Table of Content

08 October 2014, Volume 18 Issue 42 Previous Issue    Next Issue

For Selected:

Download Citations EndNote Reference Manager ProCite BibTeX RefWorks

Toggle Thumbnails

Select

Effect of osteoprotegerin on nitric oxide production and endothelial nitric oxide synthase activity in osteoclasts Zhao Shuai, He Ai-yong, Tao Cheng 2014, 18 (42):  6725-6731.  doi: 10.3969/j.issn.2095-4344.2014.42.001 Abstract ( 268 )   PDF (986KB) ( 440 )   Save BACKGROUND: Osteoprotegerin and nitrogen monoxidum play a key role in the prevention and treatment of osteoporosis. However, the correlation of the two in inhibiting the proliferation and differentiation of osteoclasts remains unclear. OBJECTIVE: To observe the effect of different doses of osteoprotegerin on nitric oxide production and endothelial nitric oxide synthase activity in osteoclasts. METHODS: Tartrate resistant acid phophatase staining was used to test whether the induced cells are osteoclasts. Osteoclasts were divided into six groups: blank control group (no reagent); negative control group (DMEM); four osteoprotegerin groups (10, 25, 50, 75 μg/L of osteoprotegerin). Annexinv-FITC kit and flow cytometry were used to test the apoptosis rate of osteoclasts. Real-time PCR was used to detect the expression  of osteoclasts marker gene, TRAP mRNA and protein kinase K mRNA. Nitric oxide production and nitric oxide synthase activity were determined using the corresponding kits. Four osteoprotegerin groups were added with L-NAME, a kind of nitric oxide synthase inhibitor, to test the changes of the apoptosis rates of osteoclasts and the changes of the expression of TRAP mRNA and protein kinase K mRNA of osteoclasts. RESULTS AND CONCLUSION: Osteoprotegerin inhibited the differentiation of osteoclasts and induced the apoptosis. Osteoprotegerin concentration had a positive correlation with the apoptosis rate of osteoclasts, and a negative correlation with the numbers of osteoclasts and expression of marker gene TRAP mRNA and protein kinase K mRNA in osteoclasts. Osteoprotegerin boosted the nitric oxide production and endothelial nitric oxide synthase activity, and osteoprotegerin concentration was positively correlated to the nitric oxide production and endothelial nitric oxide synthase activity. After Raw264.7 cells were in vitro cultured, osteoprotegerin and nitric oxide play a synergic role in inhibiting osteoclasts production and promoting the apoptosis. We speculate that there is osteoprotegerin/endothelial nitric oxide synthase/nitric oxide signal pathway. 中国组织工程研究杂志出版内容重点：组织构建；骨细胞；软骨细胞；细胞培养；成纤维细胞；血管内皮细胞；骨质疏松；组织工程 全文链接： Figures and Tables | References | Related Articles | Metrics

Select

Puerarin effects on the mRNA expression of osteoblast differentiation-related proteins Yuan Si-yuan, Kong Bei-bei, Sheng Tong, Wang Xin-xiang, Zhang Yun-ling, Liu Xue-mei, Ma Tao, Zheng Hong, Yan Yan, Liu Lian-qi 2014, 18 (42):  6732-6736.  doi: 10.3969/j.issn.2095-4344.2014.42.002 Abstract ( 366 )   PDF (701KB) ( 441 )   Save BACKGROUND: Experimental studies have showed that puerarin has an obvious protective effect on osteoporosis in ovariectomized and orchiectomized mice. But the influence of puerarin in the molecular level in the process of osteoblast differentiation is seldom reported. OBJECTIVE: To observe the effect of puerarin on the mRNA expression of alkaline phosphatase, bone sialoprotein, osteopontin and osteocalcin in osteoblasts. METHODS: The MC3T3-E1 cells from mice cultured in vitro were randomly divided into control group, puerarin group (10-6 mol/L puerarin) and estradiol group (10-7 mol/L estradiol) to observe the effects of puerarin on the differentiation of osteoblasts. mRNA expression of alkaline phosphatase, bone sialoprotein, osteopontin and osteocalcin in MC3T3-E1 cells was determined using RT-PCR method. RESULTS AND CONCLUSION: Puerarin and estradiol both could prolong the expression of alkaline phosphatase that reached the peak at 12 days. Puerarin and estradiol strengthened the mRNA expression of bone sialoprotein at 10 and 12 days, reduced expression of osteopontin at 5 and 12 days, and increased expression of osteocalcin at 10 and 12 days. These results reveal that puerarin can induce the differentiation of  cultured osteoblasts by influencing osteoblast differentiation-related protein mRNA expressions, which may be one of the important molecular mechanisms of puerarin for prevention of osteoporosis. 中国组织工程研究杂志出版内容重点：组织构建；骨细胞；软骨细胞；细胞培养；成纤维细胞；血管内皮细胞；骨质疏松；组织工程 全文链接： Figures and Tables | References | Related Articles | Metrics

Select

Effects of whole-body vertical vibration on the protein expression of FoxO1 in bone tissue and bone marrow cells of ovariectomized osteoporosis rats Wen Si-min, Bu Shu-min, Gao Meng-jin 2014, 18 (42):  6737-6741.  doi: 10.3969/j.issn.2095-4344.2014.42.003 Abstract ( 312 )   PDF (703KB) ( 372 )   Save BACKGROUND: FoxO1 participates in diverse processes of cell physiology, including cell proliferation and apoptosis, reactive oxygen species, longevity, cancer, the regulation of cell cycle and metabolism. Vibration loading is believed to promote metabolism, delay muscle fatigue, and facilitate adaptive bone reconstruction. OBJECTIVE: To investigate the effects of whole-body vertical vibration on the protein expression of FoxO1 in tibia and bone marrow cell of ovariectomized (OVX) osteoporosis rats. METHODS: Thirty-six healthy 3-month-old female Sprague-Dawley rats were randomly divided into the following three groups by body weight: sham-operation (sham) group, OVX group, and OVX whole-body vertical vibration (OVX+V) group. At the 11th week after operation, the OVX+V group rats were vibrated on a vibration platform twice per day for 7 weeks according to the following schedule: whole-body vertical vibration for 15 minutes, with an interval of 10 minutes. The frequency was 90 Hz and the amplitude was 0.5 mm. Within 24-48 hours after the last treatment, the fifth lumbar vertebra as well as the right femur and tibia were isolated. The bone mineral density of the fifth lumbar vertebra and right femur was detected by dual-energy Χ-ray absorptiometry. The FoxO1 protein expression in proximal tibia and bone marrow cells was detected by western blot assay. RESULTS AND CONCLUSION: As revealed by the results of bone mineral density, the bone mineral densities of the proximal and distal femur as well as the fifth lumbar vertebra in OVX+V group were significantly increased while no significant difference could be seen in the mid shaft. As revealed by the results of western blot, there was no FoxO1 protein expression in the tibia, but it could be detected in the bone marrow cells. Compared with the sham group, there was no significant difference in FoxO1 protein expression in bone marrow cells of OVX group; compared with the OVX group, the protein expression level of FoxO1 in bone marrow cells of the OVX+V group was significantly reduced. The results suggested that whole-body vertical vibration could inhibit the protein expression of FoxO1 in bone marrow cells of osteoporosis rats. 中国组织工程研究杂志出版内容重点：组织构建；骨细胞；软骨细胞；细胞培养；成纤维细胞；血管内皮细胞；骨质疏松；组织工程 全文链接： Figures and Tables | References | Related Articles | Metrics

Select

Finite element analysis of calcaneus stress distributions with variant heights Wang Yi-min 2014, 18 (42):  6742-6746.  doi: 10.3969/j.issn.2095-4344.2014.42.004 Abstract ( 347 )   PDF (422KB) ( 722 )   Save BACKGROUND: Calcaneal fractures are often accompanied with a height reduction in the calcaneus. Compared to the intra-articular fracture with much specific operation indications, there is no standard to the reduction of calcaneal height that needs a surgery in extra-articular fractures. Therefore, it is necessary to find a more quantitative operation indication. OBJECTIVE: To explore the changes in calcaneus stress distribution along with the varying calcaneal height through finite element analysis. METHODS: A three-dimensional finite element model of the normal ankle was developed. Four finite element models with the calcaneus height decrease in 5, 10, 15 and 20 mm were developed based on the normal ankle model. And the VonMises distribution of the calcaneus, the maximal stress and its location were respectively analyzed. RESULTS AND CONCLUSION: In the normal ankle model, the stress concentration was found in the posterior inner part of the posterior articular surface, the anterior inner part of the posterior articular facet and the calcaneocuboid articular surface. And the maximal stress was 16.608 MPa, which appeared in the posterior inner part of the posterior articular surface. The stress concentration in the calcaneocuboid articular surface disappeared when the calcaneal height decreased, and the maximal stress of the model with the calcaneal height decrease of 5, 10, 15 and 20 mm was 18.325, 19.674, 22.491 and 25.694 MPa, respectively. The results suggest that the VonMises of calcaneus concentrates and increases obviously when the calcaneal height changes. It seems that the calcaneal height should be restored to the normal level. When the reduction of the fracture is difficult, the decrease of the calcaneal height should be limited within 10 mm. 中国组织工程研究杂志出版内容重点：组织构建；骨细胞；软骨细胞；细胞培养；成纤维细胞；血管内皮细胞；骨质疏松；组织工程 全文链接： Figures and Tables | References | Related Articles | Metrics

Select

Effects of Tougu Xiaotong Capsules on the expression of Rac1 and Cdc42 in chondrocytes Ye Jin-xia, Wu Guang-wen, Li Xi-hai, Zheng Chun-song, Xu Hui-feng, Ye Hong-zhi, Liu Xian-xiang 2014, 18 (42):  6747-6751.  doi: 10.3969/j.issn.2095-4344.2014.42.005 Abstract ( 424 )   PDF (920KB) ( 441 )   Save BACKGROUND: Tougu Xiaotong Capsule has pretty good clinical therapeutic effect on osteoarthritis of early and middle periods. However, the mechanism of Tougu Xiaotong Capsule is not fully clarified. The RhoA GTPases can regulate chondrocyte apoptosis and hypertrophy.  OBJECTIVE: To observe the Tougu Xiaotong Capsule on the expression of Rac1and Cdc42 in tumor necrosis factor-α-induced in vitro cultured rat articular chondrocytes, and to explore its mechanism of action for combating osteoarthritis. METHODS: Knee cartilage of the 4-week-old Sprague-Dawley rats was used to stably establish in vitro culture system of chondrocytes. Passage 3 chondrocytes were identified by toluidine blue staining. Chondrocyte apoptosis was successfully induced by 20 μg/L tumor necrosis factor-α and then Tougu Xiaotong Capsule at different dosage (500, 100, 20 mg/L) was given after 24-hour incubation. MTT assay was used to detect cell survival, flow cytrometry to measure mitochondrial membrane potential, and western blot assay to determine the protein expression of Rac1, Cdc42, Bax and Bcl-2. RESULTS AND CONCLUSION: Tougu Xiaotong Capsule could reduce tumor necrosis factor-α-induced apoptosis of chondrocytes to improve the survival rate of the cells, and at the same time, could down-regulate the protein expression of Rac1, Cdc42 and Bax and increase the protein expression of Bcl-2 significantly (P < 0.05). Tougu Xiaotong Capsule possibly plays a therapeutic efficacy on osteoarthritis by reducing promote apoptosis Rac1, Cdc42 and Bax expression and increasing apoptosis inhibiting gene Bcl-2 expression, thereby to inhibit apoptosis of chondrocytes. 中国组织工程研究杂志出版内容重点：组织构建；骨细胞；软骨细胞；细胞培养；成纤维细胞；血管内皮细胞；骨质疏松；组织工程 全文链接： Figures and Tables | References | Related Articles | Metrics

Select

The interaction between arginase II and microRNA-17 in human pulmonary artery smooth muscle cells Jin You-peng, Pang Ting-ting, Wang Wei, Wang Yu-lin 2014, 18 (42):  6752-6757.  doi: 10.3969/j.issn.2095-4344.2014.42.006 Abstract ( 319 )   PDF (718KB) ( 371 )   Save BACKGROUND: microRNA-17 is confirmed to play an important role in the development of pulmonary hypertension. Some research has shown that hypoxia-induced proliferation in human pulmonary artery smooth muscle cell depends on the induction of arginase II. There is no report about whether there is some interaction between microRNA-17 and arginase II in human pulmonary artery smooth muscle cells. OBJECTIVE: To investigate the possible interactions between microRNA-17 and arginase II in hypoxic human pulmonary artery smooth muscle cells. METHODS: Passage 4 human pulmonary artery smooth muscle cells were cultured in 21% O2 and 5% CO2 (normoxia) or 1% O2 and 5% CO2 (hypoxia), and then transfected with mimic or inhibitor of microRNA-17 or arginase II-small interfering RNA. RNA, microRNA and protein were isolated separately. Expression of microRNA-17 and arginase II was detected with real-time quantitative PCR and western blot assay. RESULTS AND CONCLUSION: The level of microRNA-17 was significantly increased in cultured human pulmonary artery smooth muscle cells exposed to 1% O2 hypoxia, as was arginase II mRNA and protein expression. Furthermore, inhibition of microRNA-17 expression decreased the mRNA and protein levels of arginase II in the human pulmonary artery smooth muscle cells under hypoxia. Conversely, over-expression of microRNA-17 increased the mRNA and protein levels of arginase II in the human pulmonary artery smooth muscle cells under normoxia and hypoxia. Knockdown of arginase II by siRNA abolished the hypoxia-induced up-regulation of microRNA-17 expression. These findings indicate that arginase II is a target gene of microRNA-17 and can regulate the expression of microRNA-17 in human pulmonary artery smooth muscle cells. 中国组织工程研究杂志出版内容重点：组织构建；骨细胞；软骨细胞；细胞培养；成纤维细胞；血管内皮细胞；骨质疏松；组织工程 全文链接： Figures and Tables | References | Related Articles | Metrics

