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    01 October 2014, Volume 18 Issue 41 Previous Issue    Next Issue
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    Construction of endothelial progenitor cells/bone marrow mesenchymal stem cells composite sheets
    Liang Yuan, Sui Ke, Shang Feng-qing, Tang Li, Wang A-xian, Ji Hai-ning, Ding Yin
    2014, 18 (41):  6561-6566.  doi: 10.3969/j.issn.2095-4344.2014.41.001
    Abstract ( 317 )   PDF (845KB) ( 593 )   Save

    BACKGROUND: Many studies have showed that enough blood supply is an essential condition of bone repair and regeneration.

    OBJECTIVE: To construct the endothelial progenitor cells/bone marrow mesenchymal stem cells (EPCs/BMSCs) composite sheet.
    METHODS: After isolation and culture, EPCs and BMSCs were co-cultured directly to form EPCs/BMSCs sheet by cell sheet-inducing medium. After 10 days of induction, the sheet was investigated by gross observation, inverted microscope and hematoxylin-eosin staining. The distribution and communication of EPCs and BMSCs during the process of cell sheet induction were observed after the fluorescence labeling separately. Alkaline phosphatase assay and alizarin red staining were applied to examine the ability of osteogenic differentiation of EPCs/BMSCs sheet.
    RESULTS AND CONCLUSION: EPCs/BMSCs sheet was harvested after 10-day induction. Cell-cell contact between EPCs and BMSCs could be observed during the process of the cell sheet preparation. The harvested sheet was composed of multiple layers of cells and cell-produced extracellular matrix. Alkaline phosphatase assay and alizarin red staining both demonstrated that EPCs/BMSCs sheet had good osteogenic differentiation ability. These results suggested that EPCs/BMSCs sheet can be constructed successfully, and the sheet has strong osteogenic differentiation capability in vitro, providing the foundation for the repair of bone defects.

    中国组织工程研究杂志出版内容重点:干细胞;骨髓干细胞;造血干细胞;脂肪干细胞;肿瘤干细胞;胚胎干细胞;脐带脐血干细胞;干细胞诱导;干细胞分化;组织工程


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    Survival and growth of nano-bioprobe double-labeled rat bone marrow mesenchymal stem cells  
    Cao Ai-hong,Yang Xin, Guo Zi-wei, Hu Wei
    2014, 18 (41):  6567-6572.  doi: 10.3969/j.issn.2095-4344.2014.41.002
    Abstract ( 464 )   PDF (833KB) ( 825 )   Save

    BACKGROUND: Superparamagnetic iron oxide (SPIO) labeling can trace the migration of stem cells in vivo, and the fluorescent DiI dye is suitable for marking and tracing cells because of its less influence on cell viability, proliferation and differentiation.

    OBJECTIVE: To observe the effect and safety of SPIO and fluorescent DiI dye to double label bone marrow mesenchymal stem cells.
    METHODS: The bilateral lower limbs of rats were isolated sterilely. Bone marrow was obtained by rinsing using low-glucose DMEM. Bone marrow mesenchymal stem cells were isolated by the whole bone marrow adherence method and purified by differential attachment method. Purified cells were dual-labeled with SPIO particle and fluorescent DiI dye.
    RESULTS AND CONCLUSION: Bone marrow mesenchymal stem cells could be separated at 8-10 days after primary culture and the subculturing cycle was 3-4 days. Rat bone marrow mesenchymal stem cells could be effectively labeled with SPIO-DiI and the labeling efficiency was almost 100%. Blue irons contained in intracytoplasmatic vesicles could be observed clearly with Prussian blue staining, and the fluorescence microscopy showed red fluorescence at cytoplasm. Survival and apoptosis percentages obtained by MTT analysis were similar among labeled and unlabeled bone marrow mesenchymal stem cells that were both about
    95%.These findings indicate that the rat bone marrow mesenchymal stem cells could be efficiently labeled with SPIO-DiI to construct a nano-bioprobe, without significant changes in morphology, viability and proliferation.

    中国组织工程研究杂志出版内容重点:干细胞;骨髓干细胞;造血干细胞;脂肪干细胞;肿瘤干细胞;胚胎干细胞;脐带脐血干细胞;干细胞诱导;干细胞分化;组织工程


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    Expressions of Bcl-2 and Bax and apoptotic rate of bone marrow mesenchymal stem cells transfected with thymosin beta4 in a hypoxic environment
    Liu De-sheng, Xiao Shi-liang, Wang Bo, Zhou Cheng
    2014, 18 (41):  6573-6577.  doi: 10.3969/j.issn.2095-4344.2014.41.003
    Abstract ( 371 )   PDF (1535KB) ( 729 )   Save

    BACKGROUND: Studies have shown that thymosin β4 can improve the anti-apoptotic ability of a variety of cells, but the reports describing the changes in the anti-apoptotic ability of bone mesenchymal stem cells modified with thymosin β4 gene in the hypoxic environment are rare.

    OBJECTIVE: To observe the change in the apoptotic rate of bone mesenchymal stem cells modified with thymosin β4 gene in the hypoxic environment, and to explore whether thymosin β4 affects the apoptosis of bone marrow mesenchymal stem cells via regulating the expression of Bax and Bcl-2.
    METHODS: Bone marrow mesenchymal stem cells were transfected with the recombinant lentiviral vector over-expressing the thymosin β4 gene, and then we observed the expression of thymosin β4 using western blot assay. Cells were divided into three groups: thymosin β4 transfection group, control virus group, and untreated group. All three groups were placed in a hypoxic environment. The apoptotic rate of bone marrow mesenchymal stem cells was evaluated by the flow cytometry assay. The expression of Bax and Bcl-2 protein was detected by western blot.
    RESULTS AND CONCLUSION: Thymosin β4 gene was expressed successfully in the bone marrow mesenchymal stem cells showed by the western blot. The apoptotic rate of bone marrow mesenchymal stem cells in the hypoxic environment was lower in the thymosin β4 transfection group than the control virus group and untreated group; while there was no difference between the latter two groups. Western blot results showed that the expression of Bcl-2 protein was higher in the thymosin β4 transfection group than the control virus group and untreated group, and the expression of Bax protein was lower in the thymosin β4 transfection group than the control virus group and untreated group. No difference in the expression of Bax and Bcl-2 was found between the control virus group and untreated group. These findings indicate that thymosin β4 overexpression can improve the anti-apoptotic ability of bone mesenchymal stem cells modified in the hypoxic environment, and its possible mechanism is through regulating the expression of Bax and Bcl-2 protein.

    中国组织工程研究杂志出版内容重点:干细胞;骨髓干细胞;造血干细胞;脂肪干细胞;肿瘤干细胞;胚胎干细胞;脐带脐血干细胞;干细胞诱导;干细胞分化;组织工程


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    Three kinds of engineered hepatic tissues constructed using rat bone marrow mesenchymal stem cells
    Yu Jian-xiong, Yuan Jing, Xu Rui-yun
    2014, 18 (41):  6578-6584.  doi: 10.3969/j.issn.2095-4344.2014.41.004
    Abstract ( 314 )   PDF (2654KB) ( 689 )   Save

    BACKGROUND: Engineered hepatic tissue is considered a promising strategy for healing acute liver failure. But, there are series of hindrances in the construction of engineered hepatic tissues, including acquisition of vital hepatocytes, choice of scaffolds and culture system, and nutrition supply.

    OBJECTIVE: To construct three kinds of engineered hepatic tissues in hope to screen the optimal one for transplantation in acute liver failure.
    METHODS: After purification, amplification, bone marrow mesenchymal stem cells were induced to differentiate into hepatocyte-like cells which were co-cultured with acellular amniotic membrane, DACRON PATCH cardiovascular surgical patch, biological surgical patch, respectively to construct three kinds of engineered hepatic tissues. After 3 days of culture, morphological and functional detections were performed.
    RESULTS AND CONCLUSION: Rat bone marrow mesenchymal stem cells with higher purity were successfully harvested by using density gradient centrifugation and adherent methods, and then the cells were differentiated into hepatocyte-like cells. In the three kinds of engineered hepatic tissues, hepatocyte-like cells were found to be combined with the biological surgical patch to the maximum extent, and their combination exhibited stronger ability of urea synthesis and albumin secretion, which provides experimental basis for treatment of acute liver failure.

