Hyperbaric oxygen combined with Schwann cells transplantation for spinal cord injury in rats: electrophysiological and functional changes of the hind limbs

doi:10.3969/j.issn.2095-4344.2015.14.013

Abstract

Abstract:

BACKGROUND: Hyperbaric oxygen therapy can improve the microenvironment of the injured spinal cord, and hyperbaric oxygen combined with Schwann cell transplantation is expected to improve the therapeutic efficacy on spinal cord injury.

OBJECTIVE: To investigate the effect of Schwann cell transplantation plus hyperbaric oxygen on the neural functional recovery of rats with spinal cord injury.

METHODS: A total of 80 female SD rats with spinal cord injury were randomized into 4 groups, with 20 in each group: blank control group, injection of L-DMEM via the tail vein at 6 hours after modeling; cell transplantation group, injection of 3×10⁶ Schwann cell suspension via the tail vein at 6 hours after modeling; hyperbaric oxygen group, hyperbaric oxygen therapy at 1 hour after modeling; combination group, combined therapy of Schwann cell transplantation and hyperbaric oxygen. Inclined plane test, modified Tarlov score, Basso-Beattie-Bresnahan score for motor function evaluation of rat hind limbs were performed and measured at 1, 3 days, 1, 2, 3, 4 weeks after treatment. SRY gene expression in the spinal cord was measured at 4 weeks after transplantation using PCR method. Horseradish peroxidase tracer and electroneurophysiology detection was done at 8 weeks after transplantation.

RESULTS AND CONCLUSION: The motor function of the lower limbs was better in the combination group than the cell transplantation and hyperbaric oxygen groups, as well as better in the cell transplantation group and hyperbaric oxygen groups than the blank control group. SRY expression was detected in the cell transplantation group and combination group, but not in the blank control group and hyperbaric oxygen group. The number of nerve fibers positive for horseradish peroxidase was higher in the combination group than the cell transplantation and hyperbaric oxygen groups followed by the blank control group, and there were significant differences between different groups (P < 0.01). In addition, the latencies and amplitudes of somatosensory evoked potential and motor evoked potential in the combination group were also better than those in the other groups (P < 0.05 or P < 0.01). These findings indicate that the combined therapy of Schwann cell transplantation and hyperbaric oxygen can promote the synaptic regeneration, improve limb motor function and electrophysiological function in rats with spinal cord injury, which is superior to hyperbaric oxygen or Schwann cell transplantation alone.

中国组织工程研究杂志出版内容重点：干细胞；骨髓干细胞；造血干细胞；脂肪干细胞；肿瘤干细胞；胚胎干细胞；脐带脐血干细胞；干细胞诱导；干细胞分化；组织工程

全文链接：

Key words: Schwann Cells, Hyperbaric Oxygenation, Spinal Cord Injuries, Genes, sry

CLC Number:

R394.2

Wang Guo-xi, Wang Guo-qian, Zhang Shu-quan. Hyperbaric oxygen combined with Schwann cells transplantation for spinal cord injury in rats: electrophysiological and functional changes of the hind limbs [J]. Chinese Journal of Tissue Engineering Research, 2015, 19(14): 2205-2210.

Figures/Tables 4

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