Select

18F-FDG effects on maturation and immune function of dendritic cells in vitro Lei Xiao, Liang Ying-kui, Zhao Wen-rui, Wang Sheng, Guo Feng, Fu Qiang 2014, 18 (42):  6758-6762.  doi: 10.3969/j.issn.2095-4344.2014.42.007 Abstract ( 309 )   PDF (676KB) ( 415 )   Save BACKGROUND: Previous studies have shown that different doses of ionizing radiation may have some impact on maturation and immune function of dendritic cells. OBJECTIVE: To evaluate the effect of 18F-FDG with different concentrations on maturation and immune function of dendritic cells from human peripheral blood mononuclear cells in vitro. METHODS: Human peripheral blood mononuclear cells-derived mature dendritic cells were divided into five groups: PBS, 92.5×104, 185×104, 370×104, 740×104 Bq/mL 18F-FDG were separately added into each group. Dendritic cells were collected 24 hours later. Apoptosis rate, phenotypic expression (CD1α, CD80, CD83, CD86, HLA-DR), ability of mixed lymphocyte reaction and expression of macrophage inflammatory protein 1α and monocyte chemotactic factor-1 in cell culture supernatant were detected. RESULTS AND CONCLUSION: Apoptosis rate, phenotypic expression of CD86, ability of mixed lymphocyte reaction and expression of monocyte chemotactic factor-1 were down-regulated after culture in 740×104 Bq/mL 18F-FDG. However, 18F-FDG at other concentrations had no influence on maturation and immune function of dendritic cells. This study demonstrates that low-concentration 18F-FDG has little influence on apoptosis, maturation, antigen presentation, and migratory capacity of dendritic cells, and it may be selected at an appropriate concentration as a label for dendritic cells in vitro. 中国组织工程研究杂志出版内容重点：组织构建；骨细胞；软骨细胞；细胞培养；成纤维细胞；血管内皮细胞；骨质疏松；组织工程 全文链接： Figures and Tables | References | Related Articles | Metrics

Select

Correlation analysis of pathogenesis of optic neuritis with helper T cell subsets in a mouse experimental autoimmune encephalomyelitis model Yao Han-yun, Wen Fang, Dong Xin-yu 2014, 18 (42):  6763-6768.  doi: 10.3969/j.issn.2095-4344.2014.42.008 Abstract ( 265 )   PDF (820KB) ( 457 )   Save BACKGROUND: More and more evidence have shown that autoimmune-induced inflammatory demyelinating mostly leads to optic neuritis that is quite an early manifestation of multiple sclerosis, but whether the pathogenesis of optic neuritis in experimental autoimmune encephalomyelitis (EAE) mice is correlated with helper T cell subsets has rarely been reported. OBJECTIVE: To analyze the correlation between pathogenesis of optic neuritis of mouse EAE model with helper T cell subsets. METHODS: The mice were injected intraperitoneally Bordetella pertussis to establish EAE models. Then, the animal models were subjected to immunization for 11, 15, 19 days, respectively. Mice undergoing intraperitoneal injection of normal saline served as controls (adjuvant group). RESULTS AND CONCLUSION: Compared with the adjuvant group, the protein expression of interleukin 4 in the optic nerve decreased in the 19-day immunization group (P < 0.05); the protein expression of interleukin 17 in the optic nerve increased in the 11- and 15-day immunization groups (P < 0.05); the protein expression of interferon γ in the optic nerve increased in the 15- and 19-day immunization groups (P < 0.05); the protein expression of Foxp3 in the optic nerve decreased in the 11-, 15- and 19-day immunization groups (P < 0.05). Real-time PCR results showed that compared with the adjuvant group, the mRNA expression of interferon γ and Foxp3 in the optic nerve decreased (P < 0.05), while mRNA expression of RORt increased in the 11-, 15- and 19-day immunization groups; the mRNA expression of interleukin 4, interleukin 17, T-beat increased in the 15- and 19-day immunization groups (P < 0.05); the mRNA expression of GATA3 reduced in the 19-day immunization group (P < 0.05). These results reveal that Foxp3 expression and helper T cell reduction have important influences on the development of optic neuritis in EAE mouse models, interleukin 17 may mediates inflammatory injury in the early stage, while interferon-γ makes inflammatory injury worse in the peak incidence of the disease. 中国组织工程研究杂志出版内容重点：组织构建；骨细胞；软骨细胞；细胞培养；成纤维细胞；血管内皮细胞；骨质疏松；组织工程 全文链接： Figures and Tables | References | Related Articles | Metrics

Select

Expression of recombinant plasmid of homo heterogeneous nuclear ribonucleoprotein E1 in SH-SY5Y cells Huo Li-rong, Wang Lan-ying, Zou Jun-hua, Zhong Nan 2014, 18 (42):  6769-6774.  doi: 10.3969/j.issn.2095-4344.2014.42.009 Abstract ( 269 )   PDF (2344KB) ( 427 )   Save BACKGROUND: The functions of homo heterogeneous ribonucleoprotein E1 are very wide. It can participate in the expression of skeleton proteins in the nervous system. OBJECTIVE: To construct the recombinant plasmid of homo heterogeneous ribonucleoprotein E1 and observe its expression in nerve cells for further studying the functions of it in neurocytes. METHODS: Using pcDNATM4/His C, the homo heterogeneous ribonucleoprotein E1 was subcloned into recombinant plasmid E1-pcDNATM4/His C, followed by enzyming and sequencing. After SH-SY5Y cells were transfected with the recombinant plasmid, western blot analysis and real time RT-PCR were used to detect the expression of homo heterogeneous ribonucleoprotein E1 in SH-SY5Y cells. And the growth of SH-SY5Y cells was observed. RESULTS AND CONCLUSION: We successfully constructed the eukaryotic expressed vector of homo heterogeneous ribonucleoprotein E1. The recombinant plasmids were verified to express in SH-SY5Y cells correctly at mRNA and protein levels. And SH-SY5Y cells generated quickly after homo heterogeneous ribonucleoprotein E1 was over-expressed. The homo heterogeneous ribonucleoprotein E1 is an important protein in neural development. And this vector offers the premise for further studying its function in nervous system. 中国组织工程研究杂志出版内容重点：组织构建；骨细胞；软骨细胞；细胞培养；成纤维细胞；血管内皮细胞；骨质疏松；组织工程 全文链接： Figures and Tables | References | Related Articles | Metrics

Select

Effect of high-fat diet on rabbit knee articular cartilage Wu Song, Wang Xin 2014, 18 (42):  6775-6778.  doi: 10.3969/j.issn.2095-4344.2014.42.010 Abstract ( 340 )   PDF (1581KB) ( 311 )   Save BACKGROUND: The increase of serum cholesterol levels is positively correlated with the increasing incidence of osteoarthritis. Little is known about the role of high-fat diet in the degeneration of articular cartilage and induced arthritis. OBJECTIVE: To observe the morphology change of the articular cartilage of New Zealand rabbits after high-fat diet, and explore the role of food in the articular cartilage degeneration. METHODS: Forty New Zealand white rabbits were randomly divided into two groups. In control group, rabbits were fed with basal diet. In high-fat diet group, rabbits were fed with high-fat diet (20% lard and 80% basal diet). Fasting blood samples was taken every 4 weeks, to detect the triglyceride and total cholesterol levels. After rabbit were fed for 28 weeks, the knee joint was grossly observed and the femoral condyle cartilage was scanned by scanning electron microscope. RESULTS AND CONCLUSION: Compared with the control group, the triglyceride and total cholesterol levels were significantly increased in the high fat group (P < 0.05). Under scanning electron microscope, the high-fat group showed rough surface of condylar cartilage, shallow pouch, messy arrangement, irregular shape and uneven size. The surface of cartilage exhibited focal denudation, irregular arrangement, uneven porosity and fractured cavity. Under higher magnification, small mountain-like structure of cartilage surface became shallow and flattened, the pores disappeared. Long-term high-fat diet may induce and aggravate cartilage damage, suggesting it may be involved in the pathogenesis of osteoarthritis. 中国组织工程研究杂志出版内容重点：组织构建；骨细胞；软骨细胞；细胞培养；成纤维细胞；血管内皮细胞；骨质疏松；组织工程 全文链接： Figures and Tables | References | Related Articles | Metrics

