Effects of whole-body vertical vibration on the protein expression of FoxO1 in bone tissue and bone marrow cells of ovariectomized osteoporosis rats

doi:10.3969/j.issn.2095-4344.2014.42.003

Abstract

Abstract:

BACKGROUND: FoxO1 participates in diverse processes of cell physiology, including cell proliferation and apoptosis, reactive oxygen species, longevity, cancer, the regulation of cell cycle and metabolism. Vibration loading is believed to promote metabolism, delay muscle fatigue, and facilitate adaptive bone reconstruction.
OBJECTIVE: To investigate the effects of whole-body vertical vibration on the protein expression of FoxO1 in tibia and bone marrow cell of ovariectomized (OVX) osteoporosis rats.
METHODS: Thirty-six healthy 3-month-old female Sprague-Dawley rats were randomly divided into the following three groups by body weight: sham-operation (sham) group, OVX group, and OVX whole-body vertical vibration (OVX+V) group. At the 11th week after operation, the OVX+V group rats were vibrated on a vibration platform twice per day for 7 weeks according to the following schedule: whole-body vertical vibration for 15 minutes, with an interval of 10 minutes. The frequency was 90 Hz and the amplitude was 0.5 mm. Within 24-48 hours after the last treatment, the fifth lumbar vertebra as well as the right femur and tibia were isolated. The bone mineral density of the fifth lumbar vertebra and right femur was detected by dual-energy Χ-ray absorptiometry. The FoxO1 protein expression in proximal tibia and bone marrow cells was detected by western blot assay.
RESULTS AND CONCLUSION: As revealed by the results of bone mineral density, the bone mineral densities of the proximal and distal femur as well as the fifth lumbar vertebra in OVX+V group were significantly increased while no significant difference could be seen in the mid shaft. As revealed by the results of western blot, there was no FoxO1 protein expression in the tibia, but it could be detected in the bone marrow cells. Compared with the sham group, there was no significant difference in FoxO1 protein expression in bone marrow cells of OVX group; compared with the OVX group, the protein expression level of FoxO1 in bone marrow cells of the OVX+V group was significantly reduced. The results suggested that whole-body vertical vibration could inhibit the protein expression of FoxO1 in bone marrow cells of osteoporosis rats.

中国组织工程研究杂志出版内容重点：组织构建；骨细胞；软骨细胞；细胞培养；成纤维细胞；血管内皮细胞；骨质疏松；组织工程

全文链接：

Key words: vibration, ovariectomy, osteoporosis, postmenopausal

CLC Number:

R318

Wen Si-min, Bu Shu-min, Gao Meng-jin. Effects of whole-body vertical vibration on the protein expression of FoxO1 in bone tissue and bone marrow cells of ovariectomized osteoporosis rats[J]. Chinese Journal of Tissue Engineering Research, 2014, 18(42): 6737-6741.

Figures/Tables 1

References

[1] Levine JP.Pharmacologic and nonpharmacologic management of osteoporosis. Clin Cornerstone.2006;8 (1): 40-53.
[2] McClung MR, Balske A, Burgio DE, et al. Treatment of postmenopausal osteoporosis with delayed-release risedronate 35 mg weekly for 2 years. Osteoporos Int. 2013; 24(1):301-310.
[3] Chen Yi, Yin Zhixun, Li Zhichen, et al. Risk assessment of osteoporotic fracture.Chin J Joint Surg.2012;6(4):643-647.
[4] Brunet A,Sweeney LB,Sturgill JF,et al.Stress-dependent regulation of FOXO transcription factors by the SIRT1 deacetylase.Science.2004;303 (5666):2011-2015.
[5] Moriishi T, Kawai Y, Komori, et al.Bcl2 deficiency activates FoxO through Akt inactivation and accelerates osteoblast differentiation. PLoS One.2014;9(1): e86629.
[6] Iyer S,Ambrogini E,Bartell SM,et al.FOXOs attenuate bone formation by suppressing Wnt signaling.J Clin Invest. 2013; 123 (8):3409-3419.
[7] 王兴泽.振动负荷训练研究进展[J].中国运动医学杂志, 2012, 31(7):648-653.
[8] Tzivion G, Dobson M, Ramakrishnan G. FoxO transcription factors; Regulation by AKT and 14-3-3 proteins. Biochim Biophys Acta.2011;1813(11):1938-1945．
[9] Kim KM, Park SJ, Jung SH, et al. miR-182 is a negative regulator of osteoblast proliferation, differentiation, and skeletogenesis through targeting FoxO1.J Bone Miner Res. 2012;27 (8):1669-1679.
[10] Kousteni S.FoxOs: Unifying links between oxidative stress and skeletal homeostasis. Curr Osteoporos Rep.2011;9 (2): 60-66.
[11] Bartell SM, Kim HN, Ambrogini E, et al.FoxO proteins restrain osteoclastogenesis and bone resorption by attenuating H2O2 accumulation. Nat Commun. 2014;(30)5:3773.
[12] 文思敏,卜淑敏,任景萍.全身垂直振动对去卵巢骨质疏松大鼠松质骨微结构的影响[J].北京体育大学学报, 2013,(36)4: 58-61.
[13] 卜淑敏,李亚义,张颢,等.去卵巢大鼠骨髓基质细胞beta-catenin蛋白表达变化观察[J].中国骨质疏松杂志,2008,14(8):548-551.
[14] Saville PD,Changes in skeletal mass and fragility with castration in the rat: a model of osteoporosis.J Am Geriatr Soc.1969;17 (2):155-166．
[15] Rodgers JB, Faugere M. Animal models for the study of bone loss after cessation of ovarian function．Bone.1993;14: 89-94.
[16] Lelovas PP, Xanthos TT, Thoma SE, et al. The laboratory rat as an animal model for osteoporosis research. Comp Med. 2008;58(5):424-430．
[17] Komrakova M, Sehmisch S, Tezval M, et al. Identification of a vibration regime favorable for bone healing and muscle in estrogen-deficient rats. Calcif Tissue Int.2013;92(6): 509-520.
[18] van der Jagt OP, van der Linden JC, Waarsing JH, et al. Low-magnitude whole body vibration does not affect bone mass but does affect weight in ovariectomized rats. J Bone Miner Metab.2012;30(1):40-46.
[19] Milanese C, Piscitelli F, Zenti MG, et al. Ten-week whole-body vibration training improves body composition and muscle strength in obese women. Int J Med Sci. 2013; 10(3):307-311.
[20] Manolagas SC. From estrogen-centric to aging and oxidative stress: a revised perspective of the pathogenesis of osteoporosis. Endocr Rev.2010;31 (3): 266-300.