Synergistic resistance of moderate-intensity endurance exercise and allicin to rat models of fatty liver induced by high-fat diet

doi:10.3969/j.issn.2095-4344.1338

Abstract

Abstract:

BACKGROUND: The incidence of non-alcoholic fatty liver induced by high-fat diet and sports deficiency is on a rise. Endurance exercise and allicin can improve blood lipid metabolism, but the protective effect of endurance exercise combined with allicin from fatty liver formation in rats induced by high-fat diet is rarely reported.
OBJECTIVE: To investigate the effect of moderate-intensity endurance exercise combined with allicin on the formation of fatty liver in rats fed with high-fat diet.
METHODS: The study was approved by the Experimental Animal Ethics Committee of Guangxi Normal University. Sixty adult male Sprague-Dawley rats were randomly divided into normal diet control group, high-fat diet group, high-fat diet placebo (distilled water via gavage) group, high-fat diet exercise group, high-fat diet allicin group and high-fat diet exercise combined with allicin (allicin via gavage) group. The endurance exercise was performed for 30 minutes once daily, 6 days/week. The expression and activity of heme oxygenase-1 protein and malondialdehyde content in rat liver were detected. The concentration changes of alanine aminotransferase, glutamic oxalate aminotransferase, triglyceride, free fatty acid and total cholesterol in blood were measured. The morphological changes of liver tissue were observed and the activity integral of non-alcoholic fatty liver was calculated.
RESUITS AND CONCLUSION: (1) High-fat diet significantly increased blood triglyceride, free fatty acid, total cholesterol, alanine aminotransferase and glutamic oxalate aminotransferase (P < 0.05), decreased liver heme oxygenase-1 activity and increased malondialdehyde content (P < 0.05), and increased liver steatosis and non-alcoholic fatty liver activity integral (P < 0.05). (2) Compared with the high-fat diet group, the expression level and activity of heme oxygenase-1 protein in the lipid liver of the high-fat diet exercise, high-fat diet exercise, high-fat diet exercise, high-fat diet allicin, and high-fat diet exercise combined with allicin groups were significantly increased (P < 0.05). (3) Compared with the high-fat diet group, in the high-fat diet exercise, high-fat diet combined with allicin group, the blood triglycerides, free fatty acid, total cholesterol, concentration of alanine aminotransferase and glutamic oxalate aminotransferase were decreased significantly (P < 0.05); the content of malondialdehyde in liver was decreased significantly (P < 0.05); the fatty degeneration of liver tissue was improved significantly; and the activity score of non-alcoholic fatty liver decreased significantly (P < 0.05). The above changes were most obvious in the high-fat diet and exercise combined with allicin group. (4) In summary, moderate-intensity endurance exercise and allicin have synergistic resistance to fatty liver formation in rats fed with high-fat diet, which may be related to the synergistic control of lipid peroxidation in liver, triglyceride, free fatty acid and total cholesterol concentration in blood.

Key words: endurance exercise, allicin, non-alcoholic fatty liver, blood lipid, heme oxygenase-1, lipid peroxidation

CLC Number:

R496，R318

Yang Zhongming1, Jiang Manyi2, Xu Simao3 . Synergistic resistance of moderate-intensity endurance exercise and allicin to rat models of fatty liver induced by high-fat diet[J]. Chinese Journal of Tissue Engineering Research, 2019, 23(31): 5030-5035.

Figures/Tables 6

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