Puerarin effects on the mRNA expression of osteoblast differentiation-related proteins

doi:10.3969/j.issn.2095-4344.2014.42.002

Abstract

Abstract:

BACKGROUND: Experimental studies have showed that puerarin has an obvious protective effect on osteoporosis in ovariectomized and orchiectomized mice. But the influence of puerarin in the molecular level in the process of osteoblast differentiation is seldom reported.
OBJECTIVE: To observe the effect of puerarin on the mRNA expression of alkaline phosphatase, bone sialoprotein, osteopontin and osteocalcin in osteoblasts.
METHODS: The MC3T3-E1 cells from mice cultured in vitro were randomly divided into control group, puerarin group (10^-6 mol/L puerarin) and estradiol group (10^-7 mol/L estradiol) to observe the effects of puerarin on the differentiation of osteoblasts. mRNA expression of alkaline phosphatase, bone sialoprotein, osteopontin and osteocalcin in MC3T3-E1 cells was determined using RT-PCR method.
RESULTS AND CONCLUSION: Puerarin and estradiol both could prolong the expression of alkaline phosphatase that reached the peak at 12 days. Puerarin and estradiol strengthened the mRNA expression of bone sialoprotein at 10 and 12 days, reduced expression of osteopontin at 5 and 12 days, and increased expression of osteocalcin at 10 and 12 days. These results reveal that puerarin can induce the differentiation of cultured osteoblasts by influencing osteoblast differentiation-related protein mRNA expressions, which may be one of the important molecular mechanisms of puerarin for prevention of osteoporosis.

中国组织工程研究杂志出版内容重点：组织构建；骨细胞；软骨细胞；细胞培养；成纤维细胞；血管内皮细胞；骨质疏松；组织工程

全文链接：

Key words: osteoblasts, alkaline phosphatase, osteopontin, osteocalcin, osteoporosis

CLC Number:

R318

Yuan Si-yuan, Kong Bei-bei, Sheng Tong, Wang Xin-xiang, Zhang Yun-ling, Liu Xue-mei, Ma Tao, Zheng Hong, Yan Yan, Liu Lian-qi . Puerarin effects on the mRNA expression of osteoblast differentiation-related proteins[J]. Chinese Journal of Tissue Engineering Research, 2014, 18(42): 6732-6736.

