Chinese Journal of Tissue Engineering Research ›› 2019, Vol. 23 ›› Issue (7): 1103-1108.doi: 10.3969/j.issn.2095-4344.1059

Previous Articles     Next Articles

Genotype identification and model application of apolipoprotein E knockout mice

Zhang Wenjiang1,2, Yi Jian3, Jia Ping1, Chen Bowei1, Wang Yong2, Liu Baiyan1   

  1.  (1Hunan University of Chinese Medicine, Changsha 410208, Hunan Province, China; 2Yiyang Medical College, Yiyang 413000, Hunan Province, China; 3the First Affiliated Hospital of Hunan University of Chinese Medicine, Changsha 410007, Hunan Province, China)
  • Received:2018-10-07 Online:2019-03-08 Published:2019-03-08
  • Contact: Liu Baiyan, MD, Professor, Doctoral supervisor, Hunan University of Chinese Medicine, Changsha 410208, Hunan Province, China
  • About author:Zhang Wenjiang, Doctoral candidate, Hunan University of Chinese Medicine, Changsha 410208, Hunan Province, China; Yiyang Medical College, Yiyang 413000, Hunan Province, China
  • Supported by:

    the Science and Technology Project of Hunan Province, No. 2018JJ2412 (to ZWJ); the Science Research Program of Hunan University of Chinese Medicine, No. 201870 (to ZWJ); the Project of Education Department of Hunan Province, No. 17B271 (to ZWJ)

PDF

204

Abstract:

BACKGROUND: Apolipoprotein E knockout mice are an ideal model of atherosclerosis, which has been reported in the other diseases.
OBJECTIVE: To systematically summarize the application of apolipoprotein E knockout mice in disease models.
METHODS: Six male and six female APOE-/- mice introduced from the Biomedical Research Center of Nanjing University were bred in each cage at 1:1 in the SPF laboratory. The genomic DNA was extracted from mouse tail tissue by the method of boiling lysis, and the genotypes were detected by PCR. The APOE-/- mice were fed with normal diet for 8 weeks, and then fed with a Western diet (provided by Beijing Huafukang Bioscience Co., Ltd., No. H10141, 21% of fat, 0.15% of cholesterol) to 25 week old. At the same time, 10 8-week-old C57/6J mice were fed with a normal diet to 25 weeks old.
RESULTS AND CONCLUSION: Genetic identification: agarose gel electrophoresis results indicated that the size of molecular weight of the PCR products from APOE-/- gene knockout mice was consistent with the expected target gene fragment and of different genotypes successfully. Changes of related indicators: compared with the wild type mice, the body mass, liver weight, spleen weight, abdominal fat weight, liver index, and serum levels of total cholesterol, triacylglycerol, low density lipoprotein cholesterol, high density lipoprotein cholesterol, alanine transaminase, and aspartate transaminase in the APOE-/- mice were significantly increased (P < 0.05). The histological changes: APOE-/- mice showed obvious plaques in the aortic arch, aortic valve and common carotid artery by hematoxylin-eosin staining, but no plaques in the wild type mice. The liver of APOE-/- mice had vacuoles of varying sizes, and the nucleus was pushed aside. The nucleus of wild mice liver was in the middle, the cytoplasm was red, and there was no vacuole phenomenon. These results suggest that PCR can fast and reliably identify the genotypes of apolipoprotein E knockout mice using genomic DNA extracted by boiling lysis. APOE-/- mice are ideal models for studying atherosclerosis, fatty liver, hyperlipidemia, hypercholesterolemia, obesity and many other diseases.

中国组织工程研究杂志出版内容重点：组织构建；骨细胞；软骨细胞；细胞培养；成纤维细胞；血管内皮细胞；骨质疏松；组织工程

Key words: Tissue Engineering, Atherosclerosis, Fatty Liver, Hyperlipidemias

CLC Number: