miR-93 effects on the proliferation, apoptosis and invasion of tumor stem cells in thyroid papillary carcinoma

doi:10.3969/j.issn.2095-4344.0957

Abstract

Abstract:

BACKGROUND: Previous studies have found that miR-93 acts as a protective factor rather than a virulence factor involved in the occurrence and development of thyroid adenomas and stress insulin resistance.
OBJECTIVE: To study the effect of miR-93 on the proliferation, apoptosis and invasion of tumor stem cells in thyroid papillary carcinoma.
METHODS: Flow cytometry was used to sort CD44⁺CD24^- cells (cancer stem cells) and CD44⁺CD24^- cells in human thyroid cancer cells TPC-1. The expression levels of miR-93 gene were detected in these two kinds of cells. CD44⁺CD24^-cancer stem cells were transfected with miR-93 mimics and miRNA mimics. Then, cell proliferation, apoptosis and invasion were detected through cell counting kit-8, flow cytometry, Transwell assay, respectively. Western blot assay was also used to measure expression of Ki67, Bax, Bcl-2, matrix metalloproteinases 2 and 9.
RESULTS AND CONCLUSION: The expression of miR-93 in CD44⁺CD24^- cells was significantly lower than that in non-CD44⁺CD24^- cells (P < 0.05). CD44⁺CD24^- tumor stem cells transfected with miR-93 mimics exhibited lower proliferation (P < 0.05) and lower Ki67 expression than those transfected with miRNA mimics (P < 0.05). Compared with the miRNA mimics group, higher apoptotic rate, higher Bax expression and lower Bcl-2 expression were detected in the miR-93 mimics group (P < 0.05). Cell invasion ability and expression levels of matrix metalloproteinases 2 and 9 were also lower in the CD44⁺CD24^- cancer stem cells transfected with miR-93 mimics than those transfected with miRNA mimics (P < 0.05). Our experimental findings suggest that miR-93 can inhibit the proliferation and invasion of tumor stem cells in thyroid papillary carcinoma and promote cell apoptosis.

中国组织工程研究杂志出版内容重点：干细胞；骨髓干细胞；造血干细胞；脂肪干细胞；肿瘤干细胞；胚胎干细胞；脐带脐血干细胞；干细胞诱导；干细胞分化；组织工程

Key words: Thyroid Neoplasms, Neoplastic Stem Cells, MicroRNAs, Transfection, Cell Proliferation, Apoptosis, Tissue Engineering

CLC Number:

R394.2

He Xin-zhi, Lu Guan-ming, Pu Jian, Wei Zhong-heng, Ma Yan-fei, Chen Yong-cheng, Qin Qiang, Huang Qian-fang, Luo Zhi-zhai. miR-93 effects on the proliferation, apoptosis and invasion of tumor stem cells in thyroid papillary carcinoma[J]. Chinese Journal of Tissue Engineering Research, 2018, 22(25): 3987-3992.

Figures/Tables 1

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