    中国组织工程研究杂志出版内容重点:干细胞;骨髓干细胞;造血干细胞;脂肪干细胞;肿瘤干细胞;胚胎干细胞;脐带脐血干细胞;干细胞诱导;干细胞分化;组织工程


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    Recombinant CXCR4 gene lentivirus transfection of rat bone marrow mesenchymal stem cells in vitro  
    Cao Zhi-qiang, Wang Yang, Liu Long
    2014, 18 (41):  6585-6590.  doi: 10.3969/j.issn.2095-4344.2014.41.005
    Abstract ( 367 )   PDF (1997KB) ( 1077 )   Save

    BACKGROUND: Chemokine receptor 4 (CXCR4) is the main homing regulating factor for mesenchymal stem cells in vivo. How to improve the self-expression of CXCR4 is crucial for regulating the homing ability of stem cells in vivo targeting organs.

    OBJECTIVE: To construct lentiviral vectors carrying CXCR4 or green fluorescent protein (GFP) gene, and to observe their effects on growth and secrete function of bone marrow mesenchymal stem cells.
    METHODS: Lentiviral vectors were used to over-express CXCR4 in rat bone marrow mesenchymal stem cells. GFP gene was transfected as a control. Transfection efficiency was analyzed using fluorescence microscopy, RT-PCR and western blot assay for detecting the expression of CXCR4 or GFP in cells at both the mRNA and protein levels. Effects of CXCR4 expression modified on the cellular proliferation of bone marrow mesenchymal stem cells were assayed with flow cytometry. Effect of CXCR4 regulation on secretion function of bone marrow mesenchymal stem cells was determined by protein analysis from cell supernatant.
    RESULTS AND CONCLUSION: CXCR4 gene or GFP gene could be transfected into bone marrow mesenchymal stem cells safely and effectively with lentivirus transfection system. Analysis of CXCR4 expression at the mRNA and protein levels confirmed the transfection efficiency. Effects of CXCR4 over-expression on the cellular proliferation of bone marrow mesenchymal stem cells and effects of CXCR4 regulation on secretion of protective factors from bone marrow mesenchymal stem cells were improved. The proliferation ability of bone marrow mesenchymal stem cells with CXCR4 over-expression was improved, which was approved with flow cytometry (P < 0.05). And it was helpful for bone marrow mesenchymal stem cells to secrete some protective factors. Many protein and soluble factors were at higher levels, and those were good at cells proliferation and immunoloregulation in bone marrow mesenchymal stem cells (P < 0.05). The method of lentiviral transfection is a safe and effective way to modify bone marrow mesenchymal stem cells. We can improve the ability of bone marrow mesenchymal stem cells in the survival and emiocytosis ability through genetic engineering.

    中国组织工程研究杂志出版内容重点:干细胞;骨髓干细胞;造血干细胞;脂肪干细胞;肿瘤干细胞;胚胎干细胞;脐带脐血干细胞;干细胞诱导;干细胞分化;组织工程


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    Vascular endothelial growth factor promotes the proliferation and osteogenic differentiation of mouse bone marrow mesenchymal stem cells
    Zhang Lian-fang, Kang Hui, Deng Lian-fu, Yang Hui-lin
    2014, 18 (41):  6591-6596.  doi: 10.3969/j.issn.2095-4344.2014.41.006
    Abstract ( 282 )   PDF (664KB) ( 608 )   Save

    BACKGROUND: Vascular endothelial growth factor (VEGF) is a crucial factor for regulating bone marrow mesenchymal stem cells in microenvironment, which can promote endothelial differentiation of bone marrow mesenchymal stem cells. But there is no report about VEGF effect on regulating the proliferation and osteogenic differentiation of bone marrow mesenchymal stem cells.

    OBJECTIVE: To investigate the regulatory role of VEGF in the proliferation and osteogenic differentiation of bone marrow mesenchymal stem cells.
    METHODS: The bone marrow mesenchymal stem cells were isolated and cultured from mouse long bones. Cell Counting Kit-8 was used to test the proliferation of bone marrow mesenchymal stem cells cultured with different concentrations of recombinant VEGF proteins. The appropriate concentration of VEGF recombinant protein was selected to test their effects on the osteogenic differentiation of bone marrow mesenchymal stem cells. The mRNA and protein levels of Osterix, Runx2, alkaline phosphatase, osteocalcin and heme oxygenase-1 in bone marrow mesenchymal stem cells under induction of VEGF were detected by molecular biology methods.
    RESULTS AND CONCLUSION: (1) VEGF promoted the proliferation of bone marrow mesenchymal stem cells dose-dependently, and 100 μg/L was the optimum concentration. (2) VEGF promoted the expression of Osterix, Runx2, alkaline phosphatase and osteocalcin in bone marrow mesenchymal stem cells under osteogenic induction. Similar results were obtained by alizarin red staining and quantification of numbers of calcium nodules. (3) VEGF induced the expression of heme oxygenase-1 in bone marrow mesenchymal stem cells at mRNA and protein levels. These findings indicate that VEGF can induce the expression of heme oxygenase-1 i in bone marrow mesenchymal stem cells, and promote the proliferation and osteogenic differentiation of bone marrow mesenchymal stem cells.

    中国组织工程研究杂志出版内容重点:干细胞;骨髓干细胞;造血干细胞;脂肪干细胞;肿瘤干细胞;胚胎干细胞;脐带脐血干细胞;干细胞诱导;干细胞分化;组织工程


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    Construction of cardiac atrioventricular electrical conduction pathway by rabbit bone marrow mesenchymal stem cells
    Zhou Hao-yue, Lu Jiong-bin, Qiu Han-ying
    2014, 18 (41):  6597-6602.  doi: 10.3969/j.issn.2095-4344.2014.41.007
    Abstract ( 309 )   PDF (892KB) ( 453 )   Save

    BACKGROUND: Autologous stem cell transplantation to the heart is a research direction of heart failure treatment, but there are relatively few of studies about autologous bone marrow mesenchymal stem cell transplantation targeting the cardiac conduction system.

    OBJECTIVE: To evaluate the potential of rabbit bone marrow mesenchymal stem cells for treatment of heart block.
    METHODS: Rabbit bone marrow mesenchymal stem cells were induced by 5-azacytidine to differentiate into cardiomyocyte-like cells. After thoracotomy, the left atrium-left ventricular anterior wall was sutured in 14 New Zealand white rabbits (8 in the experimental group and 6 in the control group). One month after the surgery, in the experimental group, autologous bone marrow mesenchymal stem cells induced by 5-azacytidine for 4 weeks were labeled with 4',6-diamidino-2-phenylindole and then injected into the suture area when opening the thoracic again. In the control group, cells cultured in medium were used. One month after cell transplantation, the third thoracotomy was done to insert electrodes into the left atrium and left ventricular anterior wall, for cardiac electrophysiological detection. Whether atrioventricular pathway formed in the suture area was observed.
    RESULTS AND CONCLUSION: After cells were transplanted into the sutured area, two rabbits in the experimental group were discovered to form the atrioventricular pathway in the sutured area through cardiac electrophysiological examination. After transplantation, transplanted cells were visible on the heart frozen sections under fluorescence microscope in the left ventricle and sutured area, but there was no cell in the control group. In the experimental group, bone marrow mesenchymal stem cells expressed Cx43 and formed gap junction intercellular communication with cardiomyocytes, which was presented as formation of the atrioventricular pathway on cardiac electrophysiology examination. These findings indicate that bone marrow mesenchymal stem cells can be used to treat cardiac conduction system block diseases.

