18F-FDG effects on maturation and immune function of dendritic cells in vitro

doi:10.3969/j.issn.2095-4344.2014.42.007

Abstract

Abstract:

BACKGROUND: Previous studies have shown that different doses of ionizing radiation may have some impact on maturation and immune function of dendritic cells.
OBJECTIVE: To evaluate the effect of ¹⁸F-FDG with different concentrations on maturation and immune function of dendritic cells from human peripheral blood mononuclear cells in vitro.
METHODS: Human peripheral blood mononuclear cells-derived mature dendritic cells were divided into five groups: PBS, 92.5×10⁴, 185×10⁴, 370×10⁴, 740×10⁴ Bq/mL ¹⁸F-FDG were separately added into each group. Dendritic cells were collected 24 hours later. Apoptosis rate, phenotypic expression (CD1α, CD80, CD83, CD86, HLA-DR), ability of mixed lymphocyte reaction and expression of macrophage inflammatory protein 1α and monocyte chemotactic factor-1 in cell culture supernatant were detected.
RESULTS AND CONCLUSION: Apoptosis rate, phenotypic expression of CD86, ability of mixed lymphocyte reaction and expression of monocyte chemotactic factor-1 were down-regulated after culture in 740×10⁴ Bq/mL ¹⁸F-FDG. However, ¹⁸F-FDG at other concentrations had no influence on maturation and immune function of dendritic cells. This study demonstrates that low-concentration ¹⁸F-FDG has little influence on apoptosis, maturation, antigen presentation, and migratory capacity of dendritic cells, and it may be selected at an appropriate concentration as a label for dendritic cells in vitro.

中国组织工程研究杂志出版内容重点：组织构建；骨细胞；软骨细胞；细胞培养；成纤维细胞；血管内皮细胞；骨质疏松；组织工程

全文链接：

Key words: dendritic cells, chemotactic factors, immunity

CLC Number:

R318

Lei Xiao, Liang Ying-kui, Zhao Wen-rui, Wang Sheng, Guo Feng, Fu Qiang. ¹⁸F-FDG effects on maturation and immune function of dendritic cells in vitro[J]. Chinese Journal of Tissue Engineering Research, 2014, 18(42): 6758-6762.

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References

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¹⁸F-FDG effects on maturation and immune function of dendritic cells in vitro

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