Hyperbaric oxygen for limb ischemia-reperfusion with no reflow phenomenon: expression of vascular endothelial growth factor and intracellular adhesion molecule 1

doi:10.3969/j.issn.2095-4344.2014.42.015

Abstract

Abstract:

BACKGROUND: Clinical limb ischemia-reperfusion with no reflow phenomenon after vascular injury repair seriously affects the prognosis of patients. Therapeutic effect of hyperbaric oxygen in myocardial ischemia reperfusion is exactly confirmed, but hyperbaric oxygen is rarely reported in the treatment of limb ischemia-reperfusion.
OBJECTIVE: To detect the expression of vascular endothelial growth factor and intracellular adhesion molecule 1 and to investigate the effect of hyperbaric oxygen on the prognosis of limb ischemia-reperfusion with no reflow phenomenon.
METHODS: By clinical screening, cases of main arterial injury of the limbs were selected and subjected to vascular repair for restoring limb blood supply. After surgery, all the cases were randomly divided into two groups (Combined treatment group and surgical group), 16 cases in each group. Combined treatment group was treated with hyperbaric oxygen combined with clinical anticoagulant, antiplatelet treatment; surgical group treated with only postoperative clinical treatment. Another 16 adult healthy volunteers were selected to receive hyperbaric oxygen as hyperbaric oxygen group. Expressions of vascular endothelial growth factor and intracellular adhesion molecule 1 were detected using enzyme-linked immunosorbent assay at 8, 72 hours and 7 days after surgery.
RESULTS AND CONCLUSION: In the combined treatment and surgical groups, patients exhibited higher expressions of vascular endothelial growth factor and intracellular adhesion molecule 1 than those in the hyperbaric oxygen group (P < 0.01). After 72 hours, the expression of vascular endothelial growth factor was significantly higher in the combined treatment group than the surgical group (P < 0.01). At 8 and 72 hours, the expression of intracellular adhesion molecule 1 was lower in the combined treatment group than the surgical group (P < 0.05). These findings suggest that hyperbaric oxygen can induce high expression of vascular endothelial growth factor and inhibit intracellular adhesion molecule 1, which is crucial for improving growth of blood capillary, formation of endothelial cells, reducing no reflow phenomenon.

中国组织工程研究杂志出版内容重点：组织构建；骨细胞；软骨细胞；细胞培养；成纤维细胞；血管内皮细胞；骨质疏松；组织工程

全文链接：

Key words: hyperbaric oxygenation, reperfusion injury, vascular endothelial growth factors, cell adhesion molecules

CLC Number:

R318

Fu Zhao-jun, Xiang Min-hua, Wei Jian, Zhang Chen. Hyperbaric oxygen for limb ischemia-reperfusion with no reflow phenomenon: expression of vascular endothelial growth factor and intracellular adhesion molecule 1[J]. Chinese Journal of Tissue Engineering Research, 2014, 18(42): 6800-6804.

Figures/Tables 1

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