    中国组织工程研究杂志出版内容重点:干细胞;骨髓干细胞;造血干细胞;脂肪干细胞;肿瘤干细胞;胚胎干细胞;脐带脐血干细胞;干细胞诱导;干细胞分化;组织工程


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    Let-7d lentiviral vector induces the differentiation of rat bone marrow mesenchymal stem cells into neurons in vitro
    Xu Xiao-ge, Zhang Jing, Gong Zhe, Zhao Shao-yun, He Xia, Wang Tian-shu, Jiao Shu-jie, Teng Jun-fang, Jia Yan-jie
    2014, 18 (41):  6603-6608.  doi: 10.3969/j.issn.2095-4344.2014.41.008
    Abstract ( 303 )   PDF (863KB) ( 590 )   Save

    BACKGROUND: MicroRNA plays an important role in the process of growth and aging of living body. To know the role of let-7d in inducing bone marrow mesenchymal stem cell differentiation into neurons can promote the stem cell transplantation.

    OBJECTIVE: To investigate the role of let-7d in inducing bone marrow mesenchymal stem cell differentiation into neurons.
    METHODS: (1) The lentiviral vector of let-7d was constructed and transfected into rat bone marrow mesenchymal stem cells. The cells were divided into non-transfected group, negative control group (transfected with FU-RNAi-NC-LV), transfected enhancement group (transfected with let-7d-LV), transfected inhibition group ( transfected with let-7d-inhibition-LV). (2) Rat bone marrow mesenchymal stem cells were treated with fasudil as an inducer for triggering the cells to differentiate into neurons. The expression of neuron-specific markers, neuron-specific enolase and microtubule-associated protein 2, were measured by immunocytochemical method. The mRNA expression of microtubule-associated protein 2 was detected by RT-PCR. The viability of bone marrow mesenchymal stem cells was determined by MTT method.
    RESULTS AND CONCLUSION: Under inverted fluorescence microscope, the cells were successfully transfected with let-7d. Fasudil induced bone marrow mesenchymal stem cells to differentiate into neurons. The transfection efficiency and expression levels of neuron-specific enolase and microtubule-associated protein 2 in transfected enhancement group were higher than those in the negative control group (P < 0.05); while in the inhibition group, they were lower than those in the negative control group (P < 0.05). These findings indicate that let-7d can promote the differentiation of bone marrow mesenchymal stem cells into neurons induced by fasudil, and by controlling the expression of let-7d we can influence the differentiation efficiency from bone marrow mesenchymal stem cells to neurons.

    中国组织工程研究杂志出版内容重点:干细胞;骨髓干细胞;造血干细胞;脂肪干细胞;肿瘤干细胞;胚胎干细胞;脐带脐血干细胞;干细胞诱导;干细胞分化;组织工程


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    The different methods to induce the osteogenic differentiation of rabbit bone marrow mesenchymal stem cells
    Wang Hui, Zhang Jian-jun
    2014, 18 (41):  6609-6613.  doi: 10.3969/j.issn.2095-4344.2014.41.009
    Abstract ( 287 )   PDF (803KB) ( 561 )   Save

    BACKGROUND: At present, heterologous serum as a medium is a common method for culture of bone marrow mesenchymal stem cells, but it is limited by the disease transmission between species, the potential immune rejection and even controversial ethic issues. This method is also contrary to the requirements of the Ministry of Health. Autologous platelet-rich plasma is a kind of whole blood extract, containing a variety of growth factors.

    OBJECTIVE: To explore the osteogenic differentiation of rabbit bone marrow mesenchymal stem cells cultured in autologous platelet rich plasma alternative to traditional heterogeneous serum-free culture.
    METHODS: 8 mL bone marrow from the rabbit iliac crest was extracted and anti-coagulated with heparin, and then bone marrow mesenchymal stem cells were isolated using density gradient centrifugation. The cells were divided into autologous platelet rich plasma group (10% autologous platelet rich plasma) and fetal bovine serum group (10% fetal bovine serum). At the passage 4, the cells in the two groups were respectively subdivided into experimental and control groups. Experimental groups were subjected to osteogenic induction, while no change was done in the control groups. Cell proliferation was determined by using growth curves; the activity of alkaline phosphatase was detected to adjust the osteogenic differentiation of cells in different groups.
    RESULTS AND CONCLUSION: Bone marrow mesenchymal stem cells at different generations all showed good proliferation. At 12 days of autologous platelet rich plasma culture, the activity of alkaline phosphatase in the passage 4 cells in the experimental group was significantly higher than that in the control group; while the activity of alkaline phosphatase in the autologous platelet rich plasma group was significantly higher than that in the fetal bovine serum group (P < 0.01). These findings indicate that autologous platelet rich plasma as a substitute of xenogeneic serum for culture of bone marrow mesenchymal stem cells is a method characterized as safe and reliable, simple operation, high-purity active induction.

    中国组织工程研究杂志出版内容重点:干细胞;骨髓干细胞;造血干细胞;脂肪干细胞;肿瘤干细胞;胚胎干细胞;脐带脐血干细胞;干细胞诱导;干细胞分化;组织工程


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    Biological characteristics of human umbilical cord mesenchymal stem cells: cold trypsin digestion versus tissue explant method in vitro   
    Zhang Fei, Wang Yi-xiong, Wu Zhong-yan, Li Gui-cai, Cao Peng, Wang Wu
    2014, 18 (41):  6614-6619.  doi: 10.3969/j.issn.2095-4344.2014.41.010
    Abstract ( 318 )   PDF (787KB) ( 750 )   Save

    BACKGROUND: Cold trypsin digestion is rarely reported to culture umbilical cord mesenchymal stem cells.

    OBJECTIVE: To compare the biological characteristics of umbilical cord mesenchymal stem cells cultured by cold trypsin digestion and tissue explant method.
    METHODS: Human umbilical cord mesenchymal stem cells were isolated, purified and passaged using cold trypsin digestion and tissue explant method, and then the first adhesion time and cell cycle were recorded. Morphology of umbilical cord mesenchymal stem cells was observed under inverted phase contrast microscope to draw growth curve of cells at passage 3. Flow cytometry was used to detect the surface markers of passage 3 umbilical cord mesenchymal stem cells, and Nestin expression was detected in passage 3 cells after 3 days culture in neural induction medium by fluorescence immunochemistry staining.
    RESULTS AND CONCLUSION: These two methods were both successful to harvest umbilical cord mesenchymal stem cells, but the first adhesion time was earlier in cells cultured by cold trypsin digestion than tissue explant method (P < 0.05), and there was no difference in primary cell cycle (P > 0.05). Under the inverted  
    microscope, cells grew adherently and presented fibroblast-like shape. However, the minority of primary cells under induction of cold trypsin digestion was polygonal, irregular, and had larger cell volume than those cultured by tissue explant method. Passage 3 cells cultured by tissue explant method showed faster proliferation rate than those cultured by cold trypsin digestion, and at logarithmic growth phase, cells cultured by these two methods were significant different in cell number (P < 0.05). Two types of cells had a uniform cell phenotype, both of which expressed CD29, CD105, but did not express CD34, CD45. Under induction, passage 3 cells cultured by these two methods were both positive for nestin. These findings indicate that these two methods can both be used to culture umbilical cord mesenchymal stem cells, but the tissue explant method is more suitable for culture of umbilical cord mesenchymal stem cells and exhibits less damage to cells.

    中国组织工程研究杂志出版内容重点:干细胞;骨髓干细胞;造血干细胞;脂肪干细胞;肿瘤干细胞;胚胎干细胞;脐带脐血干细胞;干细胞诱导;干细胞分化;组织工程


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    Security of heterogeneous umbilical cord mesenchymal stem cells via intramuscular injection in Wistar rats
    Zhang Wen-xiang, Wang Si-ping, Xi Hong-min, Li Zi-pu
    2014, 18 (41):  6620-6627.  doi: 10.3969/j.issn.2095-4344.2014.41.011
    Abstract ( 313 )   PDF (2744KB) ( 395 )   Save

    BACKGROUND: So far, the short-term changes of various organs after injection of umbilical cord mesenchymal stem cells have been reported, but there are few studies on the long-term changes of various organs in healthy rats after repeated intramuscular injection of umbilical cord mesenchymal stem cells.

