Expression of CD44+/C-myc+ cancer stem cells and its relationship with the prognosis of patients in colorectal tumors

doi:10.3969/j.issn.2095-4344.2015.14.006

Abstract

Abstract:

BACKGROUND: Tumor recurrence results from the incomplete removal of cancer stem cells with self-renewal characteristics, and then how to label and eliminate cancer stem cells becomes the key to cancer treatment.

OBJECTIVE: To observe the morphology，distribution and number of CD44⁺/C-myc⁺ cells in colorectal cancer, and to explore the relationship between the expression and postoperative metastasis.

METHODS: Pathological tissues from 150 patients with colorectal cancer were taken to prepare tissue microarray, in order to observe and count CD44⁺/C-myc⁺ cells by using immunohistochemical double staining. Patients were followed up through mobile phones , letters , and so on, and the relationship between the expression of CD44⁺/C-myc⁺ cells and postoperative metastasis were statistically recorded.

RESULTS AND CONCLUSION: There was no CD44⁺/C-myc⁺ cells in normal tissue and little in adenoma. A small number of CD44⁺/C-myc⁺ cells distributed as dots or focal lesions in adenocarcinoma. The number of CD44⁺/C-myc⁺ cells was related to the degree of adenocarcinoma differentiation, depth of invasion, and lymph node metastasis (P < 0.05). The univariate analysis showed that the overall survival rate and progression-free survival rate were associated with the number of CD44⁺/C-myc⁺ cells, lymph node metastasis and Ducks staging in adenocarcinoma (P < 0.05). The multivariate analysis showed that the overall survival rate was related to CD44⁺/C-myc⁺ cell amount and the progression-free survival rate was related to CD44⁺/C-myc⁺ cell amount and the Ducks staging. Therefore, CD44⁺/C-myc⁺ cells are probably cancer stem cells, and Ducks staging and CD44⁺/C-myc⁺ cell amount are important prognostic factors for colorectal cancer.

中国组织工程研究杂志出版内容重点：干细胞；骨髓干细胞；造血干细胞；脂肪干细胞；肿瘤干细胞；胚胎干细胞；脐带脐血干细胞；干细胞诱导；干细胞分化；组织工程

全文链接：

Key words: Colorectal Neoplasms, Neoplastic Stem Cells, Genes, myc;, Cell Adhesion Molecules

CLC Number:

R394.2

Ma Fa-ku, Wang Huan, Liu Bin, Yang Yan-li, Su Qin-jun, Qian Zhen, Dong Liang. Expression of CD44⁺/C-myc⁺ cancer stem cells and its relationship with the prognosis of patients in colorectal tumors[J]. Chinese Journal of Tissue Engineering Research, 2015, 19(14): 2161-2166.

Figures/Tables 5

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