Select

Dibutyl phthalate influences the secretion of rat ovarian granulose cells Wang Xue-jin, Zhou Yun-qing, Luo Xiang-min, Liu Jin 2014, 18 (42):  6779-6783.  doi: 10.3969/j.issn.2095-4344.2014.42.011 Abstract ( 337 )   PDF (697KB) ( 445 )   Save BACKGROUND: Studies have shown that dibutyl phthalate has certain toxicity to the reproductive system of male animals. OBJECTIVE: To investigate the effects of dibutyl phthalate on secretion of rat ovarian granulose cells. METHODS: Ovarian granulosa cells from 25-day-old female Sprague-Dawley rats were cultured in vitro and exposed to different concentrations of dibutyl phthalate (0, 5, 20, 80 μmol/L) for 24 hours. Estradiol and progesterone in ovarian granulosa cells were measured by the method of radio-immunity. Expression of the cytochrome P450aromatase and P450 side chain cleavage enzyme mRNA in granulosa cells were examined by real-time PCR. RESULTS AND CONCLUSION: The levels of estradiol and progesterone in granulosa cells decreased after exposure to dibutyl phthalate. With the increase of the dibutyl phthalate concentration, the expression of P450arom and P450scc mRNA decreased gradually. Dibutyl phthalate could induce reproductive dysfunction and toxicity to female rats. 中国组织工程研究杂志出版内容重点：组织构建；骨细胞；软骨细胞；细胞培养；成纤维细胞；血管内皮细胞；骨质疏松；组织工程 全文链接： Figures and Tables | References | Related Articles | Metrics

Select

Numerical simulation of hemodynamics in the carotid artery Yuan Wei, Chen Zhong-li 2014, 18 (42):  6784-6788.  doi: 10.3969/j.issn.2095-4344.2014.42.012 Abstract ( 376 )   PDF (622KB) ( 679 )   Save BACKGROUND: To establish the real body’s artery model has its own limitations due to the diversity of different individual differences in the carotid artery and blood flow. OBJECTIVE: To study the effect of different inlet velocity profiles of the common carotid artery and three different flow ratios of internal and external carotid artery on hemodynamics of the carotid artery. METHODS: The carotid bifurcation TF-AHCB numerical model was established. Commercial software ANSYS was used to compute the flow field and calculate hematodynamic parameters, including velocity and wall shear stress. Then, their relationship with atherosclerosis was analyzed from different angles. RESULTS AND CONCLUSION: Flow ratio of internal and external carotid artery significantly influences the flow field of the simulated bifurcation of the carotid artery. Changes in the magnitude of inlet velocity cannot only alter the hematodynamic properties, but also change the range of mechanical mechanism. 中国组织工程研究杂志出版内容重点：组织构建；骨细胞；软骨细胞；细胞培养；成纤维细胞；血管内皮细胞；骨质疏松；组织工程 全文链接： Figures and Tables | References | Related Articles | Metrics

Select

A biodynamic experiment of traditional rolling massage and its experimental device development Xu Jun, Li Kai-bin, Qu Qing, Huang Zhong, Zhang Hong 2014, 18 (42):  6789-6794.  doi: 10.3969/j.issn.2095-4344.2014.42.013 Abstract ( 304 )   PDF (1813KB) ( 513 )   Save BACKGROUND: Methods after mechanical stimulation can be combined with various kinds of cells to produce many conversion types so as to start the various physiological functions of the body. OBJECTIVE: To analysis the reason why rolling massage can improve the intracellular concentration of Ca2+. METHODS: A biodynamic experimental device was set up, composed of pressure generating device, pressure forming device, pressure display device and related data recording device. We detected the effects of rolling massage on Ca2+ concentration in normal skeletal muscle cells and damaged cells through the different functions of the experimental device and system software. These functions consisted of pressure display, data transmission and recording, signal acquisition and processing, pressure signal acquisition, control and processing of signal acquisition, wireless signal transmission and receiving system, display system, storage system, system implementation function. RESULTS AND CONCLUSION: The experiment results showed that rolling massage could reduce the Ca2+ concentration in the normal skeletal muscle cells and damaged cells. These findings suggest that rolling massage can improve the overload of intracellular Ca2+ in damaged cells, and it is superior to the sinusoidal mechanical stimulus. 中国组织工程研究杂志出版内容重点：组织构建；骨细胞；软骨细胞；细胞培养；成纤维细胞；血管内皮细胞；骨质疏松；组织工程 全文链接： Figures and Tables | References | Related Articles | Metrics

Select

Generalized design of fitness equipment control interface Lin Neng-tao, Su Zhi-jian, Qiao Shuai, Yan Yang-yi, Yan Yong-jie 2014, 18 (42):  6795-6799.  doi: 10.3969/j.issn.2095-4344.2014.42.014 Abstract ( 371 )   PDF (739KB) ( 574 )   Save BACKGROUND: Health has been paid more and more attention in modern society, the health of those special groups, such as the elderly and the disabled, should also be brought to the forefront. As fitness equipment is more and more popular in contemporary, our focus should be transferred to the use of fitness equipment for these special groups. OBJECTIVE: To put forward the design strategy of fitness equipment control interface, for the special groups to use common fitness equipment comfortably and conveniently. METHODS: Based on the research of existing fitness equipment control interface, according to the usage habits and cognitive awareness of special groups on control interface, a new design strategy has been put forward using the general design method and ergonomics. RESULTS AND CONCLUSION: Based on the existing 45 kinds of exercise bikes, 84 kinds of running machines and 129 kinds of treadmill fitness equipments, we found that these equipments are designed for common users in the aspects of design and production process and control interface. Universal design is a design concept beyond the lack of capacity to meet the different needs of different populations, and designed products can reduce or even eliminate the difference in the ability of special populations and the general population in use. The new strategy makes design of fitness equipment control interface generalized and good man-machine interactive, which can meet the demand of the special groups and help them do fitness exercise to improve their health and body function. 中国组织工程研究杂志出版内容重点：组织构建；骨细胞；软骨细胞；细胞培养；成纤维细胞；血管内皮细胞；骨质疏松；组织工程 全文链接： Figures and Tables | References | Related Articles | Metrics

Select

Hyperbaric oxygen for limb ischemia-reperfusion with no reflow phenomenon: expression of vascular endothelial growth factor and intracellular adhesion molecule 1 Fu Zhao-jun, Xiang Min-hua, Wei Jian, Zhang Chen 2014, 18 (42):  6800-6804.  doi: 10.3969/j.issn.2095-4344.2014.42.015 Abstract ( 273 )   PDF (618KB) ( 394 )   Save BACKGROUND: Clinical limb ischemia-reperfusion with no reflow phenomenon after vascular injury repair seriously affects the prognosis of patients. Therapeutic effect of hyperbaric oxygen in myocardial ischemia reperfusion is exactly confirmed, but hyperbaric oxygen is rarely reported in the treatment of limb ischemia-reperfusion. OBJECTIVE: To detect the expression of vascular endothelial growth factor and intracellular adhesion molecule 1 and to investigate the effect of hyperbaric oxygen on the prognosis of limb ischemia-reperfusion with no reflow phenomenon. METHODS: By clinical screening, cases of main arterial injury of the limbs were selected and subjected to vascular repair for restoring limb blood supply. After surgery, all the cases were randomly divided into two groups (Combined treatment group and surgical group), 16 cases in each group. Combined treatment group was treated with hyperbaric oxygen combined with clinical anticoagulant, antiplatelet treatment; surgical group treated with only postoperative clinical treatment. Another 16 adult healthy volunteers were selected to receive hyperbaric oxygen as hyperbaric oxygen group. Expressions of vascular endothelial growth factor and intracellular adhesion molecule 1 were detected using enzyme-linked immunosorbent assay at 8, 72 hours and 7 days after surgery.  RESULTS AND CONCLUSION: In the combined treatment and surgical groups, patients exhibited higher expressions of vascular endothelial growth factor and intracellular adhesion molecule 1 than those in the hyperbaric oxygen group (P < 0.01). After 72 hours, the expression of vascular endothelial growth factor was significantly higher in the combined treatment group than the surgical group (P < 0.01). At 8 and 72 hours, the expression of intracellular adhesion molecule 1 was lower in the combined treatment group than the surgical group (P < 0.05). These findings suggest that hyperbaric oxygen can induce high expression of vascular endothelial growth factor and inhibit intracellular adhesion molecule 1, which is crucial for improving growth of blood capillary, formation of endothelial cells, reducing no reflow phenomenon. 中国组织工程研究杂志出版内容重点：组织构建；骨细胞；软骨细胞；细胞培养；成纤维细胞；血管内皮细胞；骨质疏松；组织工程 全文链接： Figures and Tables | References | Related Articles | Metrics

