Chinese Journal of Tissue Engineering Research ›› 2015, Vol. 19 ›› Issue (14): 2211-2215.doi: 10.3969/j.issn.2095-4344.2015.14.014
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石家庄市第一医院,1胸外科,2内分泌科,3心内科,河北省石家庄市 050000
Revised:
2015-03-09
Online:
2015-04-02
Published:
2015-04-02
Contact:
Lu Cai-ping, Master, Attending physician, Department of Endocrinology, First Hospital of Shijiazhuang, Shijiazhuang 050000, Hebei Province, China
About author:
Zhao Xiao-jian, Master, Attending physician, Department of Thoracic Surgery, First Hospital of Shijiazhuang, Shijiazhuang 050000, Hebei Province, China
Zhao Xiao-jian, Lu Cai-ping, Chu Wei-wei, Zhang Ya-xiao, Zhang Bing, Zhen Qiang, Tan Guo-liang, Wang Ren-feng, Liu Jia-bao, Wu Lin. Bone marrow mesenchymal stem cell transplantation for treatment of emphysema: intravenous versus intratracheal approach[J]. Chinese Journal of Tissue Engineering Research, 2015, 19(14): 2211-2215.
2.1 实验动物数量分析 实验中纳入Wistar大鼠总数25只,其中1只用于提取骨髓间充质干细胞,其余24只大鼠造模成功率100%,均进入结果分析。 2.2 骨髓间充质干细胞形态及鉴定 细胞悬液中骨髓间充质干细胞呈圆形,悬浮状态。24 h后出现贴壁细胞,72 h后贴壁细胞多呈三角形、多角形或梭形,胞体饱满,胞浆半透明,细胞核呈圆形,核仁明显,常向外伸出2-4个长短不同突起。培养7 d时胞体增大,细胞形状逐渐以梭形为主,相互之间连接更加紧密,见图1。传代后细胞生长速度加快,密度增大,形态较均一,为长梭形,呈漩涡状或平行生长,光性较强。培养10-14 d时细胞达80%融合,细胞呈平行排列生长或漩涡状生长。流式细胞仪检测细胞表面标志表达阳性率,CD44为84.69%,CD90为90.54%,CD71为70.32%,CD45为2.87%[12]。"
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