Relationships of plasma homocysteine levels and other clinical indexes with the polymorphisms of MTHFR gene C677T among the young people in Shenzhen area

doi:10.3969/j.issn.2095-4344.2014.42.027

Abstract

Abstract:

BACKGROUND: Hyperhomocysteine can be caused by 5,10-methylene tetrahydrofolate reductase (MTHFR) gene mutation, and HHcy is the independent risk factor for cerebral stroke.
OBJECTIVE: To study the correlation between plasma homocysteine level and polymorphisms of MTHFR gene C677T of young people in Shenzhen area, and to explore the relationships of plasma hyperhomocysteine level with other clinical indicators.
METHODS: A total of 101 cases with hyperhomocysteine were collected as experimental group, and 101 cases with normal homocysteine level served as control group (20-45 years old). Genomic DNA was extracted with magnetic nanoparticles method from mouth swab samples of 202 cases. Then the DNA was amplified into target gene fragment by PCR, and amplification product was then sequenced.
RESULTS AND CONCLUSION: The frequencies of CC, CT, TT genotype of MTHFR C677T showed significant differences between the experimental group and the control group (P < 0.01). This evidence indicates that the polymorphisms of MTHFR gene C677T can influence plasma homocysteine level of young people in Shenzhen area; TT genotype frequencies and T allele frequencies in the experimental group were higher than that of control group. Besides, the plasma homocysteine level of TT genotype was significantly higher than that of CT genotype and CC genotype in the experimental group (P < 0.05). We can conclude that TT genotype can improve the homocysteine level more than CT genotype; The systolic blood pressure and diastolic blood pressure in the experimental group were significantly higher than that in the control group (P < 0.05). It indicated that hyperhomocysteine can induce the elevation of blood pressure level; but it is not sure that hyperhomocysteine can increase cholesterol level in our study.

中国组织工程研究杂志出版内容重点：组织构建；骨细胞；软骨细胞；细胞培养；成纤维细胞；血管内皮细胞；骨质疏松；组织工程

全文链接：

Key words: DNA, nano, homocysteine, folic acid

CLC Number:

R318

Bi Jia-jia, Zhang Si-yun, Luo Wen-yuan, Guan Xing-qiu. Relationships of plasma homocysteine levels and other clinical indexes with the polymorphisms of MTHFR gene C677T among the young people in Shenzhen area[J]. Chinese Journal of Tissue Engineering Research, 2014, 18(42): 6871-6877.

Figures/Tables 3

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