Drynaria freeze-dried powder at different dosages influences proliferation and differentiation of rabbit bone marrow mesenchymal stem cells

doi:10.3969/j.issn.2095-4344.2014.41.016

Abstract

Abstract:

BACKGROUND: Modern research shows that Drynaria can delay cell degeneration and reduce the incidence of osteoarthritis.

OBJECTIVE: To observe the proliferation and differentiation of rabbit bone marrow mesenchymal stem cells induced by different dosages of Drynaria freeze-dried powder, and to explore the optimum induction concentration.

METHODS: Rabbit bone marrow mesenchymal stem cells were isolated and cultured in vitro by density gradient centrifugation and adherence screening methods, and then divided into blank group, positive control group (transforming growth factor β1), high-, middle-, low-dosage Drynaria groups (0.4 mg, 0.1 mg, 5 μg). Passage 3 cells were selected and cultured in different media. After 1 week, cell viability was detected by MTT method, and expression of type II collagen by immunohistochemical method.

RESULTS AND CONCLUSION: Both transforming growth factor β1 and Drynaria could improve the proliferation of bone marrow mesenchymal stem cells, and the increase in cell proliferation was ranked as follows: positive control group > low-dosage group > middle-dosage group > high-dosage group > blank group. Bone marrow mesenchymal stem cells were differentiated into chondrocytes under induction of transforming growth factor β1 and Drynaria, and induced cells significantly expressed type II collagen. The expression of type II collagen was ranked as follows: positive control group > low-dosage group > middle-dosage group > high-dosage group > blank group. These findings suggest that Drynaria can promote the proliferation and differentiation of rabbit bone marrow mesenchymal stem cells, and the optimal dosage is 5 μg.

中国组织工程研究杂志出版内容重点：干细胞；骨髓干细胞；造血干细胞；脂肪干细胞；肿瘤干细胞；胚胎干细胞；脐带脐血干细胞；干细胞诱导；干细胞分化；组织工程

全文链接：

Key words: bone marrow, mesenchymal stem cells, polypodiaceae, cell proliferation

CLC Number:

R394.2

Zhang Hu-lin, Zhang Zi-qiang, Xu Hong-bin, Zhang Xiao-gang. Drynaria freeze-dried powder at different dosages influences proliferation and differentiation of rabbit bone marrow mesenchymal stem cells[J]. Chinese Journal of Tissue Engineering Research, 2014, 18(41): 6649-6654.

Figures/Tables 2

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