Salvia miltiorrhiza extract protects H9C2 cardiomyocytes by up-regulation of heme oxygenase-1

doi:10.3969/j.issn.2095-4344.0823

Abstract

Abstract:

BACKGROUND: Pathogenesis of myocardial ischemia/reperfusion injury is related to oxidative stress. Previous studies have shown that Salvia miltiorrhiza can improve the apoptosis of cardiomyocytes post acute myocardial infarction.
OBJECTIVE: To investigate the protective effects of heme oxygenase-1 (HO-1) mediated Salvia miltiorrhiza extract on the oxidative stress-induced cell injury in H9C2 cardiomyocytes.
METHODS: H9C2 cardiomyocytes were divided into four groups: control, model, cobalt protoporphyrin and Salvia miltiorrhiza groups. H9C2 cardiomyocytes were treated with 300 μmol/L hydrogen peroxide for 24 hours to establish myocardial cell oxidative damage model. Afterwards, Salvia miltiorrhiza extract or cobalt protoporphyrin was added, while the control group received no treatment.
RESULTS AND CONCLUSION: Compared with the control group, in the model group, the content of creatine kinase and lactate dehydrogenase in cell supernatant, the level of malondialdehyde in myocardial cell lysate, and the expression levels of HO-1 and nuclear transcription factor E2 in myocardial cells were increased significantly, while the level of superoxide dismutase in myocardial cell lysate was significantly decreased. Compared with the model group, the cobalt protoporphyrin and Salvia miltiorrhiza groups revealed a significant decrease in the content of creatine kinase and lactate dehydrogenase in cell supernatant, the level of malondialdehyde in myocardial cell lysate, and the expression levels of HO-1 and nuclear transcription factor E2 in myocardial cells, and a significant increase in the level of superoxide dismutase in myocardial cell lysate. To conclude, Salvia miltiorrhiza extract can protect cardiomyocytes from oxidative stress by HO-1 protein activation.

中国组织工程研究杂志出版内容重点：组织构建；骨细胞；软骨细胞；细胞培养；成纤维细胞；血管内皮细胞；骨质疏松；组织工程

Key words: Salvia miltiorrhiza, Heme Oxygenase-1, Myocytes, Cardiac, Tissue Engineering

CLC Number:

R318

Yu Chun-miao1, Zhao Binjiahui2, Yan Hong-wei3. Salvia miltiorrhiza extract protects H9C2 cardiomyocytes by up-regulation of heme oxygenase-1[J]. Chinese Journal of Tissue Engineering Research, 2018, 22(24): 3888-3892.

Figures/Tables 2

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