    OBJECTIVE: To observe the security of intramuscular injection of heterogeneous umbilical cord mesenchymal stem cells.
    METHODS: Sixty male SPF Wistar rats were divided into six groups randomly: normal group (suspension liquid of umbilical cord mesenchymal stem cells); control group with culture solution; supernatant group (supernatant of human umbilical cord mesenchymal stem cells); low concentration group (0.25×105 human umbilical cord mesenchymal stem cells); moderate concentration group (1.0×105 human umbilical cord mesenchymal stem cells); high concentration group (4.0×105 human umbilical cord mesenchymal stem cells). Each rat was injected 0.8 mL liquid in muscle, 0.2 mL in each limb, twice at weeks 1 and 4. Biochemical tests were conducted before and after injection. At the end of 8 weeks, all the rats were killed and hematoxylin-eosin staining was done with the liver, spleen, lung, kidney, brain and muscle.
    RESULTS AND CONCLUSION: There was no abnormal change about biochemical tests and hematoxylin-eosin staining after the intramuscular injection of heterogeneous umbilical cord mesenchymal stem cells. No significant alteration was observed in the liver, spleen, lung, kidney, brain, and muscle of the limb after the injection of heterogeneous umbilical cord mesenchymal stem cells under suitable concentration. These findings indicate intramuscular injection of heterogeneous umbilical cord mesenchymal stem cells at certain concentrations is safe and reliable.

    中国组织工程研究杂志出版内容重点:干细胞;骨髓干细胞;造血干细胞;脂肪干细胞;肿瘤干细胞;胚胎干细胞;脐带脐血干细胞;干细胞诱导;干细胞分化;组织工程


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    Survival of bone marrow mesenchymal stem cells after transplantation into the rat infarcted myocardium
    Lin Chu-wei, Zhou Sheng-hua, Dai Hai-ying, Deng Ping, Huang Hong-guang, Yin Zhi-lan, Guan Xian-song
    2014, 18 (41):  6628-6632.  doi: 10.3969/j.issn.2095-4344.2014.41.012
    Abstract ( 345 )   PDF (1553KB) ( 468 )   Save

    BACKGROUND: The preliminary findings confirm that bone marrow mesenchymal stem cell transplantation is safe and effective in the treatment of acute myocardial infarction, but its exact mechanism is unclear. There are few studies addressing the survival status of transplanted stem cells and its acting timing.

    OBJECTIVE: To study the survival of rat bone marrow mesenchymal stem cells transplanted into the infracted myocardium.
    METHODS: Bone marrow mesenchymal stem cells were cultured using density gradient centrifugation. Eighty rat models of myocardial infarction were prepared. Bone marrow mesenchymal stem cells were injected via a microsyringe at four sites around the infarcted region at 14 days after modeling. Then, 70 rats with living cells were selected for detecting the survival of bone marrow mesenchymal stem cells at days 3, 5, 7, 10, 20, 28 after transplantation.

    RESULTS AND CONCLUSION: Under ×400 visual field, the number of Brdu-positive bone marrow mesenchymal stem cells was (36±12) at 3 days posttranplantation, (33±13) at 5 days, (28±9) at 7 days, (15±5) at 10 days, (5±3) at 14 days, 0 at 20 days, and 0 at 28 days, showing a overall downward trend after transplantation. The number of bone marrow mesenchymal stem cells was negatively correlated with transplant days (P < 0.01, r=-0.47). The number of cells decreased most significantly within 1 week after transplantation, and then decreased to 0 at 20 days. These findings indicate that transplanted bone marrow mesenchymal stem cells in the myocardium cannot survive for a long term and also cannot be transformed into myocardial tissue.


    中国组织工程研究杂志出版内容重点:干细胞;骨髓干细胞;造血干细胞;脂肪干细胞;肿瘤干细胞;胚胎干细胞;脐带脐血干细胞;干细胞诱导;干细胞分化;组织工程


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    Platelet-rich plasma plus human umbilical cord mesenchymal stem cells for cartilage repair
    Xu Jing1, Wang Li-ming, Zhou Li-dong, Wu Mei, Cui Hui, Zhao Jing, Zeng Du-juan, Zhang Zhong-wen, Liu Ai-bing
    2014, 18 (41):  6633-6638.  doi: 10.3969/j.issn.2095-4344.2014.41.013
    Abstract ( 387 )   PDF (1978KB) ( 620 )   Save

    BACKGROUND: Chondrocytes co-cultured with bone marrow stromal stem cells on the scaffold of platelet-rich plasma are found to proliferate, and besides proliferative growth, bone marrow stromal cells exhibit a tendency of differentiating into chondrocytes.

    OBJECTIVE: To study the effect of platelet-rich plasma and human umbilical cord mesenchymal stem cells (hUCMSCs) on cartilage repair.
    METHODS: Forty healthy New Zealand white rabbits were selected to establish models of cartilage defects, and then randomly divided into normal saline group, platelet-rich plasma group, hUCMSCs group and combination group. Platelet-rich plasma was prepared by using double centrifugations to prepare passage 3 hUCMSCs. After modeling, intra-articular injection of normal saline (0.5 mL), 12.5% platelet-rich plasma (0.5 mL), 1×107 hUCMSCs (0.5 mL), 12.5% platelet-rich plasma+1×107 hUCMSCs (totally 0.5 mL) was done in corresponding groups, respectively. After 12 weeks of modeling, the injured cartilage was grossly observed, and hematoxylin-eosin staining was used to observe cartilage repair under light microscope; according to the O'Driscoll histologic standard, histological examination was performed.

    RESULTS AND CONCLUSION: The repair effect in the normal saline group was significantly better that in the platelet-rich plasma group, hUCMSCs group, combination group (P < 0.05), while the platelet-rich plasma group and combination group also exhibit better outcomes than the hUCMSCs group (P < 0.05). These findings indicate that both platelet-rich plasma and hUCMSCs can promote cartilage repair; moreover, platelet-rich plasma with or without hUCMSCs is superior to hUCMSCs alone in the cartilage repair.


    中国组织工程研究杂志出版内容重点:干细胞;骨髓干细胞;造血干细胞;脂肪干细胞;肿瘤干细胞;胚胎干细胞;脐带脐血干细胞;干细胞诱导;干细胞分化;组织工程


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    Cistanche deserticola plus bone marrow mesenchymal stem cell transplantation for treating spinal cord injury in rats
    Lan Jing, Yan Jin-yu, Xia Run-fu, Li Jian-feng
    2014, 18 (41):  6639-6644.  doi: 10.3969/j.issn.2095-4344.2014.41.014
    Abstract ( 266 )   PDF (731KB) ( 530 )   Save

    BACKGROUND: Studies have shown that bone marrow mesenchymal stem cells under certain conditions can differentiate into nerve cells. Bone marrow mesenchymal stem cell transplantation can rebuild nervous system function and improve functional disorders in patients. Glycosides of cistanche also have a protective effect against nerve cell injury. Their combination has been less reported.

    OBJECTIVE: To observe the effectiveness of Cistanche deserticola and bone marrow mesenchymal stem cell transplantation on spinal cord injury in rats. 
    METHODS: Fifty adult Wistar rats with spinal cord injury were randomly divided into four group: a Cistanche deserticola group (intragastric administration of 20 mL/kg Cistanche deserticola concentrated solution per day), a cell transplantation group (10 μL of 1×108/L bone marrow mesenchymal stem cell suspension), a combination group (10 μL of 1×108/L bone marrow mesenchymal stem cell suspension+ intragastric administration of
    20 mL/kg Cistanche deserticola concentrated solution per day) and a control group (intragastric administration of 20 mL/kg normal saline per day). The intragastric administration lasted for 30 days in each group.

