Embryo transfer parameters effects on the movement and deposition of embryo in the uterine cavity

doi:10.3969/j.issn.2095-4344.0268

Abstract

Abstract:

BACKGROUND: The rate of embryo implantation is at 20%-30%, suggesting that 70%-80% of the embryos fail in implantation. Except quality of embryo and endometrial receptivity, proper embryo transfer is also an important factor in success.
OBJECTIVE: To investigate the sedimentary position of human embryo into the uterine cavity with different embryo transfer parameters through numerical simulation and in vitro embryo transfer experiment.
METHODS: The combination of 3 parameters including injection time (10, 20, 30 seconds), distance between transfer tube end and bottom of the uterus (5,10, 15 mm), and the uterus angle (-60°, -45°, -30°, -15°, 0°, 15°, 30°, 45°, 60°).
RESULTS AND CONCLUSION: After comparative analysis, 60 μL was selected as the volume of embryo transfer fluid. At different angles of the uterus, the changes in the trajectories of particles and the final sedimentary position were caused by gravity, and the optimal angle was in 15°-30°. Additionally, the optimal injection time was 10 seconds, and the distance between transfer tube end and bottom of the uterus was 10 mm.

中国组织工程研究杂志出版内容重点：组织构建；骨细胞；软骨细胞；细胞培养；成纤维细胞；血管内皮细胞；骨质疏松；组织工程

Key words: Embryo Transfer, Uterus, Tissue Engineering

CLC Number:

R318

Yang Qing1, Tao Shuai1, Niu Zhi-hong2, Mo Jin-qiu1. Embryo transfer parameters effects on the movement and deposition of embryo in the uterine cavity[J]. Chinese Journal of Tissue Engineering Research, 2018, 22(24): 3880-3887.

Figures/Tables 3

References

[1] Eytan O, Elad D, Zaretsky U,et al.A glance into the uterus during in vitro simulation of embryo transfer.Hum Reprod. 2004;19(3):562-569.

[2] Eytan O, Elad D, Jaffa AJ.Evaluation of the embryo transfer protocol by a laboratory model of the uterus.Fertil Steril. 2007; 88(2):485-493.

[3] Eytan O, Zaretsky U, Jaffa AJ,et al.In vitro simulations of embryo transfer in a laboratory model of the uterus.J Biomech. 2007;40(5):1073-1080.

[4] Eytan O, Elad D. Analysis of intra-uterine fluid motion induced by uterine contractions.Bull Math Biol. 1999;61(2): 221-238.

[5] Eytan O, Jaffa AJ, Elad D.Peristaltic flow in a tapered channel: application to embryo transport within the uterine cavity.Med Eng Phys. 2001;23(7):473-482.

[6] Yaniv S, Elad D, Jaffa AJ,et al.Biofluid aspects of embryo transfer.Ann Biomed Eng. 2003;31(10):1255-1262.

[7] Lauko IG, Rinaudo P, Dashev S.A computational parameter study of embryo transfer.Ann Biomed Eng.2007;35(4): 659-671.

[8] Yaniv S, Jaffa AJ, Eytan O,et al.Simulation of embryo transport in a closed uterine cavity model.Eur J Obstet Gynecol Reprod Biol. 2009;144 Suppl 1:S50-60.

[9] Yaniv S, Jaffa AJ, Elad D.Modeling embryo transfer into a closed uterine cavity.J Biomech Eng. 2012;134(11): 111003.

[10] 楚喜英,万利静,宋玉霞,等.冻融胚胎移植位置距宫底距离与妊娠结局的关系[J].广东医学,2014,35(1):86-89.

[11] Cenksoy PO, F?c?c?oglu C，Yesiladali M，et al.The importance of the length of uterine cavity, the position of the tip of the inner catheter and the distance between the fundal endometrial surface and the air bubbles as determinants of the pregnancy rate in IVF cycles.Eur J Obstet Gynecol Reprod Biol. 2014; 172:46-50.

[12] Caanen MR, van der Houwen LE, Schats R,et al.Embryo Transfer with Controlled Injection Speed to Increase Pregnancy Rates: A Randomized Controlled Trial.Gynecol Obstet Invest. 2016;81(5):394-404.

[13] Groeneveld E, de Leeuw B, Vergouw CG, et al. Standardization of catheter load speed during embryo transfer: comparison of manual and pump-regulated embryo transfer. Reprod Biomed Online. 2012;24(2):163-169.

[14] Eytan O, Elad D.Analysis of intra-uterine fluid motion induced by uterine contractions.Bull Math Biol. 1999;61 (2):221-238.

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