Select

Changes of cytokines in the aqueous humor following Avastin intravitreous injection Xiao Jin-an, Bai Qing-yu, Xie An-ming 2014, 18 (42):  6805-6810.  doi: 10.3969/j.issn.2095-4344.2014.42.016 Abstract ( 316 )   PDF (675KB) ( 409 )   Save BACKGROUND: Studies on the inhibitory mechanism of Avastin for proliferative diabetic retinopathy are mostly confined to the vascular endothelial growth factor, while connective tissue growth factor and angiogenesis inhibitor also play an important role in the disease. OBJECTIVE: To evaluate the changes of cytokines in the aqueous undergoing Avastin intravitreous injection or not prior to pars plana vitrectormy in the treatment of proliferative diabetic retinopathy. METHODS: Thirty patients with proliferative diabetic retinopathy (30 eyes) were selected and randomly divided into groups A, B and C, each group containing 10 patients. Group A was treated with single pars plana vitrectormy; and group B and group C had 0.05 mL/1.25 mg and 0.1 mL/1.25 mg Avastin via intravitreous injection at 7 days before pars plana vitrectormy, respectively. Aqueous humor samples were collected during pars plana vitrectormy. The levels of vascular endothelial growth factor, connective tissue growth factor and pigment epithelium-derived factor were assessed by using ELISA technique. RESULTS AND CONCLUSION: The levels of connective tissue growth factor and pigment epithelium-derived factor in the aqueous humor of two Avastin injection groups were increased significantly compared to the group with single pars plana vitrectormy. However, the level of vascular endothelial growth factor was lower in the Avastin injection groups. In addition, there was no significant difference between two Avastin injection groups. These results reveal that intravitreous injection of Avastin assisted vitrectomy for proliferative diabetic retinopathy is more effective than single pars plana vitrectormy treatment. The underlying mechanisms may be that Avastin could inhibit the intraocular angiogenesis by not only reducing the secretion of some growth factors but also increasing the levels of anti-angiogenesis related cytokines. 中国组织工程研究杂志出版内容重点：组织构建；骨细胞；软骨细胞；细胞培养；成纤维细胞；血管内皮细胞；骨质疏松；组织工程 全文链接： Figures and Tables | References | Related Articles | Metrics

Select

Effects of tri-ortho-cresyl phosphate on homeostasis of the glutamate-glutamine cycle and its key enzymes in the brains of hens Zuo En-jun, Jiang Ying, Piao Feng-yuan 2014, 18 (42):  6811-6816.  doi: 10.3969/j.issn.2095-4344.2014.42.017 Abstract ( 330 )   PDF (917KB) ( 373 )   Save BACKGROUND: Although incidents of organophosphate-induced delayed neurotoxicity have been documented for over a century, the molecular mechanisms underlying the axonopathy remain poorly understood. OBJECTIVE: To discuss the effects of tri-ortho-cresyl phosphate (TOCP) on homeostasis of the glutamate-glutamine cycle and the expression of key enzymes in the brains of hens. METHODS: Twenty-four adult hens were randomly divided into three groups (n=8). TOCP group was treated with TOCP by gavage at a single dosage of 1 000 mg/kg, and control group was given an equivalent volume vehicle by gavage, while hens in the phenylmethylsulfonyl fluoride (PMSF)+TOCP group were subcutaneously injected with   40 mg/kg PMSF followed by 1 000 mg/kg TOCP 24 hours later. The hens were killed on days 5 and 21 post-dosing. The brains were taken out quickly and preserved in a -80 ℃ deep freezer. ELISA was used to determination the content of glutamine synthetase and glutaminase and the activity of glutamine synthetase. Corresponding kits were used to measure the level of glutamate and glutamine. Fluo3-AM was used to measure cytosolic calcium concentration. RESULTS AND CONCLUSION: The activity and content of glutamine synthetase and the concentration of glutamine were down-regulated, while the concentrations of the intracellular Ca2+ and glutamate were up-regulated in the early stage after TOCP administration. It is also suggested that the downregulated expression of glutamine synthetase may be associated with organophosphate-induced delayed neurotoxicity through the disruption of homeostasis of the glutamate-glutamine cycle and cytosolic calcium concentration. 中国组织工程研究杂志出版内容重点：组织构建；骨细胞；软骨细胞；细胞培养；成纤维细胞；血管内皮细胞；骨质疏松；组织工程 全文链接： Figures and Tables | References | Related Articles | Metrics

Select

Comparison of single-bundle and double-bundle autografts in anterior cruciate ligament reconstruction Tan Jie, Feng Xiao-bing, Qu Jian-hua, Wu Chang-jie, Zhao Xin-dong, Li Ying-xiang, Yan Bang-kai 2014, 18 (42):  6817-6821.  doi: 10.3969/j.issn.2095-4344.2014.42.018 Abstract ( 507 )   PDF (575KB) ( 561 )   Save BACKGROUND: Different weaving methods of autologous tendon lead to various treatment efficacies on cruciate ligament rupture, but the preferred method is still controversial. OBJECTIVE: To comparatively analyze the clinical efficacy of single-bundle and double-bundle autografts on anterior cruciate ligament reconstruction. METHODS: A retrospective analysis of 48 cases of anterior cruciate ligament rupture was performed. According to the composition of graft beam, these 48 patients were divided into two groups: double-bundle group (n=24) and single-bundle group (n=24). All surgical patients underwent autologous anterior cruciate ligament reconstruction by arthroscopy, and were followed up for at least 6 months. The function of knee joint after operation was comprehensively analyzed through IKDC and Lysholm scores. RESULTS AND CONCLUSION: All of the patients (n=48) exhibited no joint complications postoperatively, such as intra-articular infection, joint effusion and incision inflammation. The IKDC scores and Lysholm scores in the double-bundle group were better than those in the single-bundle group, but there was no statistically significant difference (P > 0.05). This study demonstrated that single-bundle and double-bundle autografts both have good curative effects on anterior cruciate ligament reconstruction by arthroscopy. But in contrast, the curative effects of double-bundle autografts are better. 中国组织工程研究杂志出版内容重点：组织构建；骨细胞；软骨细胞；细胞培养；成纤维细胞；血管内皮细胞；骨质疏松；组织工程 全文链接： Figures and Tables | References | Related Articles | Metrics

