Schisandra extract regulates Th17 cells and regulatory T cell imbalance in dogs undergoing liver transplantation

doi:10.3969/j.issn.2095-4344.0297

Abstract

Abstract:

BACKGROUND: Schisandra extract has various effects, but little is known about the role of Schisandrins extract in immune changes after liver transplantation.
OBJECTIVE: To investigate the effect of Schisandra extract on Th17/regulatory T cells (Treg) imbalance in dogs undergoing liver transplantation.
METHODS: The model dogs undergoing liver transplantation were established, and then divided into control and Schisandra groups (n=10 per group). The control group received glucose and the Schisandra group was given Schisandra extracts postoperatively. The survival rate, liver function, Th17 and Treg cells in peripheral blood, as well as levels of interleukin 17, interleukin 23, interleukin 10 and tumor growth factor β1 in peripheral blood were determined at 1, 3, 6 and 9 days after liver transplantation. The pathological changes of canine liver were compared between groups.
RESULTS AND CONCLUSION: Compared with control group, the survival rate was significantly increased in the Schisandra group (P < 0.05). Compared with the control group, the levels of alanine aminotransferase, aspartate aminotransferase and total bilirubin in the Schisandra group were significantly lower than those in the control group at 3, 6 and 9 days after liver transplantation (P < 0.05). In the control group, the Treg cells were significantly decreased, and Th17 cells were significantly increased (P < 0.05). Compared with the control group, the Th17 and Treg cells in the Schisandra group showed no significant change, but tended to be increased. Compared with the control group, in the Schisandra group, the levels of interleukin 17 and interleukin 23 were significantly decreased, and levels of interleukin 10 and tumor growth factor β1 were significantly increased (P < 0.05). The inflammatory factor infiltration and liver pathology were reduced in the Schisandra group compared with the control group. Our findings imply that Schisandra extracts can significantly improve living conditions, liver function and donor liver’s activity in dogs undergoing liver transplantation, probably by improving the Th17/Treg cell imbalance.

中国组织工程研究杂志出版内容重点：组织构建；骨细胞；软骨细胞；细胞培养；成纤维细胞；血管内皮细胞；骨质疏松；组织工程

Key words: Schisandra, Liver Transplantation, Immunity, Cellular, Tissue Engineering

CLC Number:

R318

Liu Li-ye1, Zhao De-fang2, Gao Fei1, Zhang Tong2, Dong Qin2. Schisandra extract regulates Th17 cells and regulatory T cell imbalance in dogs undergoing liver transplantation[J]. Chinese Journal of Tissue Engineering Research, 2018, 22(20): 3213-3217.

Figures/Tables 3

2.1 手术情况雄性犬共50只，手术成功且存活的犬纳入研究，2个组犬死亡共30只，手术总成功率为40%。 2.2 肝移植后状况对照组肝移植后出现皮毛粗糙、活动少、进食少和精神萎靡，五味子组肝移植后犬的精神较好，活动和进食较多。肝移植后第10天全部安乐处死。对照组和五味子组肝移植后10 d的存活率分别为20%和60%，与对照组比，五味子的生存期明显延长(P < 0.05)，见图1。 2.3 两组肝移植后肝功能的比较两组肝移植后不同时间点谷丙转氨酶、谷草转氨酶及总胆红素水平均有不同程度的升高。肝移植后3，6和9 d五味子组谷丙转氨酶、谷草转氨酶及总胆红素水平显著低于对照组(P < 0.05)，见表1。 2.4 CD4+CD25+Treg+T细胞和Th17(CD4+IL-17+T细胞)占CD4+T细胞比例两组肝移植后1 d的Treg和Th17细胞比例无显著差异(P > 0.05)，肝移植后3，6及9 d，对照组的Treg显著降低(P < 0.05)，Th17显著增高(P < 0.05)，与对照组比，五味子组的Th17增高不明显，Treg没有显著变化(P > 0.05)，但有增高趋势，见表2。 2.5 两组肝移植后外周血外周血白细胞介素17、23、10，转化生长因子β1的比较肝移植后1 d，2组白细胞介素17、23、10，转化生长因子β1无显著差异(P > 0.05)。肝移植后3，6及9 d，与对照组相比，五味子组白细胞介素17和白细胞介素23水平显著降低，白细胞介素10和转化生长因子β1水平显著升高(P < 0.05)，见表3。 2.6 两组肝移植后肝病理变化对照组10 d安乐死犬的肝细胞肿大，淋巴细胞及嗜中性粒细胞浸润，胞浆颗粒变性，偶见肝细胞出血性坏死，对照组肝移植后10 d内死亡犬大量肝细胞浑浊肿胀，嗜酸性坏死，片状坏死，充血。五味子组肝移植后10 d安乐死犬的肝细胞略有肿大，部分淋巴细胞及嗜中性粒细胞浸润，少量胞浆颗粒变性；五味子组肝移植后10 d内死亡犬肝细胞略有肿大，淋巴细胞及嗜中性粒细胞浸润，部分胞浆颗粒变性，充血。见图2。"

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