Cardiomyocyte apoptosis in rats with myocardial ischemia/reperfusion injury after Exendin-4 pretreatment

doi:10.3969/j.issn.2095-4344.2015.27.005

Abstract

Abstract:

BACKGROUND: Exendin can regulate blood glucose, blood lipid and blood pressure, exert anti-inflammation and anti-oxidative stress effects, improve myocardial infarction and heart failure, and protect heart vessels. However, the effect on the apoptosis of cardiac muscle cells after ischemia/reperfusion injury remains unclear.
OBJECTIVE: To observe the effects of Exendin-4 pretreatment on the cardiomyocyte apoptosis and the expression of Bcl-2 and Bax in rats with myocardial ischemia/reperfusion injury.
METHODS: The myocardial ischemia/reperfusion injury model was established in rats and then received Exendin-4 pretreatment. Ischemia/reperfusion group and sham operation group were set.
RESULTS AND CONCLUSION: Immunohistochemical staining, TUNEL and reverse transcriptase-polymerase chain reaction results showed that Bcl-2 protein and mRNA expression levels in the Exendin-4 group were significantly increased (P < 0.05), while Bax protein and mRNA expression levels were significantly decreased compared with ischemia/reperfusion group (P < 0.05). In addition, apoptosis index was more significantly decreased in the Exendin-4 group than in the ischemia/reperfusion group (P < 0.05). Exendin-4 can protect rat heart muscle against ischemia/reperfusion injury and effectively inhibit the apoptosis of cardiomyocytes, and the underlying mechanism is mediated by up-regulating Bcl-2 expression and down-regulating Bax expression.

中国组织工程研究杂志出版内容重点：肾移植；肝移植；移植；心脏移植；组织移植；皮肤移植；皮瓣移植；血管移植；器官移植；组织工程

Key words: Tissue Engineering, Myocardial Ischemia, Apoptosis, Genes

CLC Number:

R318

Li Wen-kai, Shang Bin, Lian Xiao-peng, Ma Jie. Cardiomyocyte apoptosis in rats with myocardial ischemia/reperfusion injury after Exendin-4 pretreatment [J]. Chinese Journal of Tissue Engineering Research, 2015, 19(27): 4288-4292.

Figures/Tables 5

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