Select

Mid-term outcome of medial patellofemoral ligament reconstruction by hamstring tendon autograft Ren Yi-zhong, Han Chang-xu, Jia Yan-bo, Kong Ling-yue, Eerguotu 2014, 18 (42):  6822-6826.  doi: 10.3969/j.issn.2095-4344.2014.42.019 Abstract ( 311 )   PDF (664KB) ( 354 )   Save BACKGROUND: Autologous hamstring tendon reconstruction of the medial patellofemoral ligament is now more popular surgical procedure. OBJECTIVE: To study the clinical mid-term outcomes of isolated medial patellofemoral ligament reconstruction for recurrent lateral patellar dislocation. METHODS: The clinical data of 24 patients (25 knees) undergoing medial patellofemoral ligament reconstruction for recurrent patellar dislocation from September 2006 to September 2009 were retrospectively studied. Clinical, functional, and CT outcomes were assessed at an average of 3.1 years after surgery (range, 2-5 years), using recurrent instability as the primary end point. RESULTS AND CONCLUSION: The success rate of medial patellofemoral ligament reconstruction for preventing recurrent dislocations was 92%. Two patients (8%) experienced a recurrent lateral patellar dislocation, and required a reoperation. At final follow-up, the mean IKDC, Tenger and Lycholm scores were significantly higher than those before operation (P < 0.01). Reconstruction of the medial patellofemoral ligament for recurrent patellar instability is a minimally invasive operation and has a number of benefits. The mid-term clinical outcome was satisfactory. 中国组织工程研究杂志出版内容重点：组织构建；骨细胞；软骨细胞；细胞培养；成纤维细胞；血管内皮细胞；骨质疏松；组织工程 全文链接： Figures and Tables | References | Related Articles | Metrics

Select

Biomechanical characteristics of suturing the ligament end during anterior cruciate ligament reconstruction Chen Xiao, Huang Jing-min 2014, 18 (42):  6827-6831.  doi: 10.3969/j.issn.2095-4344.2014.42.020 Abstract ( 323 )   PDF (607KB) ( 670 )   Save BACKGROUND: During autologous tendon grafting, the ultimate tensile strength used for suturing the end of the ligament is important for successful surgery. Improving suturing strength and increasing the number of stitches is a good choice for increasing the fixed intensity. But excess amount of stitches can produce too many thread residues, thereby affecting tendon healing. OBJECTIVE: To investigate the essential number of suturing pins for the anterior cruciate ligament revascularization in ligament end suture fixation to reduce suturing thread exposure. METHODS: (1) In vitro biomechanics test: 12 patellar ligament specimens were divided into two groups: the specimens were sutured with 5 or 3 stitches using Krackow suture method. The suturing thread was J&J tendon suture thread. The strength of tensile was compared between the two groups by Tensile mechanical test was conducted to compare the strength of tensile between the two groups and to explore the optimal number of stitches and suturing method. (2) Clinical application: According to the results of in vitro experiments, modified Krackow suture method was used clinically for arthroscopic anterior cruciate ligament reconstruction in 125 cases, including 62 cases receiving 3-stitch suture, and 63 cases undergoing 5-stitch suture.  RESULTS AND CONCLUSION: The fixed strength of tensile at suture sites was over 100 N for 3-stitch  double-lock suture and 110 N for 5-stitch double-lock suture. There was no significant difference in the fixed strength between the two groups, but their strength values were both over the breaking strength of suturing materials. Moreover, the fixed strength could not be reduced by suturing throughout the ligament at the first stitch. All the 125 cases were followed for 6.4 months averagely, and both 3-stitch and 5-stitch suture methods achieved good outcomes. The satisfaction rate was up to 99%, and no suture breakage or loosing occurred at early and late stages. These findings suggest that, using 3-stitch double-lock suture method, a satisfactory fixed strength can be achieved with reduced thread exposure. The suturing thread can run through the ligament at the first stitch, which can reduce thread exposure but not reduce the fixed strength. 中国组织工程研究杂志出版内容重点：组织构建；骨细胞；软骨细胞；细胞培养；成纤维细胞；血管内皮细胞；骨质疏松；组织工程 全文链接： Figures and Tables | References | Related Articles | Metrics

Select

Perioperative management in traumatic soft tissue defects with revascularized flaps Zhang Gui-chun, Cao Xue-cheng, Zheng Jin-long 2014, 18 (42):  6832-6837.  doi: 10.3969/j.issn.2095-4344.2014.42.021 Abstract ( 223 )   PDF (436KB) ( 516 )   Save BACKGROUND: Traumatic soft tissue defects of the limbs are usually accompanied with the exposure of tendon, joint capsule, bone or internal fixator, which can be reconstructed by skin flap. Previous research has shown that it is an important method to repair traumatic tissue defects with flaps. However, rarely research reports perioperative management about flap bed so far. OBJECTIVE: To explore the perioperative strategy for repairing traumatic soft tissue defects with revascularized flaps. METHODS: Totally 94 cases undergoing secondary skin flap repair were enrolled. Intraoperative debridement using tourniquet was performed, and the wound was washed with mass of physiological saline. Whether the tissues, including bone, tendon, joint capsule and internal fixator, were reserved or not depended on their viability, and then the flaps were harvested to repair defects, and drainage was placed properly at last. RESULTS AND CONCLUSION: The flaps survived in all cases. Exudation occurred in 5 cases with the exposure of bone, and 28 cases with the exposure of tendon or joint capsule healed normally. No complications were associated with the reservation of the internal fixators, but delayed-union occurred in three cases and nonunion in one case. These findings indicate that the perioperative treatment of the application of skin flap is worthy of attention. Careful debridement, advisable choice of the flap, efficient drainage and using antibiotic normatively are all keys. Treatment of the bone, tendon, joint capsule and internal fixator which are exposed should not only weigh the advantage and disadvantage, but also relax the indication of reserving them. 中国组织工程研究杂志出版内容重点：组织构建；骨细胞；软骨细胞；细胞培养；成纤维细胞；血管内皮细胞；骨质疏松；组织工程 全文链接： Figures and Tables | References | Related Articles | Metrics

Select

Effects of exercise on bone turnover: promoting or inhibiting development and viability of osteoblasts/osteoclasts Zhang Ling-li, Chen Bing-lin, Zou Jun 2014, 18 (42):  6838-6843.  doi: 10.3969/j.issn.2095-4344.2014.42.022 Abstract ( 445 )   PDF (692KB) ( 823 )   Save BACKGROUND: Bone turnover markers are the products in the blood or urine in the process of bone reconstruction, which can be used to evaluate bone resorption and bone formation rate, thereby indicating potential bone diseases. OBJECTIVE: To analyze the effect of exercise on the levels of bone turnover markers in the blood and urine samples. METHODS: Databases of PubMed and Wangfang were retrieved with key words of “bone formation, bone resorption, alkaline phosphotase, osteocalcin PICP, PINP, hydroxyproline, pyridinoline, tartrate resistant acid phosphatase” by screening titles and abstracts to search papers related to exercise effects on the biological markers of bone formation. Finally, 48 papers were discussed. RESULTS AND CONCLUSION: The effect of exercise on bone turnover refers to the influence on bone formation and bone resorption. Exercise enhances or inhibits the activity of osteoblasts and osteoclasts, and accelerates or delays bone reconstruction by promoting or inhibiting the growth of osteoblasts and osteoclasts. In recent years, exercise exhibits more and more influence on the bone. Bone turnover markers compared with bone mineral density show changes earlier. By measuring the levels of bone turnover markers in blood and urine samples, it help us to understand the metabolism of bone tissue, to evaluate bone metabolic state, osteoporosis diagnosis classification, predicting of fracture risk, to observe the curative effect of drug treatment, and to diagnosis the metabolic bone differential disease. 中国组织工程研究杂志出版内容重点：组织构建；骨细胞；软骨细胞；细胞培养；成纤维细胞；血管内皮细胞；骨质疏松；组织工程 全文链接： Figures and Tables | References | Related Articles | Metrics

