Chinese Journal of Tissue Engineering Research ›› 2017, Vol. 21 ›› Issue (8): 1236-1240.doi: 10.3969/j.issn.2095-4344.2017.08.015

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Establishing a rat model of osteoporosis with critical-size calvarial defects

Huang Kui1, Luo Dao-wen1, Wang Lei1, Luo Shi-hong1, Yao Zhi-hao1, Li Yong1, Xiao Jin-gang1, 2   

  1. 1Orofacial Reconstruction and Regeneration Laboratory, 2Department of Oral and Maxillofacial Surgery, the Affiliated Stomatology Hospital of Southwest Medical University, Luzhou 646000, Sichuan Province, China
  • Received:2016-10-20 Online:2017-03-18 Published:2017-04-14
  • Contact: Xiao Jin-gang, M.D., Professor, Chief physician, Master’s supervisor, Orofacial Reconstruction and Regeneration Laboratory, Department of Oral and Maxillofacial Surgery, the Affiliated Stomatology Hospital of Southwest Medical University, Luzhou 646000, Sichuan Province, China
  • About author:Huang Kui, Studying for master’s degree, Orofacial Reconstruction and Regeneration Laboratory, the Affiliated Stomatology Hospital of Southwest Medical University, Luzhou 646000, Sichuan Province, China
  • Supported by:

    the National Natural Science Foundation of China, No. 813671125; the Program of Sichuan Science and Technology Bureau, No. 2014JY0044; the Project of Education Department of Sichuan Province, No. 10-ZB030; the Project of Health Department of Sichuan Province, No. 80170; the Key Project of Luzhou Medical University, No. 201207; Luzhou Municipal People’s Government-the Science and Technology Strategic Cooperation Project of Southwest Medical University, No. 2015LZCYD-S05(2/12)

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Abstract:

BACKGROUND: Osteoporosis with bone defects is becoming a devastating problem. To establish an animal model is the basis of studying this disease, but how to establish the animal model is not yet reported.
OBJECTIVE: To establish a Sprague-Dawley rat model of osteoporosis accompanied with critical-size calvarial defects.
METHODS: Twenty Sprague-Dawley rats were randomly divided into two groups, followed by bilateral ovariectomy, and 8 mm critical-size calvarial defects were established 12 weeks later (experimental group) or followed by removal of the same quality of adipose tissues around bilateral ovaries and 8 mm critical-size calvarial defects were established 12 weeks later (control group). Twelve weeks after modeling, the three-dimensional bone mineral content and bone mineral density of the isolated proximal femur and distal tibial, as well as the level of serum alkaline phosphatase were detected; at the same time, the histological morphology of the skull was observed.
RESULTS AND CONCLUSION: The skull defects in both two groups were not completely healed and the repair outcome was not significant. The new bone formation in the control group was significantly more remarkable than that in the experimental group. Compared with the control group, the three-dimensional bone mineral content and bone mineral density of the proximal femur and distal tibial were significantly decreased, and the level of serum alkaline phosphatase was significantly increased in the experimental group (P < 0.05). Furthermore, in the experimental group, the trabecular bone became smaller and thinner, the bone marrow cavity was obviously increased, the cortical bone appeared to be markedly thinner, and the formation of new bone matrix was significantly decreased. To conclude, the Sprague-Dawley rat model of osteoporosis with critical-size calvarial defects is successfully established.

中国组织工程研究杂志出版内容重点：组织构建；骨细胞；软骨细胞；细胞培养；成纤维细胞；血管内皮细胞；骨质疏松；组织工程

Key words: Osteoporosis, Ovariectomy, Rats, Sprague-Dawely, Tissue Engineering

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