Changes of bone mineral density, bone metabolism and biomechanics in rat models of ovariectomy and dexamethasone-induced osteoporosis treated by Eucommia ulmoides combined with vibration training

doi:10.3969/j.issn.2095-4344.1458

Abstract

Abstract:

BACKGROUND: Long-term use of glucocorticoids in women after menopause is higher than other stages, and the incidence of osteoporosis is significantly increased. The use of exercise combined with traditional Chinese medicine to prevent and treat osteoporosis has gradually been adopted in the clinic.
OBJECTIVE: To investigate the effects of Eucommia ulmoides extract combined with whole body vibration training on bone mineral density, bone metabolism and bone biomechanical properties in ovariectomized dexamethasone-induced osteoporotic rats.
METHODS: Eighty female Sprague-Dawley rats were purchased and were randomly divided into model and sham groups. The rats in the model group underwent oophorectomy and dexamethasone. The rats in the sham group received sham operation and placebo intake. The modeling process was completed for 6 weeks. After the modeling, the osteoporotic rats were randomly divided into model control group, whole body vibration group, Eucommia ingestion group, and Eucommia inhalation + whole body vibration group, and 12 weeks of intervention was conducted. Within 24 hours after the end of intervention, all rats were placed in a metabolic cage for 24-hour urine collection. The next day, blood sample was collected, and all rats were sacrificed after analyzing bone metabolism. The left femur was scanned and bone biomechanical properties were detected.
RESULTS AND CONCLUSION: (1) Before intervention, the rat body mass in the sham group was significantly lower than that in the other groups (P < 0.05). After intervention, the body mass in the whole body vibration, Eucommia ingestion and Eucommia inhalation + whole body vibration groups was significantly lower than that in the model control group (P < 0.05). The body mass in the model control, whole body vibration, Eucommia ingestion and Eucommia inhalation + whole body vibration groups was significantly higher than that in the sham group (P < 0.05). (2) The femoral bone mineral density and serum estradiol level in the model control group were significantly lower than those in the sham, whole body vibration, Eucommia ingestion and Eucommia inhalation + whole body vibration groups (P < 0.05). (3) Serum osteocalcin, alkaline phosphatase concentration, urine deoxypyridoline, Ca2+, and P3+ in the model control group were significantly higher than those in the sham, whole body vibration, Eucommia ingestion and Eucommia inhalation + whole body vibration groups (P < 0.05). (4) The maximum stress and maximum load in model control group were significantly lower than those in the sham, whole body vibration, Eucommia ingestion and Eucommia inhalation + whole body vibration groups (P < 0.05). (5) The elastic modulus in the model control group was significantly higher than those in the sham, whole body vibration, Eucommia ingestion and Eucommia inhalation + whole body vibration groups (P < 0.05). (6) To conclude, ingestion of Eucommia ulmoides extract, whole body vibration training, ingestion of Eucommia ulmoides extract combined with whole body vibration training can significantly improve the bone mineral density of ovariectomized rats combined with dexamethasone- induced osteoporosis, enhance the biomechanical properties of the femur, and improve bone metabolism indicators.

Key words: osteoporosis, glucocorticoids, Eucommia ulmoides, whole body vibration training, bone mineral density, biomechanics, osteocalcin, alkaline phosphatase

CLC Number:

Wang Xiaoxiao1, Luo Zhizhu2, Liu Haowei2. Changes of bone mineral density, bone metabolism and biomechanics in rat models of ovariectomy and dexamethasone-induced osteoporosis treated by Eucommia ulmoides combined with vibration training [J]. Chinese Journal of Tissue Engineering Research, 2019, 23(31): 4998-5004.

Figures/Tables 3

表2显示：①模型对照组大鼠的股骨骨密度、血清雌二醇水平低于假手术组、全身振动组、杜仲摄入组杜仲摄入+全身振动组大鼠，且差异有显著性意义(P < 0.05)；②假手术组大鼠股骨骨密度、血清雌二醇水平高于杜仲摄入组、全身振动组、杜仲摄入+全身振动组，但差异无显著性意义(P > 0.05)。 2.4 大鼠骨代谢指标检测结果见表3。表3显示：①血清骨钙、碱性磷酸酶水平、尿液脱氧吡啶/肌酐、钙/肌酐、磷/肌酐水平方面，模型对照组大鼠高于假手术组、全身振动组、杜仲摄入组和杜仲摄入+全身振动组，差异有显著性意义(P < 0.05)；假手术组大鼠低于杜仲摄入组、全身振动组、杜仲摄入+全身振动组，但差异无显著性意义(P > 0.05)；②大鼠血钙和血磷方面，模型对照组大鼠高于假手术组、全身振动组、杜仲摄入组和杜仲摄入+全身振动组，但差异无显著性意义(P > 0.05)；假手术组、全身振动组、杜仲摄入组和杜仲摄入+全身振动组组间差异无显著性意义(P > 0.05)。 2.5 各组大鼠股骨骨生物力学指标结果见表4。表4显示：①最大应力、最大载荷方面，模型对照组大鼠低于假手术组、全身振动组、杜仲摄入组和杜仲摄入+全身振动组，差异有显著性意义(P < 0.05)；假手术组大鼠高于杜仲摄入组、全身振动组，差异有显著性意义(P < 0.05)；假手术组大鼠高于杜仲摄入+全身振动组，但差异无显著性意义(P > 0.05)；②断裂载荷方面，模型对照组大鼠高于假手术组、全身振动组、杜仲摄入组和杜仲摄入+全身振动组，差异有显著性意义(P < 0.05)；假手术组、全身振动组、杜仲摄入组和杜仲摄入+全身振动组组间差异无显著性意义(P > 0.05)；③弹性模量方面，模型对照组大鼠高于假手术组、全身振动组、杜仲摄入组和杜仲摄入+全身振动组，差异有显著性意义(P < 0.05)；假手术组大鼠高于杜仲摄入组，差异有显著性意义(P < 0.05)；假手术组大鼠高于全身振动组、杜仲摄入+全身振动组，但差异无显著性意义(P > 0.05)。"