Select

Pathological mechanics and treatment of plantar fasciitis: future prospects of platelet-rich plasma therapy Pan Zhang-yi, Yin Feng 2014, 18 (42):  6844-6848.  doi: 10.3969/j.issn.2095-4344.2014.42.023 Abstract ( 360 )   PDF (719KB) ( 552 )   Save BACKGROUND: The treatments of plantar fasciitis as an important cause of calcaneodynia are different in medical institutions, as well as the therapeutic results. OBJECTIVE: To summarize various treatments of plantar fascia, and based on the pathogenesis and pathological mechanics of plantar fasciitis, to investigate the effect of various treatment methods and indications. METHODS: A computer-based online search of PubMed database and Wanfang database was performed for articled related to plantar fasciitis published between January 1993 and January 2014. The keywords were “plantar fasciitis, mechanism, treatment” in English and Chinese, respectively, by screening the titles and abstracts. Finally, 34 articles were included in result analysis. RESULTS AND CONCLUSION: The treatment of plantar fasciitis needs to follow the principle from noninvasive to invasive treatment. Stretching the plantar fascia and heel cord, using prefab-ricated orthotics, and wearing night splints are crucial for the treatment of plantar fasciitis. Local hormone injection can be used as first-line therapy for patients with acute plantar fasciitis if they can tolerate the adverse reactions due to hormone therapy. Platelet-rich plasma therapy has a promising prospect, but there is lack of experimental evidences. Extracorporeal shock wave therapy can be a choice for recurrent chronic intractable plantar fasciitis. Surgical intervention remains the last line of therapy, for which, rigorous screening is important, but it is not always effective at reducing pain. 中国组织工程研究杂志出版内容重点：组织构建；骨细胞；软骨细胞；细胞培养；成纤维细胞；血管内皮细胞；骨质疏松；组织工程 全文链接： Figures and Tables | References | Related Articles | Metrics

Select

Assessment for early embryo quality improves the sensitivity and specificity to predicting embryonic development potential Li Nan, Li Jing-yu, Tang Yong-mei, Wei Ji-hong 2014, 18 (42):  6849-6855.  doi: 10.3969/j.issn.2095-4344.2014.42.024 Abstract ( 336 )   PDF (624KB) ( 1199 )   Save BACKGROUND: Embryo viability assessment is directly related to the selection of embryo transplantation and clinical outcome of assisted reproduction. OBJECTIVE: To summarize the assessment methods for early embryonic development. METHODS: The first author searched PubMed database for relevant articles published from January 1990 to December 2013 using the keywords of “assisted reproductive technology, art; pre-implantation embryo; embryonic development viability; evaluation methods” in English. Finally, 63 articles were included in result analysis. RESULTS AND CONCLUSION: In the embryo quality evaluation, the most widely used method is morphological evaluation method which is characterized as rapid, non-invasive, and simple. With the development of assisted reproductive technology, the morphological evaluation combined with time-lapse imaging analysis system has been recognized in embryo selection. Recently, targeted-metabolic analysis has been proposed as a useful tool for assessment of embryo development potential, involving pyruvate acid, glycometabolism, amino acid, and embryo-derived cytokines (soluble human leukocyte antigen G1, platelet-activating factor, etc.). Furthermore, the pre-implantation genetic screening method targeting gene and chromosome abnormality is expected to find more effective markers for evaluating embryo developmental potential. 中国组织工程研究杂志出版内容重点：组织构建；骨细胞；软骨细胞；细胞培养；成纤维细胞；血管内皮细胞；骨质疏松；组织工程 全文链接： Figures and Tables | References | Related Articles | Metrics

Select

Research front and hotspots of neuronal calcium sensor-1 Zhu Yu-zhen, Zhang Qing-wen 2014, 18 (42):  6856-6862.  doi: 10.3969/j.issn.2095-4344.2014.42.025 Abstract ( 341 )   PDF (682KB) ( 453 )   Save BACKGROUND: Research front and hotspots of neuronal calcium sensor-1 are always the focus for the researchers in this field. OBJECTIVE: To probe the research front and hotspots of neuronal calcium sensor-1 with the methods of quantitative analysis. METHODS: The methods of co-cited articles analysis and word frequency analysis were used in the article. The objects were 363 articles from Web of Science by US Institute for Scientific Information (ISI) about the neuronal calcium sensor-1 from 1982 to 2014. The network of co-cited articles and keywords was showed in visualization mapping by using CiteSpace III in which the burst nodes represented the high impact hot papers and the most frequently used keywords, and revealed the research frontier and the hot spots of neuronal calcium sensor-1. RESULTS AND CONCLUSION: The physiological functions of neuronal calcium sensor-1 are the research frontier and the hot spots. The transformational point in time spot of the hotspots is during 1994 to 1996, 2000, 2008, 2012; and the different research focus showed in each stage: the structure and characterization of the protein during 1992-2000 and the protein function and the role during 2004-2012 are the research hotspots, while during 2008-2014 the hotspots place extra emphasis on the higher function (e.g. memory) and several diseases(such as schizophrenia, cancer, autism, depression, senile dementia, neuron damage, etc). The determination of the research frontier domains and hot spots of neuronal calcium sensor-1 will indicate the goal and direction for the further studies. 中国组织工程研究杂志出版内容重点：组织构建；骨细胞；软骨细胞；细胞培养；成纤维细胞；血管内皮细胞；骨质疏松；组织工程 全文链接： Figures and Tables | References | Related Articles | Metrics

Select

Autograft versus non-irradiated allograft for anterior cruciate ligament reconstruction: a meta-analysis Wang Yi-lun, Xie Dong-xing, Li Hui, Yang Tuo, Deng Zhen-han, Yang Ye, Zhang Yi, Ding Xiang, Lei Guang-hua 2014, 18 (42):  6863-6870.  doi: 10.3969/j.issn.2095-4344.2014.42.026 Abstract ( 289 )   PDF (717KB) ( 465 )   Save BACKGROUND: Reconstruction of the anterior cruciate ligament has become the gold-standard treatment for an anterior cruciate ligament rupture. Despite the popularity of the procedure, there remains a considerable amount of controversies over whether an autograft or anallograft should be used for primary anterior cruciate ligament reconstruction. OBJECTIVE: To compare the clinical outcomes of allograft and autograft in primary anterior cruciate ligament reconstruction. METHODS: Randomized controlled trials concerning autograft versus non-irradiated allograft for anterior cruciate ligament reconstruction were identified from the PubMed/Medline database conducted up to July 12, 2014. These studies were selected independently by two reviewers according to the inclusion and exclusion criteria. Meta-analysis was performed using Revman5.2 software. RESULTS AND CONCLUSION: A total of 6 randomized controlled trials consisting of 858 cases were included, 441 cases in autograft group and 417 cases in non-irradiated allograft. The results of the meta-analysis indicate no significant difference between autografts and non-irradiated allografts in overall IKDC [relative risk (RR)=1.02, 95% confidence interval (CI) (0.99 to 1.06), P=0.21], degree of laxity [mean difference (MD)=-0.13, 95% CI (-0.29 to -0.02), P=0.09], Lachman test [RR=1.04, 95% CI (0.95 to 1.13), P=0.37], pivot shift test [RR=1.00, 95% CI (0.95 to 1.05),P=0.96], one-leg hop test [RR=1.01, 95% CI (0.96 to 1.06), P=0.77], Lysholm score [MD=-0.64, 95% CI (-1.45 to 0.17), P=0.12], Tegner score [MD=0.16, 95% CI (-0.16 to 0.47), P=0.34] and rate of postoperative complications [RR=1.42, 95% CI (0.67 to 3.04), P=0.36]. Therefore, in the meta-analysis, there is no significant difference between autograft and non-irradiated allograft in clinical outcomes. However due to the limitations of our study, further work is needed to determine this conclusion. 中国组织工程研究杂志出版内容重点：组织构建；骨细胞；软骨细胞；细胞培养；成纤维细胞；血管内皮细胞；骨质疏松；组织工程 全文链接： Figures and Tables | References | Related Articles | Metrics