    RESULTS AND CONCLUSION: After 30 days of treatment, the expression of Nestin was significantly higher in the combination group than the other groups. After 12 weeks, Basso, Beattie, and Bresnahan scores was significantly higher in the combination group than the other groups (P < 0.05); somatosensory and motor evoked potential latencies were also improve significantly in the combination group compared with the other groups (P < 0.05). These findings indicate that oral administration of Cistanche deserticola combined with bone marrow mesenchymal stem cells can significantly improve the motor and neurophysiological function of spinal cord injury rats. Cistanche deserticola can improve the survival of transplanted bone marrow mesenchymal stem cells in rats with spinal cord injury.


    中国组织工程研究杂志出版内容重点:干细胞;骨髓干细胞;造血干细胞;脂肪干细胞;肿瘤干细胞;胚胎干细胞;脐带脐血干细胞;干细胞诱导;干细胞分化;组织工程


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    Blood glucose recovery following allogenetic hematopoietic stem cell transplantation in patients with hematological diseases complicated with diabetes mellitus
    Feng Kai, Xu Yi-wei, Ye Fu-guang, Jiang Min, Chen Hu, Shi Bing-yi
    2014, 18 (41):  6645-6648.  doi: 10.3969/j.issn.2095-4344.2014.41.015
    Abstract ( 307 )   PDF (508KB) ( 551 )   Save

    BACKGROUND: Recent studies have shown that the large-dose regular insulin therapy used to control blood glucose levels can cause 50% of patients suffering from vascular, optic nerve and kidney complications. Previous results from authors exhibit that when allogeneic hematopoietic stem cell transplantation is applied for treatment of leukemia, diabetic symptoms in patients disappear. Dose it prompt that allogeneic hematopoietic stem cell transplantation is an effective therapy for treatment of diabetes mellitus?

    OBJECTIVE: To explore the feasibility of hematopoietic stem cell transplantation for treatment of diabetes mellitus.
    METHODS: A retrospective analysis was done regarding the data of patients with hematological diseases complicated with diabetes mellitus who underwent allogenetic hematopoietic stem cell transplantation. Four patients with acute lymphocyte leukemia, chronic myelogenous leukemia, aplastic anemia, and myolodysplastic syndromes, respectively, were complicated with diabetes mellitus. Conditioning regimen was cyclophosphamide+ total body irradiation protocol. Cyclosporin A and short-term methotrexate were used for graft-versus-host disease prophylaxis. Blood glucose was controlled by oral hypoglycemic drugs or insulin injections before transplantation.
    RESULTS AND CONCLUSION: All the four patients were successfully engrafted. Fasting glucose level of the four patients recovered at 4-6 months after hematopoietic stem cell transplantation (without hypoglycemic drugs). One patient died of leukemia relapse after 12 months of hematopoietic stem cell transplantation. The other three  patients had disease-free survival until the time of follow-up.

    中国组织工程研究杂志出版内容重点:干细胞;骨髓干细胞;造血干细胞;脂肪干细胞;肿瘤干细胞;胚胎干细胞;脐带脐血干细胞;干细胞诱导;干细胞分化;组织工程


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    Drynaria freeze-dried powder at different dosages influences proliferation and differentiation of rabbit bone marrow mesenchymal stem cells
    Zhang Hu-lin, Zhang Zi-qiang, Xu Hong-bin, Zhang Xiao-gang
    2014, 18 (41):  6649-6654.  doi: 10.3969/j.issn.2095-4344.2014.41.016
    Abstract ( 243 )   PDF (865KB) ( 654 )   Save

    BACKGROUND: Modern research shows that Drynaria can delay cell degeneration and reduce the incidence of osteoarthritis.

    OBJECTIVE: To observe the proliferation and differentiation of rabbit bone marrow mesenchymal stem cells induced by different dosages of Drynaria freeze-dried powder, and to explore the optimum induction concentration.
    METHODS: Rabbit bone marrow mesenchymal stem cells were isolated and cultured in vitro by density gradient centrifugation and adherence screening methods, and then divided into blank group, positive control group (transforming growth factor β1), high-, middle-, low-dosage Drynaria groups (0.4 mg, 0.1 mg, 5 μg). Passage 3 cells were selected and cultured in different media. After 1 week, cell viability was detected by MTT method, and expression of type II collagen by immunohistochemical method.
    RESULTS AND CONCLUSION: Both transforming growth factor β1 and Drynaria could improve the proliferation of bone marrow mesenchymal stem cells, and the increase in cell proliferation was ranked as follows: positive control group > low-dosage group > middle-dosage group > high-dosage group > blank group. Bone marrow mesenchymal stem cells were differentiated into chondrocytes under induction of transforming growth factor β1 and Drynaria, and induced cells significantly expressed type II collagen. The expression of type II collagen was ranked as follows: positive control group > low-dosage group > middle-dosage group > high-dosage group > blank group. These findings suggest that Drynaria can promote the proliferation and differentiation of rabbit bone marrow mesenchymal stem cells, and the optimal dosage is 5 μg.

    中国组织工程研究杂志出版内容重点:干细胞;骨髓干细胞;造血干细胞;脂肪干细胞;肿瘤干细胞;胚胎干细胞;脐带脐血干细胞;干细胞诱导;干细胞分化;组织工程


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    Biocompatibility of annulus fibrosus-derived stem cells with porcine decellularized annulus fibrosus matrix
    Liu Chen, Wang Sheng-hao, Wang Yi-bin, Tang Xue-bin, Yang Hui-lin, Li Bin
    2014, 18 (41):  6655-6660.  doi: 10.3969/j.issn.2095-4344.2014.41.017
    Abstract ( 317 )   PDF (765KB) ( 503 )   Save

    BACKGROUND: Acellular matrix and annulus fibrosus-derived stem cells are both derived from the annulus tissue, and their tissue engineering complexes may have better biocompatibility.

    OBJECTIVE: To culture rabbit annulus fibrosus-derived stem cells on the porcine decellularized annulus fibrosus matrix, and to observe the growth of annulus fibrosus-derived stem cells on the decellularized matrix scaffold.
    METHODS: Decellularized annulus fibrosus matrix from porcine was prepared and detected by scanning electron microscopy, DAPI staining and Fourier transform infrared spectroscopy analysis. After isolation and culture, annulus fibrosus-derived stem cells were seeded onto the decellularized annulus fibrosus matrix. Cell growth on the scaffolds was observed by cytoskeleton staining, inverted immunofluorescence microscopy and scanning electron microscopy to draw cell growth curve.
    RESULTS AND CONCLUSION: The prepared decellularized annulus fibrosus matrix was white and translucence liquid. The result of Fourier transform infrared spectroscopy indicated that the main component of the decellularized annulus fibrosus matrix was collagen. DAPI staining and scanning electron microscopy results showed no cells resident. The cytoskeleton staining displayed that annulus fibrosus-derived stem cells grew well on the scaffolds at 1, 3, 7 days. These findings indicated that annulus fibrosus-derived stem cells have a good biocompatibility with the decellularized annulus fibrosus matrix in vitro.

    中国组织工程研究杂志出版内容重点:干细胞;骨髓干细胞;造血干细胞;脂肪干细胞;肿瘤干细胞;胚胎干细胞;脐带脐血干细胞;干细胞诱导;干细胞分化;组织工程


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    Relationship between CD4+CD25+FOXP3+Treg cells and acute graft-versus-host disease
    Xie Xin-sheng, Wan Ding-ming, Sun Hui, Sun Ling, Zhang Qiu-tang
    2014, 18 (41):  6661-6665.  doi: 10.3969/j.issn.2095-4344.2014.41.018
    Abstract ( 263 )   PDF (624KB) ( 720 )   Save

    BACKGROUND: The CD4+CD25+FOXP3+Treg cells have immunosuppression effect, and it is speculated that these cells may restrain the occurrence of acute graft-versus-host disease.