Figures/Tables 5

References

[1] 王新祥,张允岭,吴坚,等.葛根对骨质疏松模型小鼠骨密度和骨组织构造的作用[J].中国骨质疏松杂志,2008,14(5):349-354.
[2] 王新祥,张允岭,吴坚,等.葛根对睾丸切除骨质疏松模型小鼠骨密度和骨构造的作用[J].中国组织工程研究与临床康复, 2010, 14(7):1262-1266.
[3] 尹丽红,李艳枫,孟繁琳.葛根的化学成分、药理作用和临床应用[J].黑龙江医药,2010,23(3):371-373.
[4] Liu XJ, Mei ZG, Qian JP,et al. Puerarin partly counteracts the inflammatory response after cerebral ischemia/reperfusion via activating the cholinergic anti-inflammatory pathway. Neural Regene Res.2013;8(34): 3203-3215.
[5] 黄延玲,石凤英.葛根素对去卵巢大鼠骨密度和骨代谢生化指标的影响[J].中国临床康复, 2004,8(12): 2307-2309.
[6] Huh JE, Yang HR, Park DS, et al. Puerariae radix promotes differentiation and mineralization in human osteoblast-like SaOS-2 cells.Ethnopharmacol. 2006; 104(3):345-350.
[7] 周强,付庭斌.葛根素对去卵巢大鼠骨质疏松性骨折愈合的促进作用[J].中国临床康复,2006,10(27):45-47.
[8] 田泉,汤旭磊,白孟海,等.葛根素对去卵巢大鼠骨质疏松和血脂的作用[J].中华老年医学杂志,2006,25(7):543-545.
[9] 黄彤,金邦荃,孙桂菊,等.葛根素对去卵巢大鼠骨密度和骨强度的改善[J].现代预防医学,2010,37(20):3894-3896.
[10] 李海,金花,黄海玲,等.葛根素对去卵巢大鼠和铅中毒大鼠骨组织的影响[J].现代预防医学,2011,38(18):3742-3744.
[11] 中华医学会骨质疏松和骨矿盐疾病分会.原发性骨质疏松症诊治指南[J].中华骨质疏松和骨矿盐疾病杂志,2011,4(11):2-16.
[12] Colon-Emeric C, Nordsletten L, Olson S, et al.Association between timing of zoledronic acid infusion and hip fracture healing.Osteoporos Int.2010;12(9):1669-1673.
[13] Abrahamsen B, Eiken P, Eastell R.Subtrochanteric and diaphyseal femur fractures in patients treated with alendronate:aregister -based national cohort study.J Bone Miner Res.2009;24(6):1095-1102.
[14] Khan AA, Sándor GK, Dore E, et al.Canadian consensus practice guidelines for bisphosphonate associated osteonecrosis of the jaw.J Rheumatol.2008;35(7):1391-1397.
[15] 赵学文.植物性雌激素对妇女疾病治疗中的作用[J].南通大学学报:医学版, 2005,25(3):231-232.
[16] Zhang Y, Chen WF, Lai WP, et al. Soy isoflavones and their bone protective effects.Inflammopharmacology. 2008; 16(5): 213-215.
[17] Atmaca A, Kleerekoper M, Bayraktar M,et al. Soy isoflavones in the management of postmenopausal osteoporosis. Menopause. 2008;15(4 Pt 1):748-757.
[18] Wang Q, Ge X, Tian X, et al. Soy isoflavone: The multipurpose phytochemical (Review).Biomed Rep. 2013; 1(5):697-701.
[19] Lagari VS, Levis S.Phytoestrogens in the prevention of postmenopausal bone loss.Clin Densitom. 2013;16(4): 445-449.
[20] Uehara M.Isoflavone metabolism and bone-sparing effects of daidzein-metabolites.Clin Biochem Nutr. 2013; 52(3):193-201.
[21] Xinxiang Wang, Jian Wu et al.Puerariae radix prevents bone loss in ovariectomized mice. J Bone Miner Metab. 2003;21(5): 268-275.
[22] 郑高利,张信岳,方晓林,等.葛根异黄酮对去卵巢大鼠骨矿密度和骨强度的影响[J].中草药,2001,32 (5):422-425.
[23] 华川,李杨.葛根素对大鼠成骨细胞增殖分化影响的血清药理学实验[J].华北国防医药,2005,17(6):393-395.
[24] 王久亮,郑忠志.葛根素对大鼠成骨细胞增殖和分化的影响[J].中国组织工程研究与临床康复,2007,7(36):7138-7141.
[25] 臧洪敏,陈君长.葛根素对成骨细胞生物学作用的实验研究[J].中国中药杂志,2005,30(24):1947-1949.
[26] 郭辉,刘奕琛.葛根素对大鼠成骨细胞代谢调控的机制的实验研究[J].现代中医药,2008,28(1):48-50.
[27] 王昌,汤旭磊,陈克明,等.葛根素在体外对成骨细胞增殖和分化的影响[J].中国组织工程研究,2012,16(33):6102-6106.
[28] 王建华,潘永梅,詹文红.葛根素对大鼠成骨细胞增殖与分化的影响[J].中药药理与临床,2007,23(2):21-22.
[29] 王海珍,贠云飞,苗志勃.葛根素对大鼠成骨细胞增殖影响的体外实验研究[J].中国医药导报,2013,19(3):77-79.
[30] 王新祥,张允岭,郑宏,等.葛根素定量分析及对卵巢切除小鼠骨密度作用[J]中国科技论文在线,http://www.paper.edu.cn. 201102-82.
[31] Woldarski KH. Properties and origin of osteoblasts. Clin Orthop Relat Res. 1990; (252):276-293.
[32] Fisher IW,Torchia DA,Fohr B,et al.Flexible structure of SIBLINGproteins, bone sialoProtein and osteopontin. Biochem Biollys Res Commun.2001;80:460-465.
[33] Paz J, Wade K, Kiyoshima T, et al. Tissue and bone cell-specific expression of bone sialoprotein is directed by a 9.0 kb promoter in transgenic mice. J ,Matrix Biol. 2005;24(5):341-352.
[34] 盛彤,谢培凤,王新祥,等.神经精神因素对骨代谢的影响[J].中华骨质疏松和骨矿盐疾病杂志,2013,6(2):178-183.