Fasudil inhibits apoptosis of skeletal muscle satellite cells induced by H2O2

doi:10.3969/j.issn.2095-4344.2015.20.008

Abstract

Abstract:

BACKGROUND: Skeletal muscle satellite cells are muscle-derived stem cells with proliferation and differentiation potential distributing between the muscle cell membrane and the base film. Studies have shown that skeletal muscle satellite cells are of efficacy and safety, but the survival rate of the transplanted stem cells is very low, which greatly limits the application of skeletal muscle satellite cells.
OBJECTIVE: To observe the effects of Fasudil on apoptosis of skeletal muscle satellite cells induced by H₂O₂.
METHODS: Skeletal muscle satellite cells cultured in vitro were randomly divided into three groups including H₂O₂group, H2O2+Fasudil group (Fasudil group) and control group. Apoptosis rates were observed by flow cytometry. The concentrations of interleukin-4 and tumor necrosis factor-a in each group were detected by ELISA. Western blot was employed to measure the protein level of Bax in each group.
RESULTS AND CONCLUSION: Compared with the H₂O₂ group, a significant decrease was found in the apoptosis rate of cells, protein level of Bax, and concentrations of interleukin-4 and tumor necrosis factor-a in the Fasudil group (all P < 0.05). These findings indicate that Fasudil can play anti-apoptosis protection by inhibiting Rho-kinase signaling pathway, which may be related to the reduced expression of Bax.

Key words: Hydrogen Peroxide, Satellite Cells, Skeletal Muscle, Apoptosis

CLC Number:

R318

Li Jiang-hua, Dong Shao-hong, Xiong Wei, Liu Qi-yun, Liang Xin-jian, Pang Xin-li . Fasudil inhibits apoptosis of skeletal muscle satellite cells induced by H₂O₂[J]. Chinese Journal of Tissue Engineering Research, 2015, 19(20): 3158-3162.

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References

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Fasudil inhibits apoptosis of skeletal muscle satellite cells induced by H₂O₂

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