siRNA lentiviral vectors carrying telomerase reverse transcriptase gene hasten astrocytes apoptosis

doi:10.3969/j.issn.2095-4344.2015.11.013

Abstract

Abstract:

BACKGROUND: Telomerase reverse transcriptase plays an important role in telomerase activation, and lentiviral vectors carrying human telomerase reverse transcriptase that can inhibit astrocyte expression are rarely reported to have effects on spinal cord injury.

OBJECTIVE: To transfect rat astrocytes with telomerase reverse transcriptase gene lentiviral vectors and to observe the effect of telomerase reverse transcriptase gene lentiviral vectors on apoptosis of astrocytes.

METHODS: Astrocytes from rats were subject to primary culture and subculture, and then transfected with siRNA lentiviral vector carrying telomerase reverse transcriptase gene (siRNA transfection group), simple lentiviral vector (lentiviral vector group) and nothing (blank group), respectively. Then, the transfection efficiency and apoptosis in different time periods after transfection were determined.

RESULTS AND CONCLUSION: The transfection efficiency was up to 85%-90% after siRNA lentiviral vector and simple lentiviral vector transfection. The apoptosis rate of astrocytes in the siRNA transfection group was 50%-60% at 24-48 hours after transfection, but there was no significant change in the other two groups. These findings suggest that siRNA lentiviral vectors carrying telomerase reverse transcriptase gene can accelerate astrocytes apoptosis.

中国组织工程研究杂志出版内容重点：组织构建；骨细胞；软骨细胞；细胞培养；成纤维细胞；血管内皮细胞；骨质疏松；组织工程

全文链接：

Key words: Tissue Engineering, Genes, Cells, Spinal Cord Injuries

CLC Number:

R318

Liang Liang, Xu Tao, Song Yang, Sheng Wei-bin. siRNA lentiviral vectors carrying telomerase reverse transcriptase gene hasten astrocytes apoptosis[J]. Chinese Journal of Tissue Engineering Research, 2015, 19(11): 1707-1711.

Figures/Tables 2

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