Relationship between CD4+CD25+FOXP3+Treg cells and acute graft-versus-host disease

doi:10.3969/j.issn.2095-4344.2014.41.018

Abstract

Abstract:

BACKGROUND: The CD4⁺CD25⁺FOXP3⁺Treg cells have immunosuppression effect, and it is speculated that these cells may restrain the occurrence of acute graft-versus-host disease.

OBJECTIVE: To observe the variety of the CD4⁺CD25⁺FOXP3⁺Treg cells in the peripheral blood from donors before and after granulocyte colony stimulating factor mobilization, and study the relationship between CD4⁺CD25⁺FOXP3⁺Treg cells and acute graft-versus-host disease.

METHODS: Ninety patients with malignant blood diseases who undertook allogeneic hematopoietic stem cell transplantation and their donors were observed. Granulocyte colony stimulating factor 5 μg/kg was injected subcutaneously into the donor per 12 hours for 5 days, and the stem cells were collected before and after mobilization. The ratio of CD4⁺CD25⁺ FOXP3⁺Treg cells in the peripheral blood was detected before and after mobilization with flow cytometry, and the ratio of these cells in the stem cell suspension was measured by the same method. The patients were divided into two groups according to the CD4⁺CD25⁺ FOXP3⁺Treg cells ratio: high dosage group (≥5%) and low dosage group (< 5%). The incidence of acute graft-versus-host disease was observed in the two groups after transplantation.

RESULTS AND CONCLUSION: The ratio of the CD4⁺CD25⁺FOXP3⁺Treg cells in the donor before and after mobilization were 11.3% and 1.5%,respectively, and there was a significant difference (P < 0.05). The ratio of the CD4⁺CD25⁺FOXP3⁺Treg cells was 3.4% in the patients with acute graft-versus-host disease, and 15.7% in the patients with no acute graft-versus-host disease, showing a significant difference (P < 0.05). After hematopoietic reconstitution, the incidence of acute graft-versus-host disease was 18.4% in the high dosage group and 48.1% in the low dosage group, and there was a significant difference between the two groups (P < 0.05). Therefore, the granulocyte colony stimulating factor can lower the ratio of CD4⁺CD25⁺FOXP3⁺Treg cells in the human peripheral blood. The increase in CD4⁺CD25⁺FOXP3⁺Treg cells can restrain the occurrence of acute graft-versus-host disease.

中国组织工程研究杂志出版内容重点：干细胞；骨髓干细胞；造血干细胞；脂肪干细胞；肿瘤干细胞；胚胎干细胞；脐带脐血干细胞；干细胞诱导；干细胞分化；组织工程

全文链接：

Key words: hematopoietic stem cell transplantation, T-Lymphocytes, regulatory, graft vs host disease

CLC Number:

R394.2

Xie Xin-sheng, Wan Ding-ming, Sun Hui, Sun Ling, Zhang Qiu-tang. Relationship between CD4⁺CD25⁺FOXP3⁺Treg cells and acute graft-versus-host disease[J]. Chinese Journal of Tissue Engineering Research, 2014, 18(41): 6661-6665.

Figures/Tables 1

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