    OBJECTIVE: To observe the variety of the CD4+CD25+FOXP3+Treg cells in the peripheral blood from donors before and after granulocyte colony stimulating factor mobilization, and study the relationship between CD4+CD25+FOXP3+Treg cells and acute graft-versus-host disease.
    METHODS: Ninety patients with malignant blood diseases who undertook allogeneic hematopoietic stem cell transplantation and their donors were observed. Granulocyte colony stimulating factor 5 μg/kg was injected subcutaneously into the donor per 12 hours for 5 days, and the stem cells were collected before and after mobilization. The ratio of CD4+CD25+ FOXP3+Treg cells in the peripheral blood was detected before and after mobilization with flow cytometry, and the ratio of these cells in the stem cell suspension was measured by the same method. The patients were divided into two groups according to the CD4+CD25+ FOXP3+Treg cells ratio: high dosage group (≥5%) and low dosage group (< 5%). The incidence of acute graft-versus-host disease was observed in the two groups after transplantation.
    RESULTS AND CONCLUSION: The ratio of the CD4+CD25+FOXP3+Treg cells in the donor before and after mobilization were 11.3% and 1.5%,respectively, and there was a significant difference (P < 0.05). The ratio of the CD4+CD25+FOXP3+Treg cells was 3.4% in the patients with acute graft-versus-host disease, and 15.7% in the patients with no acute graft-versus-host disease, showing a significant difference (P < 0.05). After hematopoietic reconstitution, the incidence of acute graft-versus-host disease was 18.4% in the high dosage group and 48.1% in the low dosage group, and there was a significant difference between the two groups (P < 0.05). Therefore, the granulocyte colony stimulating factor can lower the ratio of CD4+CD25+FOXP3+Treg cells in the human peripheral blood. The increase in CD4+CD25+FOXP3+Treg cells can restrain the occurrence of acute graft-versus-host disease.

    中国组织工程研究杂志出版内容重点:干细胞;骨髓干细胞;造血干细胞;脂肪干细胞;肿瘤干细胞;胚胎干细胞;脐带脐血干细胞;干细胞诱导;干细胞分化;组织工程


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    Intrathecal injection of umbilical cord mesenchymal stem cells for spinocerebellar ataxia
    Liu Jing, Han Dong-mei, Ding Li, Xue Mei, Yan Hong-min, Wang Zhi-dong, Zhu Ling, Zheng Xiao-li, Dong Lei, Guo Zi-kuan, Wang Heng-xiang
    2014, 18 (41):  6666-6670.  doi: 10.3969/j.issn.2095-4344.2014.41.019
    Abstract ( 352 )   PDF (1896KB) ( 841 )   Save

    BACKGROUND: Spinocerebellar ataxia is a inherited neurodegenerative disease with progressive cerebellar masonic movement disorders as the main clinical manifestation. So far, no drug is available to control the disease progression.

    OBJECTIVE: To observe the clinical effect of umbilical cord mesenchymal stem cells in treating spinocerebellar ataxia by intrathecal injection.
    METHODS: Thirty-eight cases of spinocerebellar ataxia were given umbilical cord mesenchymal stem cells by intrathecal injection, 1×106/kg once a week, four times as a course. These 38 cases received 52 courses. Among them, 27 cases received 1 course, 8 cases received 2 courses and 3 cases received 3 courses. International Cooperative Ataxia Rating Scale (ICARS) and Activity of Daily Living Scale (ADL) were used to evaluate patients’ neural functions (the greater scores, the more severe damage) and ability of daily living (the lower score, the stronger the ability of daily living). After treatment, all patients were subjected to follow-up visit.

    RESULTS AND CONCLUSION: The total effective rate of 52 courses of treatment was 84.62%. ICARS and ADL scores were significantly decreased at 1 month after treatment (P < 0.01). In most of effective patients, unstable walking and standing, slow movement, upper limb fine motor disorder, writing difficulties, dysarthria, eye movement disorders were improved. After treatment, common adverse effects were dizziness (1 case), low back pain (2 cases), headache (1 case), and fever (2 cases). All these symptoms disappeared within 1-3 days. No treatment-related adverse events happened in the median follow-up of 39 months (11-59 months). The illness of effective patients had been stable for 1-19 months, average (5.95±4.84) months. Intrathecal injection of umbilical cord mesenchymal stem cells is safe to ameliorate clinical symptoms to some extent within a certain time. It may delay the progression of spinocerebellar ataxia. Multiple courses of treatment can help to further improve neurological function in most patients.


    中国组织工程研究杂志出版内容重点:干细胞;骨髓干细胞;造血干细胞;脂肪干细胞;肿瘤干细胞;胚胎干细胞;脐带脐血干细胞;干细胞诱导;干细胞分化;组织工程


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    Effects of human umbilical cord mesenchymal stem cells via intramuscular injection on the myocardial ki-67, phh3 and cTnT expression in normal rats
    Wang Qi, Zhang Wen-xiang, Wang Si-ping, Li Zi-pu
    2014, 18 (41):  6671-6677.  doi: 10.3969/j.issn.2095-4344.2014.41.020
    Abstract ( 424 )   PDF (2258KB) ( 639 )   Save

    BACKGROUND: It is unclear whether intramuscular injection of human umbilical cord mesenchymal stem cells will increase regeneration of normal myocardial cells and play adverse effects on normal myocardium.

    OBJECTIVE: To observe the effects of intramuscular injection of human umbilical cord mesenchymal stem cells on expression of the myocardial ki-67, phh3 and cTnT in normal Wistar rats.
    METHODS: A total of 60 male Wistar rats were divided into six groups randomly: normal group, solution group, supernatant group, low concentration group, moderate concentration group, and high concentration group. These groups were intramuscularly injected different liquid, respectively, as follows: PBS, DMEM, the supernatant of human umbilical cord mesenchymal stem cells, human umbilical cord mesenchymal stem cells with amount of 0.25×105, human umbilical cord mesenchymal stem cells with amount of 1.0×105, human umbilical cord mesenchymal stem cells with amount of 4.0×105. After 4 weeks, each rats received the second same intramuscular injections of human umbilical cord mesenchymal stem cells. Four weeks after the second injection, all rates were killed to obtain myocardial tissues which were fixed, embedded and sectioned. Finally, the ki-67, phh3 and cTnT expressions of the myocardium were detected by immunohistochemical technique. 
    RESULTS AND CONCLUSION: In the normal group, negative expression of the ki-67 was observed in the caryon of myocardial cells, weak positive expression of the phh3 was observed in the caryon of myocardial cells, and positive expression of the cTnT was observed in the caryon of myocardial cells. Compared with the normal group, the expression of the ki-67, phh3 and cTnT in the myocardium had no significant difference among the
    other groups (F=1.076, 0.167, 0.300; P > 0.05). Human umbilical cord mesenchymal stem cells or the supernatant of human umbilical cord mesenchymal stem cells via intramuscular injection have no effects on the expression of the ki-67, phh3 and cTnT in normal Wistar rats.

    中国组织工程研究杂志出版内容重点:干细胞;骨髓干细胞;造血干细胞;脂肪干细胞;肿瘤干细胞;胚胎干细胞;脐带脐血干细胞;干细胞诱导;干细胞分化;组织工程


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    Umbilical cord blood neural stem cells for obsolete spinal cord injury    
    Wu Yue-kui, Wang Shang-wu, Ma Jian-hua, Yi Bo, Gao Bing-bing, Qin Jia-zhen, Yang Zhi-jun, Dai Yi-wu
    2014, 18 (41):  6678-6683.  doi: 10.3969/j.issn.2095-4344.2014.41.021
    Abstract ( 336 )   PDF (2333KB) ( 530 )   Save

    BACKGROUND: With the medical development, prognostic outcomes of spinal cord injuries have not been improved significantly, and most patients also suffer from severe complications. Nowadays, lots of laboratories and clinical researches have suggested that cell therapy has a great potential, especially the application of umbilical cord blood stem cells in nervous system diseases.