References

[1]Cotts KG, Cifu AS. Treatment of osteoporosis. JAMA. 2018; 319 (10) :1040.

[2]Cano A, Chedraui P, Goulis DG,et al. Calcium in the prevention of postmenopausal osteoporosis: EMAS clinical guide. Maturitas. 2018;107(3):7-12.

[3]Paci M,Burks S,Wang MY. Consensus guidelines for the treatment of osteoporosis. Neurosurgery. 2018;82(1):N6.

[4]Shams-White MM,Chung M,Fu Z, et al. Animal versus plant protein and adult bone health: A systematic review and meta-analysis from the National Osteoporosis Foundation. Plos One. 2018;13(2):e0192459.

[5]Chen YM,Lee HC,Chen MT,et al. Dehydroepiandrosterone supplementation combined with Weight-Loading Whole-Body Vibration Training (WWBV) affects exercise performance and muscle glycogen storage in middle-aged C57BL/6 mice. Int J Med Sci. 2018; 15(6):564-573.

[6]Yoon J,Kanamori A,Fujii K,et al. Evaluation of maslinic acid with whole-body vibration training in elderly women with knee osteoarthritis. Plos One. 2018;13(3):e0194572.

[7]Siebert MJ,Böselt T,Greulich T,et al. Effects of a 6 week whole-body vibration training (WBVT) in stable COPD patients: a randomized clinical trial. Pneumologie. 2017; 71(S1) :PA2216.

[8]Lynch JH. ACSM's Clinician profile. Curr Sports Med Rep. 2018; 17(5):141

[9]卢其腾,农小连,吴家昌,等. 去势联合地塞米松建立大鼠骨质疏松模型的研究[J].中国骨质疏松杂志,2015,21(1):34-36.

[10]Cheng ML,Gupta V. Premenopausal osteoporosis. Endocrinol Metabol Clin North. 2013;17(2):240-244.

[11]Kenanidis E,Potoupnis ME,Kakoulidis P,et al.Management of glucocorticoid-induced osteoporosis:clinical data in relation to disease demographics,bone mineral density and fracture risk. Expert Opin Drug Saf. 2015;14(7):1035-1053.

[12]Seno T,Yamanoto A,Kukida Y,et al. Once-weekly teriparatide improves glucocorticoid - induced osteoporosis in patients with inadequate response to bisphosphonates. Springerplus. 2016;5(1):1056-1063.

[13]Zhu L,Chen J,Zhang J,et al. Parathyroid hormone(PTH) induces autophagy to protect osteocyte cell survival from dexa-Nmethasone damage. Med Sci Monit. 2017;23(21): 4034-4040.

[14]Tolba MF,El-Serafi AT,Omar HA. Caffeic acid phenethyl ester protects against glucocorticoid- induced osteopo-rosis in vivo:impact on oxidative stress and RANKL/OPG signals. Toxicol Appl Pharmacol. 2017;324(29):26-35.

[15]李三华,陈全利,杨加强. 杜仲总黄酮对去卵巢大鼠骨组织代谢的影响[J]. 中国老年学杂志,2018,38(7):3198-3200

[16]骆瑶,陈兰英,官紫祎,等. 杜仲提取物对去卵巢骨质疏松大鼠骨代谢?骨密度及骨微结构的影响[J].中药材, 2016,39(11): 2624-2628.

[17]Corina M,Vulpoi C,Branisteanu D. Relationship between bone mineral density, weight, and estrogen levels in pre and postmenopausal women. Rev Med Chir Soc Med Nat Iasi. 2011; 116(4): 946-950.

[18]Davison KS,Kendler DL,Ammann P,et al. Assessing fracturerisk and effects of osteoporosis drugs: bone mineral densityand beyond. Am J Med. 2009;122(11): 992-997.

[19]Huang RP,Rubin CT,Mcleod KJ. Changes in postural muscle dynamics as a function of age. J Gerontol A Biol Sci Med Sci. 1999;54(8): 352-357.

[20]巴洪冰,程亮.全身振动训练对老年女性骨密度的影响[J].中国骨质疏松杂志,2016,22(3):324-342.

[21]朱再胜,章振林. 不同模式骨折风险评估工具在绝经后女性的应用和评估[J].中国全科医学,2014, 17(16): 1851-1854.

[22]祁珊珊,王永吉,庞田田,等. 去卵巢大鼠骨?生殖系统组织病理学与形态计量学观察[J].中国骨质疏松杂志,2015, 21(7): 774-779.