Compression fracture simulation of osteoporotic trabecular
bone in ovariectomized rats

doi:10.3969/j.issn.2095-4344.2622

Abstract

Abstract:

BACKGROUND: At present, related finite element models have been used to simulate the femoral fracture, and the effects of loading rate, loading angle and cancellous bone on the fracture of hip have been discussed. However, the fracture simulation of trabecula is still lack of relevant research.

OBJECTIVE: To simulate the biomechanical process of osteoporotic trabecular compression fracture in ovariectomized rats.

METHODS: The right femur of ovariectomized rats was scanned at the distal end of femur by Micro-CT. The microstructure parameters and three-dimensional model of the region of interest of the rat femur were obtained. After geometric optimization in Geomagic Studio, they were pretreated in HyperMesh 14.0, including volume mesh division, setting material property parameters, boundary conditions, setting load of 1 200 N, acting time of 2 ms, and they were calculated in LS-DYNA software.

RESULTS AND CONCLUSION: (1) The bone trabeculae in the region of interest showed uneven spatial distribution. (2) The bone trabeculae with small volume and small number first presented deformation fracture, and the plate shape and bone trabeculae with large volume finally demonstrated fracture collapse. (3) The change trend of von Mises stress was roughly the same as that of bone trabeculae fracture collapse. (4) The fracture collapse process of bone trabeculae in the region of interest included vertical collapse and horizontal torsion, in which the degree and rate of horizontal torsion were lower than that of vertical collapse, making the size and rate of cross-section torsion angle less than that of coronal plane angle. (5) The increase and peak value of shear stress of failure unit were smaller than Von-mises stress. (6) These results indicate that fracture collapse of bone trabecula is a complex process, including deformation and angle of different planes.

Key words: ovariectomy, osteoporosis, bone trabecula, trabecula fracture and collapse, region of interest, finite element, National Natural Science Foundation of China

CLC Number:

Wu Yuhang, Zheng Liqin, Zhang Biao, Li Fan, Chen Xinmin, Li Musheng, Zheng Yongze, Lin Ziling. Compression fracture simulation of osteoporotic trabecular bone in ovariectomized rats[J]. Chinese Journal of Tissue Engineering Research, 2020, 24(15): 2387-2392.

Figures/Tables 9

References

[1] REEVE J. Role of cortical bone in hip fracture. Bonekey Rep. 2017;6:867.
[2] ARIZA O, GILCHRIST S, WIDMER RP, et al. Comparison of explicit finite element and mechanical simulation of the proximal femur during dynamic drop-tower testing. J Biomech. 2015;48(2):224-232.
[3] 李鹏飞,杜根发,林梓凌,等.基于LS-DYNA模拟老年股骨颈骨折的有限元分析[J].中国组织工程研究, 2016,22(44):6606-6611.
[4] 孙文涛,林梓凌,李凡,等.基于Hypermesh/LS-DYNA仿真股骨干中上1/3骨折的断裂分析[J].广东医学, 2017,38(16): 2481-2483.
[5] KHOR F, CRONIN DS, WATSON B, et al. Importance of asymmetry and anisotropy in predicting cortical bone response and fracture using human body model femur in three-point bending and axial rotation. J Mech Behav Biomed Mater. 2018;87:213-229.
[6] 郑利钦,林梓凌,陈心敏,等.载荷速率对股骨颈骨折裂纹扩展影响的有限元分析[J].中国组织工程研究,2019,23(20):3148-3152.
[7] 郑利钦,林梓凌,何祥鑫,等.有限元法分析不同侧方跌倒角度下股骨颈骨折裂纹扩展的断裂力学特征[J].中国组织工程研究,2019, 23(8):1203-1207.
[8] SAVILLE PD. Changes in skeletal mass and fragility with castration in the rat; a model of osteoporosis. J Am Geriatr Soc. 1969;17(2):155-166.
[9] CORY E, NAZARIAN A, ENTEZARI V, et al. Compressive axial mechanical properties of rat bone as functions of bone volume fraction, apparent density and micro-ct based mineral density. J Biomech. 2010;43(5):953-960.
[10] AN YH, ZHANG J, KANG Q, et al. Mechanical properties of rat epiphyseal cancellous bones studied by indentation testing. J Mater Sci.1997;8(8):493-495.
[11] LI Z, KINDIG MW, KERRIGAN JR, et al. Rib fractures under anterior–posterior dynamic loads: Experimental and finite-element study. J Biomech. 2010;43(2):228-234.
[12] FANTNER GE, BIRKEDAL H, KINDT JH, et al. Influence of the degradation of the organic matrix on the microscopic fracture behavior of trabecular bone. Bone. 2004;35(5): 1013-1022.
[13] XIE P, TANG Z, QING F, et al. Bone mineral density, microarchitectural and mechanical alterations of osteoporotic rat bone under long-term whole-body vibration therapy. J Mech Behav Biomed Mater. 2016;53:341-349.
[14] 葸慧荣,王媛媛,杨芳芳,等. 仙灵骨葆胶囊对去势大鼠骨代谢的影响[J]. 中国中药杂志, 2018,43(13):2751-2757.
[15] 王振昊,林涨源. 葛根素联合锌治疗去势大鼠骨质疏松症的实验研究[J]. 中华全科医学, 2019,17(9):1450-1453.
[16] CESAR R, BLANCO JO, CASTILLERO JB, et al. Biomechanical trabecular bone behavior of calcaneus samples using finite element analysis and experimental tests. IFMBE Proceed. 2015;49:203-207.
[17] SANDINO C, MCERLAIN DD, SCHIPILOW J, et al. Mechanical stimuli of trabecular bone in osteoporosis: A numerical simulation by finite element analysis of microarchitecture. J Mech Behav Biomed Mater. 2017; 66:19-27.
[18] TASSANI S, PANI M, NOAILLY J, et al. Trabecular fracture zone might not be the higher strain region of the trabecular framework. Front Mater. 2018;5:1-9.
[19] MÜLLER R, GERBER SC, HAYES WC. Micro-compression: a novel technique for the nondestructive assessment of local bone failure. Technol Health Care. 1998;6(5):433-444.
[20] THOMAS CDL, MAYHEW PM, POWER J, et al. Femoral neck trabecular bone: loss with aging and role in preventing fracture. J Bone Miner Res. 2009;24(11):1808-1818.
[21] TASSANI S, ÖHMAN C, BARUFFALDI F, et al. Volume to density relation in adult human bone tissue. J Biomech. 2011;44(1):103-108.
[22] LIEBSCHNER MA, KELLER TS. Hydraulic strengthening affects the stiffness and strength of cortical bone. Ann Biomed Eng. 2005;33(1):26-38.
[23] ENNS-BRAY WS, FERGUSON SJ, HELGASON B. Strain rate dependency of bovine trabecular bone under impact loading at sideways fall velocity. J Biomech. 2018;75:46-52.
[24] 王志鹏,宋敏,赵文韬,等.骨质疏松症药物治疗的有限元分析研究进展[J].中国骨质疏松杂志,2018,24(7):971-974.