皮瓣移植；血管移植；器官移植；组织工程

Key words: Neoplasms, Models, Animal, Transplantation, Ultrasonography

CLC Number:

R318

Tong Min, Chen Zhu. Improved methods of establishing rabbit VX2 hepatocarcinoma models[J]. Chinese Journal of Tissue Engineering Research, 2015, 19(27): 4361-4364.

Figures/Tables 2

References

[1] 吴发宗,纪建松,涂建飞.兔VX2肝癌模型肝动脉化疗栓塞后微环境中CD3+、CD4+T细胞的表达[J].健康研究,2014,12(15): 622-624.

[2] 孙宏亮,许林锋,唐劲天,等.兔 VX2肝癌模型纳米磁微粒栓塞热疗初步研究[J].中华临床医师杂志,2014,9(8):3328-3329.

[3] Nitta N, Sonoda A, Seko A, et al. A combination of cisplatin-eluting gelatin microspheres and flavopiridol enhances anti-tumour effects in a rabbit VX2 liver tumour model. Br J Radiol. 2010;83:428-432.

[4] Deng J, Virmani S, Yang GY, et al. Intraprocedural diffusion-weighted PROPELLER MRI to guide percutaneous biopsy needle placement within rabbit VX2 liver tumors. J Magn Reson Imaging. 2009;30:366-373.

[5] 钱亭,陈茂振,高峰,等.经肝动脉碘化油/无水乙醇混合剂栓塞消融术治疗兔 VX2肝癌的实验研究[J].介入放射学杂志,2014, 8(23):706-707.

[6] 陈昆涛,何健垣,刘建民.影像实验研究中兔VX2肝癌模型制作的完善及综合评价[J].实用医技杂志,2006,13(20):3607-3610.

[7] 刘鸿,冯巧灵,张玮,等.兔VX2肝癌模型的数字减影血管造影影像分析及改良肝动脉插管术[J].北京大学学报(医学版),2013, 45(4): 649-651.

[8] 王君东,胡锦波,杜伟.改良方法建立兔VX2肝癌模型及其MRI表现[J].医学理论与实践,2013,26(6):701-703.

[9] 杨康健,赵思源,赵斌羽,等.兔肝癌改良接种模型的生长特性研究[J].介入放射学杂志,2010,19(3):211-213.

[10] Matsumua T, Moriyau F, Kono Y, et al. Contrast-enhanced power Doppler imaging of the liver-preliminary animal study. Nippon Rinsho. 1998;56:985-989.

[11] 江翰,王雪峰,王子豪,等.兔VX2肝癌模型的建立及纳米微泡造影剂在模型早期检测中的应用[J].临床肝胆学杂志,2011,27(7): 746-747.

[12] Kievit FM, Veiseh O, Fang C J, et al. Chlorotoxin labeled magnetic nanovectors for targeted gene delivery to glioma. ACS Nano. 2010;4(8):4587-4594.

[13] 王耀普,杨康健,赵思源,等.兔肝癌模型的改良接种及其DSA 影像分析[J].介入放射学杂志,2010,3(19):214-215.

[14] 陈松旺,周云,孟凡荣,等.超声引导下穿刺注射VX2组织块或其悬液制作兔VX2肝癌模型[J].江苏医药,2011,37(2):145-146.

[15] Veiseh M, Gabikian P, Bahrami SB, et al. Cy5.5 bioconjugate for intraoperative visualization of cancer foci. Cancer Res. 2007;67(14):6882-6888.

[16] Veiseh O, kievit FM, Fang CJ, et al. Chlorotoxin bound magnetic nanovector tailored for cancer cell targeting, imaging, and siRNA delivery. Biomaterials. 2010;31(31): 8032-8042.

[17] 刘晓玲,张英娟,李敬东,等.兔VX2肝癌超声造影定量指标与肿瘤微血管密度及血管内皮生长因子的相关性分析[J].生物医学工程与临床,2013,17(2):103-105.

[18] 贾洪顺,全显跃,孙涛.兔VX2肝癌MRI征象与病理对照研究[J].实用放射学杂志,2007,9(23):1254-1256.