The interaction between arginase II and microRNA-17 in human pulmonary artery smooth muscle cells

doi:10.3969/j.issn.2095-4344.2014.42.006

Abstract

Abstract:

BACKGROUND: microRNA-17 is confirmed to play an important role in the development of pulmonary hypertension. Some research has shown that hypoxia-induced proliferation in human pulmonary artery smooth muscle cell depends on the induction of arginase II. There is no report about whether there is some interaction between microRNA-17 and arginase II in human pulmonary artery smooth muscle cells.
OBJECTIVE: To investigate the possible interactions between microRNA-17 and arginase II in hypoxic human pulmonary artery smooth muscle cells.
METHODS: Passage 4 human pulmonary artery smooth muscle cells were cultured in 21% O₂ and 5% CO₂ (normoxia) or 1% O₂ and 5% CO₂ (hypoxia), and then transfected with mimic or inhibitor of microRNA-17 or arginase II-small interfering RNA. RNA, microRNA and protein were isolated separately. Expression of microRNA-17 and arginase II was detected with real-time quantitative PCR and western blot assay.
RESULTS AND CONCLUSION: The level of microRNA-17 was significantly increased in cultured human pulmonary artery smooth muscle cells exposed to 1% O₂ hypoxia, as was arginase II mRNA and protein expression. Furthermore, inhibition of microRNA-17 expression decreased the mRNA and protein levels of arginase II in the human pulmonary artery smooth muscle cells under hypoxia. Conversely, over-expression of microRNA-17 increased the mRNA and protein levels of arginase II in the human pulmonary artery smooth muscle cells under normoxia and hypoxia. Knockdown of arginase II by siRNA abolished the hypoxia-induced up-regulation of microRNA-17 expression. These findings indicate that arginase II is a target gene of microRNA-17 and can regulate the expression of microRNA-17 in human pulmonary artery smooth muscle cells.

中国组织工程研究杂志出版内容重点：组织构建；骨细胞；软骨细胞；细胞培养；成纤维细胞；血管内皮细胞；骨质疏松；组织工程

全文链接：

Key words: hypertension, pulmonary, myocytes, smooth muscle, microRNAs, arginase

CLC Number:

R318

Jin You-peng, Pang Ting-ting, Wang Wei, Wang Yu-lin. The interaction between arginase II and microRNA-17 in human pulmonary artery smooth muscle cells[J]. Chinese Journal of Tissue Engineering Research, 2014, 18(42): 6752-6757.

Figures/Tables 4

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