    OBJECTIVE: To explore the feasibility and clinical effect of umbilical cord blood neural stem cells transplantation for patients with obsolete spinal cord injury.
    METHODS: Umbilical cord blood was harvested from newborns under aseptic condition, and differentiated into neural stem cells in vitro that were prepared into cell suspension at a concentration of 109/L. The cell suspension (3 mL) was injected via the L3-4 or L4-5 into the subarachnoid space. American Spinal Injury Association (ASIA) scores and the residual urine were assessed before and 3 months after transplantation.
    RESULTS AND CONCLUSION: After transplantation, all the patients showed a stable life indication. Three months later, ASIA scores were increased and the residual urine decreased, which significantly differed from those before transplantation (P < 0.05). These findings indicate that umbilical cord blood neural stem cells transplantation is a new treatment that can improve the limb function and life quality of patients with obsolete spinal cord injury.

    中国组织工程研究杂志出版内容重点:干细胞;骨髓干细胞;造血干细胞;脂肪干细胞;肿瘤干细胞;胚胎干细胞;脐带脐血干细胞;干细胞诱导;干细胞分化;组织工程


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    Retinoic acid, testosterone or their combination affects the cell cycle of adipose-derived stem cells
    Duan Fu-hua, Zeng Wen-qin, Yang Chun, Yang Hui-ying, Yu Mei-chun, Tao Hui, Dai Jing-xing, Yuan Lin
    2014, 18 (41):  6684-6688.  doi: 10.3969/j.issn.2095-4344.2014.41.022
    Abstract ( 318 )   PDF (812KB) ( 681 )   Save

    BACKGROUND: The researches about the effect of retinoic acid on the proliferation of adipose-derived stem cells are rare, and the researches on the testosterone are mainly on the inhibition of cell aging.

    OBJECTIVE: To study the effects of retinoic acid and testosterone or combination on the cell cycle of adipose derived stem cells.
    METHODS: Adipose derived stem cells were isolated from adult female Sprague Dawley rats with 2 months age and cultured in vitro till passage 3 adipose derived stem cells, and then the 3rd passage adipose-derived stem cells were performed with adipogenic induction, osteogenic induction and surface marker identification. The cells were divided into six groups: (1) Control group; (2) 10-5 mol/L retinoic acid group; (3) Retinoic acid group; (4)     10-5 mol/L retinoic acid+testosterone group; (5) 10-6 mol/L retinoic acid+testosterone group; (6) Testosterone group. The adipose-derived stem cells in the control group were cultured with Dulbecco’s modified Eagle’s medium+10% fetal bovine serum culture medium, and the adipose-derived stem cells in the other five groups were induced with corresponding dose of retinoic acid and testosterone on the basis of control group. After cultured for 36 hours, the flow cytometry was used to detect the changes of cell cycle.
    RESULTS AND CONCLUSION: Compared with the control group, cell proportions in phase G1 of 10-5 mol/L retinoic acid group and 10-6 mol/L retinoic acid group were increased significantly, and the cell proportions in phase S were decreased. Compared with control group, the cell proportion in phase G1 of testosterone group was significantly reduced, and the cell proportion in phase S was increased. Compared with 10-5 mol/L retinoic acid group and 10-6 mol/L retinoic acid group, cell proportions in phase G1 of 10-5 mol/L retinoic acid+testosterone group and 10-6 mol/L retinoic acid+testosterone group were reduced significantly and the cell proportions in phase S were increased. Retinoic acid can inhibit the cell cycle of adipose-derived stem cells in phase G1, and delay the process of the cell cycle from phase G1 to phase S; while testosterone can promote the cell cycle of adipose-derived stem cells from phase G1 to phase S; the combination induction of retinoic acid and testosterone can accelerate the process of the cell cycle of adipose-derived stem cells from phase G1 to phase S.

    中国组织工程研究杂志出版内容重点:干细胞;骨髓干细胞;造血干细胞;脂肪干细胞;肿瘤干细胞;胚胎干细胞;脐带脐血干细胞;干细胞诱导;干细胞分化;组织工程


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    Bone marrow stem cell transplantation for improving heart function of patients with acute myocardial infarction: a systematic review
    Liu Yang, Li Xiao-yan, Chen Pan-pan, Li Jun
    2014, 18 (41):  6689-6695.  doi: 10.3969/j.issn.2095-4344.2014.41.023
    Abstract ( 284 )   PDF (726KB) ( 576 )   Save

    BACKGROUND: Although increasing studies have shown that autologous bone marrow stem cell transplantation can treat myocardial infarction, but there is a lack of large-scale multi-center randomized controlled trials to illustrate the therapeutic effectiveness of autologous bone marrow stem cell treatment for myocardial infarction.

    OBJECTIVE: To systematically evaluate the improvement of heart function in patients with myocardial infarction undergoing autologous bone marrow stem cell transplantation.
    METHODS: Cochrane Central Register of Controlled Trials (Central), MEDLINE, EMbase, PEDro (www.pedro.org.au), OpenSIGLE, National Technical Information Service (NTIS), CNKI, VIP database (VlP), Wanfang Data and Chinese biomedical literature database (CBM) were searched for the randomized controlled trials addressing bone marrow stem cell transplantation for heart function in patients with myocardial infarction. The bibliographies of the included studies were also searched. The time span was from database establishment to November 2012. The extracted data were analyzed by RevMan5.1.
    RESULTS AND CONCLUSION: A total of 14 randomized controlled trials were included. Results from the meta-analysis showed that the ejection fraction and cardiac output of patients undergoing bone marrow stem cell transplantation was significantly different from those of patients without cell transplantation (WMD=5.23, 95%CI (0.73, 9.72), P < 0.01; WMD=1.69, 95%CI (1.23, 2.16), P < 0.000 01). Current evidence has demonstrated that bone marrow stem cell transplantation can remarkably improve the ejection fraction and cardiac output of myocardial infarction patients, which can be clinically recommended for improvement of heart function. Due to the limitations of the included studies, more large-sample, multi-center high-quality randomized controlled trials are required to further verify the therapeutic methods and effectiveness.

    中国组织工程研究杂志出版内容重点:干细胞;骨髓干细胞;造血干细胞;脂肪干细胞;肿瘤干细胞;胚胎干细胞;脐带脐血干细胞;干细胞诱导;干细胞分化;组织工程


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    Osteogenic differentiation of adipose-derived stem cells on a composite scaffold in the repair of osteoporotic bone defects
    Huang Cheng-long, Xiao Jin-gang
    2014, 18 (41):  6696-6702.  doi: 10.3969/j.issn.2095-4344.014.41.024
    Abstract ( 307 )   PDF (635KB) ( 506 )   Save

    BACKGROUND: The traditional treatment methods for osteoporosis accompanied by bone defects, such as autogenous bone graft, allograft, biomaterial implants, have significant limitations. The regenerative medicine approach using adipose-derived stem cells as seed cells offers a new way for the repair of bone defects following osteoporosis.

    OBJECTIVE: To review the pathogenesis of osteoporosis and its impacts on the repair of bone defects, the signal pathway regulation of osteogenic differentiation of adipose-derived stem cells, and the feasibility of adipose-derived stem cells for repairing osteoporotic bone defects.
    METHODS: A computer-based online search of CNKI database and PubMed database was performed to retrieve the relevant articles published from January 1998 to September 2014 with the key words of “adipose-derived stem cells, osteoporosis, bone defect, osteogenic differentiation, bone regeneration” in Chinese and English, respectively. Finally 77 articles were included for review after deleting unrelated and repetitive ones.

    RESULTS AND CONCLUSION: In recent years, adipose-derived stem cells have been widely used in regenerative medicine research. With the development of relevant disciplines such as regenerative medicine, tissue engineering, molecular biology, and material science, in-depth studies on regulatory mechanisms of osteogenic differentiation of adipose-derived stem cells have been developed. Adipose-derived stem cells combined with biological scaffolds for construction of tissue-engineered bones provides a new way to repair osteoporotic bone defects.


