DNA oxidative damage to bone marrow hematopoietic cells and apoptosis of osteocytes in early avascular necrosis of the femoral head induced by glucocorticoids

doi:10.3969/j.issn.2095-4344.2015.11.003

Abstract

Abstract:

BACKGROUND: Hormones-induced avascular necrosis of the femoral head is a complex biological process, and its pathogenesis remains unclear. To prove its etiologic mechanism is still the focus of research in this field.

OBJECTIVE: To discuss the pathological changes of hematopoietic cells and osteocytes in early avascular necrosis of the femoral head induced by glucocorticoids .

METHODS: Forty Japanese white rabbits, 4 months old, were randomly divided into glucocorticoids+ lipopolysaccharide group (combination group), glucocorticoids group, lipopolysaccharide group and blank control group, with 10 rabbits in each group. Five animals from each group were sacrificed at 2 and 4 weeks after the last dosing, respectively. Vacant bone lacunae were counted under optical microscope, morphology of osteocytes was observed under electron microscope, osteocyte apoptosis was assay by TUNEL, and DNA oxidative damage to hematopoietic cells was detected by immunohistochemistry.

RESULTS AND CONCLUSION: Compared with the other three groups, the rates of vacant lacunae and DNA oxidative damage to hematopoietic cells in the combination group were significantly higher at 2 and 4 weeks after treatment as well as the apoptosis rate of osteocytes at 4 weeks after treatment (P < 0.01); while there were no statistical differences between the other three groups. In the combination group, the vacant rate of bone lacunae and apoptosis rate of osteocytes were positively correlated (r=0.793). These findings indicate that DNA oxidative damage to hematopoietic cells and apoptosis of osteocytes participate in the pathological changes of early avascular necrosis of the femoral head induced by glucocorticoids. The former is prior to the latter, and the death manner of osteocytes is apoptosis.

中国组织工程研究杂志出版内容重点：组织构建；骨细胞；软骨细胞；细胞培养；成纤维细胞；血管内皮细胞；骨质疏松；组织工程

全文链接：

Key words: Femur Head Necrosis, Glucocorticoids, Apoptosis

CLC Number:

R318

Zhao Zhen-qun, Liu Wan-lin, Gong Yu-lin, Bai Rui, Wang Wen-xuan. DNA oxidative damage to bone marrow hematopoietic cells and apoptosis of osteocytes in early avascular necrosis of the femoral head induced by glucocorticoids[J]. Chinese Journal of Tissue Engineering Research, 2015, 19(11): 1652-1657.

Figures/Tables 3

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