Gene chipset analysis in rats with non-alcoholic fatty liver disease intervened by polyene phosphatidylcholine

doi:10.3969/j.issn.2095-4344.0619

Abstract

Abstract:

BACKGROUND: With the development of information technology and integration with the biomedical field, a large number of big data related to genomics and transcriptomics, have been generated, and these data are analyzed through a combination of bioinformatics and computer science. Further the interrelationships between multiple genes are explored. The targets of polyene phosphatidylcholine protecting liver, improving lipid metabolism and intervening nonalcoholic fatty liver and underlying mechanisms remain unclear.
OBJECTIVE: To predict and investigate the regulatory mechanism of polyene phosphatidylcholine improving fat metabolism of hepatocyte based on screening the difference between mRNA (Clariom™ S Assay) and miRNA expression.
METHODS: Serum of Sprague-Dawley rats receiving intragastric administration of polyene phosphatidylcholine was extracted. BRL rat hepatocytes were cultured in the medium containing 50% of fetal bovine serum to establish the model of nonalcoholic fatty hepatocytes. Then polyene phosphatidylcholine serum was used as experimental group and negative control serum was used as control group, respectively. Total RNAs were extracted and differently expressed miRNAs were detected by Clariom S expression profile chip for expression level of mRNA and miRNA. Bioinformatics method was used to predict target genes of differential miRNAs regulation. Enrichment of functions and signaling pathways of the target genes was conducted by fat metabolism related genes.
RESULTS AND CONCLUSION: In screening and predicting, 5 629 target genes were involved in 13 kinds of biological processes, 14 kinds of cellular components, and 11 kinds of molecular functions. Metabolic pathways, purine metabolism and steroid biosynthesis pathway were enriched. rno-miR-21-5p, rno-miR-370-3p, rno-miR-542-3p were the center of the molecular network. According to IPA database, 56 genes with fat metabolism effective interaction were screened out. These results show rno-miR-21-5p, rno-miR-370-3p, rno-miR-542-3p and Metabolic pathways, purine metabolism and steroid biosynthesis pathway may play a role in the differentiation process of non-alcoholic fatty liver induced by phosphatidylcholine.

中国组织工程研究杂志出版内容重点：组织构建；骨细胞；软骨细胞；细胞培养；成纤维细胞；血管内皮细胞；骨质疏松；组织工程

Key words: Fatty Liver, Computational Biology, MicroRNAs, Tissue Engineering

CLC Number:

R318

Liu Rui1, He Jia2, Liu Xiufang2, Feng Xiaoqiu1, Gu Zhanxin2, Kang Shanping1. Gene chipset analysis in rats with non-alcoholic fatty liver disease intervened by polyene phosphatidylcholine[J]. Chinese Journal of Tissue Engineering Research, 2018, 22(36): 5833-5839.

Figures/Tables 2

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