    中国组织工程研究杂志出版内容重点:干细胞;骨髓干细胞;造血干细胞;脂肪干细胞;肿瘤干细胞;胚胎干细胞;脐带脐血干细胞;干细胞诱导;干细胞分化;组织工程


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    Bone marrow mesenchymal stem cells for repair of spinal cord injury: how to promote axonal regeneration?
    Li Hui-li, Du Cheng-fen, Zheng Hong-mei, Hou Ping-zhi, Wang Yun, Xiang Zi-jun, Lv Gui-li, Li Meng, Yu Hai-qin, Chen Shan-shan
    2014, 18 (41):  6703-6707.  doi: 10.3969/j.issn.2095-4344.2014.41.025
    Abstract ( 299 )   PDF (602KB) ( 466 )   Save

    BACKGROUND: Stem cells have been shown to not only replace damaged cells, but also secrete trophic factors, bringing a bright future for the treatment of clinical spinal cord injury.

    OBJECTIVE: To review the latest advances of bone marrow mesenchymal stem cells in animal and clinical research.
    METHODS: A computer-based search of Kjmed and Wanfang databases was done for relevant articles published from April 2004 to April 2014 using the keywords of “stem cells, spinal cord injuries, embryonic stem cells, neural stem cells, mesenchymal stem cells” in English and Chinese, respectively.

    RESULTS AND CONCLUSION: Totally 2 745 articles were initially retrieved, and only 50 articles were included in result analysis. Bone marrow mesenchymal stem cells have become one of the most promising sources of stem cells in the treatment of spinal cord injury. Although the bone marrow mesenchymal stem cell in the treatment of spinal cord injury is still in its infancy, it has certain effects on the repair of spinal cord injury. The mechanism of action of bone marrow mesenchymal stem cells in the treatment of spinal cord injury is possibly related to the substitution effect, neurotrophic effects, suppression of the immune response and promoting axonal regeneration.


    中国组织工程研究杂志出版内容重点:干细胞;骨髓干细胞;造血干细胞;脂肪干细胞;肿瘤干细胞;胚胎干细胞;脐带脐血干细胞;干细胞诱导;干细胞分化;组织工程


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    Mitochondrial transfer mechanism of bone marrow mesenchymal stem cells for rescue of tissue injury 
    Peng Yi, Shu Chang, Fu Zhou
    2014, 18 (41):  6708-6713.  doi: 10.3969/j.issn.2095-4344.2014.41.026
    Abstract ( 555 )   PDF (595KB) ( 659 )   Save

    BACKGROUND: Bone marrow mesenchymal stem cells have beneficial effects on the treatment of various diseases. Mitochondria transfer is newly proposed and its specific mechanisms of action and control factors remain unclear.

    OBJECTIVE: To review the studies about stem cells and mitochondrial transfer, then to discuss its value on clinical.
    METHODS: The PubMed, VIP and Wanfang databases were searched for related articles concerning stem cells and mitochondrial transfer. Key words were “stem cell, embryonic stem cell, progenitor cell, mitochondria” in Chinese and “Stem Cell[s], Mother cell[s], Progenitor cell[s], Colony-Forming Unit[s], Colony Forming Unit[s], Mitochondria[l] transfer” in English. Thirteen articles were found initially, and using the citation tracking method, finally 42 articles were determined.
    RESULTS AND CONCLUSION: Studies have shown that mitochondrial transfer from bone marrow mesenchymal stem cells is associated with rescue of aerobic respiration and restoration of mitochondrial function in the injured somatic cells. Bone marrow mesenchymal stem cells and recipient cells form tunneling nanotubes for mitochondrial transfer. Movement of mitochondria between cells is regulated and directed by Miro1. The successful transfer of mitochondria may be accompanied with the clearance of damaged mitochondria. The rescue of mitochondrial function in early stages may provide a valuable therapeutic strategy for various diseases include acute lung injury.

    中国组织工程研究杂志出版内容重点:干细胞;骨髓干细胞;造血干细胞;脂肪干细胞;肿瘤干细胞;胚胎干细胞;脐带脐血干细胞;干细胞诱导;干细胞分化;组织工程


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    Role of human umbilical cord mesenchymal stem cells: cell transplantation, immuoregulation and target cells 
    Ruan Guang-ping, Yao Xiang, Liu Ju-fen, Shu Fan, He Jie, Yang Jian-yong, Pang Rong-qing, Pan Xing-hua
    2014, 18 (41):  6714-6718.  doi: 10.3969/j.issn.2095-4344.2014.41.027
    Abstract ( 416 )   PDF (584KB) ( 654 )   Save

    BACKGROUND: Umbilical cord as childbirth waste has wide variety of sources and can be easily obtained, without any ethical and legal restrictions. Therefore, human umbilical cord mesenchymal stem cells break all the restrictions originated from other sources of mesenchymal stem cells.

    OBJECTIVE: To review the application of human umbilical cord mesenchymal stem cells in cartilage diseases, neuroglioma, ischemic brain injury, lung disease, liver disease and models of myocardial infarction.
    METHODS: The PubMed and Wanfang databases were searched by the first author using the keywords of “human umbilical cord mesenchymal stem cells, disease models, cell therapy” in English and Chinese, respectively. Seventy-three articles were searched and finally, 35 articles were included in result analysis.
    RESULTS AND CONCLUSION: Human umbilical cord mesenchymal stem cells have multilineage differentiation capacity similar to bone marrow mesenchymal stem cells. Compared with bone marrow mesenchymal stem cells, human umbilical cord mesenchymal stem cells have lower immunogenicity. Human umbilical cord mesenchymal stem cells show certain curative effects on cartilage disease, neuroglioma, ischemic brain injury, lung disease, liver disease and myocardial infarction, indicating that human umbilical cord mesenchymal stem cells can be used for cell transplantation to treat various diseases.

    中国组织工程研究杂志出版内容重点:干细胞;骨髓干细胞;造血干细胞;脂肪干细胞;肿瘤干细胞;胚胎干细胞;脐带脐血干细胞;干细胞诱导;干细胞分化;组织工程


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    Proliferation, senescence and differentiation of mesenchymal stem cells: canonical and non-canonical regulations of Wnt signaling pathway  
    Shi Jian-ming, Wu Ya-hua, Geng Shu-guo, Yin Ming
    2014, 18 (41):  6719-6724.  doi: 10.3969/j.issn.2095-4344.2014.41.028
    Abstract ( 492 )   PDF (699KB) ( 1214 )   Save

    BACKGROUND: As mesenchymal stem cells are commonly used as seed cells in studies of regenerative medicine and tissue engineering, the regulatory mechanism of their biological characteristics is a current research focus.

    OBJECTIVE: To summarize the regulations of Wnt signaling pathway on proliferation, senescence and differentiation of mesenchymal stem cells.
    METHODS: PubMed database and CNKI database were retrieved by computer using the key words of “mesenchymal stem cells, Wnt signaling pathway, proliferation, senescence, differentiation” in Chinese and English, respectively, between 2002 and 2014. Finally, 44 articles were included in result analysis.
    RESULTS AND CONCLUSION: Wnt signaling pathway is widely involved in the regulations of the biological characteristics of mesenchymal stem cells. Canonical Wnt signaling pathway reveals a bi-directional regulation effect on cell proliferation and osteogenic differentiation, and enhances senescence and neural differentiation, but inhibits adipogenic differentiation; non-canonical Wnt signaling pathway enhances senescence and osteogenic differentiation, and inhibits proliferation and adipogenic differentiation of mesenchymal stem cells, but it takes no part in neural differentiation of mesenchymal stem cells. So the regulations of Wnt signaling pathway on the biological characteristics of mesenchymal stem cells can be used as the new therapeutic targets of bone tissue engineering, nerve injury repair, and so on.

    中国组织工程研究杂志出版内容重点:干细胞;骨髓干细胞;造血干细胞;脂肪干细胞;肿瘤干细胞;胚胎干细胞;脐带脐血干细胞;干细胞诱导;干细胞分化;组织工程


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