Select

Relationships of plasma homocysteine levels and other clinical indexes with the polymorphisms of MTHFR gene C677T among the young people in Shenzhen area Bi Jia-jia, Zhang Si-yun, Luo Wen-yuan, Guan Xing-qiu 2014, 18 (42):  6871-6877.  doi: 10.3969/j.issn.2095-4344.2014.42.027 Abstract ( 334 )   PDF (872KB) ( 448 )   Save BACKGROUND: Hyperhomocysteine can be caused by 5,10-methylene tetrahydrofolate reductase (MTHFR) gene mutation, and HHcy is the independent risk factor for cerebral stroke. OBJECTIVE: To study the correlation between plasma homocysteine level and polymorphisms of MTHFR gene C677T of young people in Shenzhen area, and to explore the relationships of plasma hyperhomocysteine level with other clinical indicators. METHODS: A total of 101 cases with hyperhomocysteine were collected as experimental group, and 101 cases with normal homocysteine level served as control group (20-45 years old). Genomic DNA was extracted with magnetic nanoparticles method from mouth swab samples of 202 cases. Then the DNA was amplified into target gene fragment by PCR, and amplification product was then sequenced. RESULTS AND CONCLUSION: The frequencies of CC, CT, TT genotype of MTHFR C677T showed significant differences between the experimental group and the control group (P < 0.01). This evidence indicates that the polymorphisms of MTHFR gene C677T can influence plasma homocysteine level of young people in Shenzhen area; TT genotype frequencies and T allele frequencies in the experimental group were higher than that of control group. Besides, the plasma homocysteine level of TT genotype was significantly higher than that of CT genotype and CC genotype in the experimental group (P < 0.05). We can conclude that TT genotype can improve the homocysteine level more than CT genotype; The systolic blood pressure and diastolic blood pressure in the experimental group were significantly higher than that in the control group (P < 0.05). It indicated that hyperhomocysteine can induce the elevation of blood pressure level; but it is not sure that hyperhomocysteine can increase cholesterol level in our study. 中国组织工程研究杂志出版内容重点：组织构建；骨细胞；软骨细胞；细胞培养；成纤维细胞；血管内皮细胞；骨质疏松；组织工程 全文链接： Figures and Tables | References | Related Articles | Metrics

Select

Digit and digit ratio of Xinjiang young Kazakh nationality Nuermaimaiti Bahaxiaer, Ai Hua, Wu Hai-zhong 2014, 18 (42):  6878-6883.  doi: 10.3969/j.issn.2095-4344.2014.42.028 Abstract ( 327 )   PDF (654KB) ( 440 )   Save BACKGROUND: Digit ratio is basically determined within the first 3 months of fetal development. Digit and digit ratio exhibit differences between genders and between left and right hands. Nationality, regional and ethnic difference may also influence the digit ratio. So far, there is no report about the digit ratio of Kazakh adults. OBJECTIVE: To study the characteristics of digit ratio of young Kazakh nationality in Xinjiang of China. METHODS: A total of 200 young Kazakh volunteers in Xinjiang, aged 18-25 years, were enrolled, including   100 males and 100 females. These participants were subjected to posteroanterior X-ray of both hands. Phalanx length and body height were measured, and the resulting data were statistically analyzed using SPSS19.0 software. RESULTS AND CONCLUSION: The digit ratio of Xinjiang Kazakh people was 3D > 4D > 2D > 5D. Male digit ratio possessed the tendency of 3D:5D > 4D:5D > 2D:5D > 3D:4D > 3D:4D > 2D:3D > 2D:4D; female digit ratio possessed the tendency of 3D:5D > 2D:5D > 3D:4D > 4D:5D > 2D:4D > 2D:3D. The digit ratio of 2D:3D, 3D:4D, 4D:5D was higher in the male than the female; the digit ratio of 2D:4D, 2D:5D, 3D:5D was higher in the female than the male. Digit ratio had sexual differences, especially in 3D:5D (P < 0.05), and had bilateral differences, especially in 2D:4D and 4D:5D (P < 0.05). These findings indicate that the digit ratio of Xinjiang Kazakh people exhibits a bilateral difference, especially in 2D:4D and 4D:5D, as well as a sexual difference, especially in 3D:5D. 中国组织工程研究杂志出版内容重点：组织构建；骨细胞；软骨细胞；细胞培养；成纤维细胞；血管内皮细胞；骨质疏松；组织工程 全文链接： Figures and Tables | References | Related Articles | Metrics

Select

Expression of galectin-7 in acute antibody-mediated rejection after renal transplantation Han Yong, Guo Hui, Cai Ming, Qian Ye-yong, Li Zhou-li, Zhou Wen-qiang, Wang Qiang, Xu Xiao-guang, Huang Hai-yan, Xiao Li, Wang Xin-ying, Shi Bing-yi 2014, 18 (42):  6884-6888.  doi: 10.3969/j.issn.2095-4344.2014.42.029 Abstract ( 261 )   PDF (655KB) ( 370 )   Save BACKGROUND: The role of galactose lectin family proteins in transplantation immunity has been proposed, but there is currently no galectin-7 detection for auxiliary diagnosis of renal dysfunction in the perioperative period after renal transplantation. For renal transplant recipients, monitoring of galectin-7 may contribute to early diagnosis of renal dysfunction after renal transplantation, and buy time for clinical treatment. OBJECTIVE: To detect the expression of galactose-7 in acute antibody mediated rejection after renal transplantation. METHODS: Twenty-seven patients who were diagnosed as having acute antibody mediated rejection after renal transplantation by renal biopsy were enrolled, and another 10 patients without acute antibody mediated rejection after renal transplantation were selected as controls. Immunohistochemical staining and western blot assay were used to detect expression of galectin-7 in tissue and serum, respectively. RESULTS AND CONCLUSION: Results of immunohistochemistry staining showed that under light microscope, in the control group, galectin-7 distributed in the surface microvilli of proximal tubule epithelial cells, but not in glomeruli, distal tubule, collecting duct and vein; in the acute rejection group, renal arteriole intima edema, tube wall fibrinoid necrosis, infiltration of renal glomerulus and tubule cells and mononuclear cells were found and galectin-7 only expressed in the surface microvilli of proximal tubule epithelial cells as well as in the arterial smooth muscle. The number of galectin-7 positive cells in the acute rejection group was significantly higher than that in the control group (P < 0.1). Western blot assay results showed that the protein expression of serum galectin-7 in the acute rejection group was higher than that in the control group (P < 0.05). These findings indicate that renal puncture for renal transplantation is safe and reliable, has no adverse effect on the patients and renal transplant. Galectin-7 detection has an important guiding significance for the auxiliary diagnosis of renal dysfunction during the perioperative period after renal transplantation. 中国组织工程研究杂志出版内容重点：组织构建；骨细胞；软骨细胞；细胞培养；成纤维细胞；血管内皮细胞；骨质疏松；组织工程 全文链接： Figures and Tables | References | Related Articles | Metrics