Effects of androgen on the expression of Bcl-2, Bax and Cyt-C in brain tissue of adult rat models of middle cerebral artery occlusion

doi:10.3969/j.issn.2095-4344.1383

Abstract

Abstract:

BACKGROUND: Different extents of sex hormone imbalance occur at acute stage of cerebral infarction in males and females. Decreased testosterone and increased estradiol appear in males, which return to be normal with condition improved.
OBJECTIVE: To explore the effects of androgen on the expression levels of Bcl-2, Bax and Cyt-C in brain tissue after cerebral ischemia/reperfusion injury.
METHODS: One hundred and twenty healthy adult male Sprague-Dawley rats were provided by Laboratory Animal Center of Southwest Medical University, and the study was approved by the Experimental Animal Ethics Committee of Southwest Medical University, approval No. 20170821026. The rats were randomly divided into sham, control, low- and high-dose groups. The rat model of middle cerebral artery occlusion was prepared by advanced Longa method. The sham group received no plug wire. Both groups were divided into five subgroups according to the reperfusion time (6, 12, 24, 48 and 72 hours) at 2 hours after cerebral ischemic (n=5/group). Five rats were selected from each group for detecting the infarct volume at 24 hours. The in situ apoptotic cells were detected by TUNEL method. The expression levels of Bcl-2, Bax and Cyt-C were tested by immunohistochemical staining.
RESULTS AND CONCLUSION: (1) The sham group had no infarction, and few in situ apoptotic cells, and Bcl-2, Bax and Cyt-C expressions had no dynamic change. Compared with the control group, the low-dose group had decreased infarct volume percentage, apoptotic cells, and the expression levels of Bax and Cyt-C, and increased Bcl-2 expression (all P < 0.05). The high-dose group was contrary (all P < 0.05). (2) Except for sham group, the expression levels of Bcl-2 and Bax were gradually increased at 6 hours after cerebral ischemia/reperfusion injury, and peaked at 24 hours, followed by decreased. (3) The above data suggest that after cerebral ischemia/reperfusion injury, low-dose androgen may play a neuroprotective role by reducing the number of apoptotic cells and the expression levels of Bax and Cyt-C and increasing the expression of Bcl-2. The high dose has the opposite effect.

中国组织工程研究杂志出版内容重点：组织构建；骨细胞；软骨细胞；细胞培养；成纤维细胞；血管内皮细胞；骨质疏松；组织工程

Key words: testosterone, cerebral ischemia/reperfusion injury, Bcl-2, bax, Cyt-c, apoptosis, male rats

CLC Number:

Long Yanfang1, 2, Wang Xinlei2, Wang Mingpu3, Tang Xingjiang2. Effects of androgen on the expression of Bcl-2, Bax and Cyt-C in brain tissue of adult rat models of middle cerebral artery occlusion[J]. Chinese Journal of Tissue Engineering Research, 2019, 23(27): 4344-4349.

Figures/Tables 5

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